IL-10 Antibody - EBV

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Product Details

Summary
Reactivity VSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

IL-10 Antibody - EBV Summary

Immunogen
E. coli-derived recombinant viral EBV IL‑10
Gln26-Arg170
Accession # P03180
Specificity
Detects viral EBV IL‑10 in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant canine IL‑10 and recombinant human IL‑10 is observed and less than 10% cross-reactivity with recombinant mouse IL‑10 and recombinant rat IL‑10 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CeHV15gBCRF1
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
Publications
Read Publication using
AF915 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-10 Antibody - EBV

  • CSIF
  • CSIFMGC126450
  • Cytokine synthesis inhibitory factor
  • GVHDS
  • IL10
  • IL-10
  • IL10A
  • IL-10MGC126451
  • interleukin 10
  • interleukin-10
  • TGIF

Background

Interleukin-10 (IL-10) is a pleiotropic cytokine that plays a crucial role in regulating immune and inflammstory responses (1). It has closely related homologs in the genome of several viruses including the human cytomegalovirus (HCMV), a prevalent pathogen that can remain in humans as a latent infection (2). Viruses use multiple strategies to avoid detection and clearance by the host immune system. One of these strategies is to express virus-encoded host cytokine homologs, such as IL-10, to modulate the host immune system to the virus’ advantage. HCMV IL-10 is encoded from noncontiguous stretches in the viral genome and is transcribed early during infection. It shares 27% amino acid sequence identity with the endogenous human IL-10. Despite its low homology with the human protein, it can bind to the human IL-10 receptor complex and induces signal transduction. HCMV IL-10 has been shown to suppress cytokine production and inhibit proliferation of mitogen‑stimulated human peripheral blood mononuclear cells. HCMV IL-10 can also down regulate the antigen-presenting and accessory cell functions of human monocytes. These immunosuppressive properties of HCMV IL-10 likely contribute to immune evasion during virus infection (3).

  1. Moore, K.W. et al. (2001) Annu. Rev. Immunol. 19:683.
  2. Kotenko, S.V. et al. (2000) Proc. Natl. Acad. Sci. USA 97:1695.
  3. Spencer, J.V. et al. (2002) J. Virol. 76:1285.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-10 Antibody (AF915)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ELISA Development.


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Success of combined IL-10 and IL-12 therapy in colon cancer depends on IFN-gamma and gut barrier integrity
By Jamshed Arslan, Pharm. D., PhD. Colon cancer is responsible for over 600,000 deaths per year worldwide. Colon cancer can be classified into two categories: mismatch repair (MMR)-deficient and MMR-proficient cancers...  Read full blog post.

How to identify B cell subsets using flow cytometry
By Victoria OsinskiUsing flow cytometry to identify B cell subsetsIdentifying cellular subsets by flow cytometry requires careful and thorough planning in order to ensure the correct subset of cells are identified a...  Read full blog post.

PPAR gamma - An important target in human metabolism
Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells by interacting with members of the nuclear hormone receptor superfamily known as peroxisome proliferator activated receptors (PPARs). There are f...  Read full blog post.

CD11b: Marker for a New Type of B Cell that Participates in Cell-Mediated Immunity
Think B lymphocytes just produce antibodies? Think again! Although, of course, B cells are vital for the humoral immune response, many studies in recent years have begun to uncover antibody-independent actions of B cells: regulating T cells and thus a...  Read full blog post.

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Bioinformatics

Gene Symbol CeHV15gBCRF1
Uniprot