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IL-10 Antibody [Unconjugated] - HCMV

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Recombinant Viral HCMV IL‑10 (Catalog # 117-VL) inhibits LPS-indecued IL-1 beta secretion in human peripheral blood lymphocytes (PBL) in a dose-dependent manner (orange line). IL-1 beta secretion inhibited by ...read more

Product Details

Summary
Reactivity VSpecies Glossary
Applications WB, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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IL-10 Antibody [Unconjugated] - HCMV Summary

Immunogen
E. coli-derived recombinant viral HCMV IL‑10
Ala26-Lys176
Accession # AAF63437
Specificity
Detects viral HCMV IL-10 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse IL‑10, recombinant canine IL‑10, recombinant feline IL‑10, recombinant cotton rat IL‑10, recombinant rat IL‑10, recombinant porcine IL‑10, recombinant human IL‑10, and recombinant Epstein-Barr virus encoded viral IL‑10 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CeHV15gBCRF1
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Neutralization 0.15-0.9 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF117 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-10 Antibody [Unconjugated] - HCMV

  • CSIF
  • CSIFMGC126450
  • Cytokine synthesis inhibitory factor
  • GVHDS
  • IL10
  • IL-10
  • IL10A
  • IL-10MGC126451
  • interleukin 10
  • interleukin-10
  • TGIF

Background

Interleukin-10 (IL-10) is a pleiotropic cytokine that plays a crucial role in regulating immune and inflammstory responses (1). It has closely related homologs in the genome of several viruses including the human cytomegalovirus (HCMV), a prevalent pathogen that can remain in humans as a latent infection (2). Viruses use multiple strategies to avoid detection and clearance by the host immune system. One of these strategies is to express virus-encoded host cytokine homologs, such as IL-10, to modulate the host immune system to the virus’ advantage. HCMV IL-10 is encoded from noncontiguous stretches in the viral genome and is transcribed early during infection. It shares 27% amino acid sequence identity with the endogenous human IL-10. Despite its low homology with the human protein, it can bind to the human IL-10 receptor complex and induces signal transduction. HCMV IL-10 has been shown to suppress cytokine production and inhibit proliferation of mitogen-stimulated human peripheral blood mononuclear cells. HCMV IL-10 can also down regulate the antigen-presenting and accessory cell functions of human monocytes. These immunosuppressive properties of HCMV IL-10 likely contribute to immune evasion during virus infection (3).

  1. Moore, K.W. et al. (2001) Annu. Rev. Immunol. 19:683.
  2. Kotenko, S.V. et al. (2000) Proc. Natl. Acad. Sci. USA 97:1695.
  3. Spencer, J.V. et al. (2002) J. Virol. 76:1285.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-10 Antibody (AF117)(9)

We have publications tested in 2 confirmed species: Human, Virus.

We have publications tested in 3 applications: ELISA Capture, ELISA Development, Western Blot.


Filter By Application
ELISA Capture
(1)
ELISA Development
(3)
Western Blot
(3)
All Applications
Filter By Species
Human
(3)
Virus
(3)
All Species
Showing Publications 1 - 9 of 9.
Publications using AF117 Applications Species
Joseph L. Wiemels, Rong Wang, Mi Zhou, Helen Hansen, Rachel Gallant, Junghyun Jung et al. Cytomegalovirus proteins, maternal pregnancy cytokines, and their impact on neonatal immune cytokine profiles and acute lymphoblastic leukemogenesis in children Haematologica 2022-05-31 [PMID: 35638549]
S Waters, S Lee, A Irish, P Price Challenging the Conventional Interpretation of HCMV Seronegativity Microorganisms, 2021-11-18;9(11):. 2021-11-18 [PMID: 34835508] (ELISA Capture, Human) ELISA Capture Human
J Stuchl?, V Kanderov , M Vlkov , I He?manov , L Sl mov , O Pel k, E Taraldsrud, D J¡lek, P Kr l¡c Kov, B Fevang, M Trkov , O Hru? k, E Fro?kov , A ?ediv , J Litzman, T Kalina Common Variable Immunodeficiency patients with a phenotypic profile of immunosenescence present with thrombocytopenia Sci Rep, 2017-01-05;7(0):39710. 2017-01-05 [PMID: 28054583] (ELISA Development, Human) ELISA Development Human
Valle Oseguera , Cendy A, Spencer , Juliet V cmvIL-10 stimulates the invasive potential of MDA-MB-231 breast cancer cells. PLoS ONE, 2014-02-10;9(2):e88708. 2014-02-10 [PMID: 24520416] (Western Blot, Human) Western Blot Human
Forster S, Brandt M, Mottok D, Zschuttig A, Zimmermann K, Blattner F, Gunzer F, Pohlmann C Secretory expression of biologically active human Herpes virus interleukin-10 analogues in Escherichia coli via a modified Sec-dependent transporter construct. BMC Biotechnol, 2013-10-05;13(0):82. 2013-10-05 [PMID: 24093616] (ELISA Development, Western Blot, Virus) ELISA Development, Western Blot Virus
Orin Bloch, Courtney A. Crane, Rajwant Kaur, Michael Safaee, Martin J. Rutkowski, Andrew T. Parsa Gliomas Promote Immunosuppression through Induction of B7-H1 Expression in Tumor-Associated Macrophages Clinical Cancer Research 2013-06-15 [PMID: 23613317]
Noelle D. Brodeur, Juliet V. Spencer Antibodies to human IL-10 neutralize ebvIL-10-mediated cytokine suppression but have no effect on cmvIL-10 activity Virus Research 2010-11-01 [PMID: 20728483]
Jenkins C, Garcia W, Godwin MJ, Spencer JV, Stern JL, Abendroth A, Slobedman B Immunomodulatory properties of a viral homolog of human interleukin-10 expressed by human cytomegalovirus during the latent phase of infection. J. Virol., 2008-01-23;82(7):3736-50. 2008-01-23 [PMID: 18216121] (Western Blot, Virus) Western Blot Virus
Chang WL, Baumgarth N, Yu D, Barry PA Human cytomegalovirus-encoded interleukin-10 homolog inhibits maturation of dendritic cells and alters their functionality. J. Virol., 2004-08-01;78(16):8720-31. 2004-08-01 [PMID: 15280480] (ELISA Development, Virus) ELISA Development Virus

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Bioinformatics

Gene Symbol CeHV15gBCRF1
Uniprot