Recombinant Bovine IL-10 Protein

• Reactivity: Bv
• Applications: ELISA
• Concentration: LYOPH

Recombinant Bovine IL-10 Protein Summary

• Description: Recombinant bovine interleukin-10.
  
  

  Source: Pichia pastoris

  Amino Acid Sequence: 160 amino acid sequence of bovine IL-10, UniProt P43480

• Preparation Method: Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography.
• Source: Pichia pastoris
• Protein/Peptide Type: Recombinant Protein
• Gene: IL10
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • ELISA
• Theoretical MW: 18.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. After reconstitution store at -20C.
• Buffer: PBS
• Preservative: 10% Trehalose
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute with 100 ul sterile PBS containing 0.1% carrier protein. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix vial after reconstitution.

Alternate Names for Recombinant Bovine IL-10 Protein

• CSIF
• CSIFMGC126450
• Cytokine synthesis inhibitory factor
• GVHDS
• IL10
• IL-10
• IL10A
• IL-10MGC126451
• interleukin 10
• interleukin-10
• TGIF

Background

Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. ILs have a variety of functions, but most function to direct other immune cells to divide and differentiate. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Human IL10 is a non glycosylated polypeptide consisting of 178 amino acids. There is 73% homology between the human and mouse IL10 proteins, however, the human IL10 acts on both human and mouse target cells, while the mouse IL10 has species specific activity. The cellular sources of IL10 are CD4+ T cells and T cell clones, thymocytes, B cells and B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL10 will stimulate the growth of stem cells, mast cells and thymocytes. IL10 enhances cytotoxic T cell development, and costimulates B cell differentiation and immunoglobulin secretion. IL10 inhibits cytokine production by macrophages and suppresses macrophage class II MHC expression. The human IL10 gene is on human chromosome 1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: IL10
• Uniprot:
  • P43480(Bovine)