Monkey IL-10 ELISA Kit (Colorimetric)

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ELISA: Monkey IL-10 ELISA Kit (Colorimetric) [NBP1-92708]

Product Details

Summary
Reactivity PmSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum (baboon, cynomolgus)
Standard Curve Range
3.9 - 250 U/mL
Sensitivity
1.3 U/mL

Order Details

Monkey IL-10 ELISA Kit (Colorimetric) Summary

Description
The monkey IL-10 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of monkey IL-10.
Specificity
The interference of circulating factors of the immune systemwas evaluated by spiking these proteins at physiologically relevant concentrations into a monkey IL-10 positive serum. There was no crossreactivity detected.
Standard Curve Range
3.9 - 250 U/mL
Sensitivity
1.3 U/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 10%
Intra-Assay
CV% < 5.0%
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
IL10

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Plate Covers
  2. Aluminium pouch(es) with a Microwell Plate coated with Monoclonal Antibody (murine) to monkey IL-10, Biotin-Conjugate (anti-monkey IL-10 monoclonal antibody), Sample Diluent and Streptavidin-HRP, lyophilized
  3. Aluminium pouch(es) with a monkey IL-10 Standard curve (coloured)
  4. Sample Diluent (Use when an external predilution of the samples is needed)
  5. Stop Solution (1M Phosphoric acid)
  6. Substrate Solution (tetramethyl-benzidine)
  7. Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)

Alternate Names for Monkey IL-10 ELISA Kit (Colorimetric)

  • CSIF
  • CSIFMGC126450
  • Cytokine synthesis inhibitory factor
  • GVHDS
  • IL10
  • IL-10
  • IL10A
  • IL-10MGC126451
  • interleukin 10
  • interleukin-10
  • TGIF

Background

Interleukin-10 is a pleiotropic cytokine playing an important role as a regulator of lymphoid and myeloid cell function. Due to the ability of IL-10 to block cytokine synthesis and several accessory cell functions of macrophages this cytokine is a potent suppressor of the effector functions of macrophages, T-cells and NK cells. In addition, IL-10 participates in regulating proliferation and differentiation of B-cells, mast cells and thymocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol IL10