IL-10 Antibody

Recombinant Mouse IL‑10 (Catalog # 417-ML) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑10 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑10 Polyclonal Antibody (Catalog # AB-417-NA). The ND50 is typically 2-4 µg/mL.

• Reactivity: Mu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-10 Antibody Summary

• Immunogen: E. coli-derived recombinant mouse IL‑10
  Ser19-Ser178
  Accession # NP_034678
• Specificity: Detects mouse IL-10 in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human IL‑10 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL10
• Purity Statement: Protein A or G purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 2-4 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 1 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-10 Antibody

• CSIF
• CSIFMGC126450
• Cytokine synthesis inhibitory factor
• GVHDS
• IL10
• IL-10
• IL10A
• IL-10MGC126451
• interleukin 10
• interleukin-10
• TGIF

Background

Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of alpha -helical cytokines that also includes IL-19, IL‑20, IL-22, and IL-24 (1, 2). IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts (2‑5). Mature mouse IL-10 shares 85% amino acid sequence identity with rat and 70%‑77% with bovine, canine, equine, feline, human, ovine, and porcine IL-10. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (6, 7). IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer (8‑10). The IL-10 dimer binds to two IL-10 R alpha /IL-10 R1 chains, resulting in recruitment of two IL-10 R beta /IL-10 R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3 (11). IL-10 R beta does not bind IL-10 by itself but is required for signal transduction (1). IL-10 R beta also associates with IL‑20 R alpha , IL-22 R alpha , or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL‑29 (12‑14). IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation (15‑17). It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses (2).

1. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
2. O’Garra, A. and P. Vieira (2007) Nat. Rev. Immunol. 7:425.
3. Mathurin, P. et al. (2002) Am. J. Physiol. Gastrointest. Liver Physiol. 282:G981.
4. Grewe, M. et al. (1995) J. Invest. Dermatol. 104:3.
5. Szony, B.J. et al. (1999) Mol. Hum. Reprod. 5:1059.
6. Vieira, P. et al. (1991) Proc. Natl. Acad. Sci. 88:1172.
7. Hsu, D.-H. et al. (1990) Science 250:830.
8. Moore, K. et al. (1990) Science 248:1230.
9. Windsor, W.T. et al. (1993) Biochemistry 32:8807.
10. Syto, R. et al. (1998) Biochemistry 37:16943.
11. Kotenko, S.V. et al. (1997) EMBO J. 16:5894.
12. Kotenko, S.V. et al. (2000) J. Biol. Chem. 276:2725.
13. Hor, S. et al. (2004) J. Biol. Chem. 279:33343.
14. Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
15. Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372.
16. Wu, K. et al. (2007) Cell. Mol. Immunol. 4:269.
17. Blackburn, S.D. and E.J. Wherry (2007) Trends Microbiol. 15:143.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-10 Antibody (AB-417-NA)(5)

Publications using AB-417-NA

• Zhu L, Huang Z, Stalesen R, Hansson G, Li N Platelets provoke distinct dynamics of immune responses by differentially regulating CD4+ T-cell proliferation. J Thromb Haemost, 2014-06-27;12(7):1156-65. 2014-06-27 [PMID: 24833264] (Bioassay, Human)
• Yuejuan Zheng, Yang Yang, Yuhu Li, Limin Xu, Yi Wang, Ziyi Guo et al. Ephedrine hydrochloride inhibits PGN-induced inflammatory responses by promoting IL-10 production and decreasing proinflammatory cytokine secretion via the PI3K/Akt/GSK3 beta pathway Cellular & Molecular Immunology 7/1/2013 [PMID: 23604046]
• Yuejuan Zheng, Yang Yang, Yuhu Li, Limin Xu, Yi Wang, Ziyi Guo et al. Ephedrine hydrochloride inhibits PGN-induced inflammatory responses by promoting IL-10 production and decreasing proinflammatory cytokine secretion via the PI3K/Akt/GSK3 beta pathway Cellular & Molecular Immunology 2013-07-01 [PMID: 23604046] (Neutralization, Mouse)
• Matsumura N, Kamei M, Tsujikawa M Low-Dose Lipopolysaccharide Pretreatment Suppresses Choroidal Neovascularization via IL-10 Induction. PLoS ONE, 2012-07-03;7(7):e39890. 2012-07-03 [PMID: 22802947] (In Vivo, Mouse)
• Wang H, Cheng F Histone Deacetylase Inhibitor LAQ824 Augments Inflammatory Responses in Macrophages through Transcriptional Regulation of IL-10. J. Immunol., 2011-03-02;186(0):3986. 2011-03-02 [PMID: 21368229] (Neutralization, Mouse)

Bioinformatics

• Gene Symbol: IL10
• Uniprot:
  • NP_034678()