IL-10 Antibody (2050B) [Unconjugated]

Human peripheral blood mononuclear cells (PBMCs) either (A) stimulated to induce Th2 cells or (B) unstimulated were stained with Rabbit Anti-Human IL‑10 Monoclonal Antibody (Catalog # MAB9210) followed by Fluorescein-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0112) and Mouse Anti-Human CD4 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB3791N). Quadrant markers were set based on control antibody staining (Catalog # AB-105-C). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with methanol. View our protocol for Staining Intracellular Molecules.

Recombinant Human IL-10 (Catalog # 217-IL) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL-10 (5 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB9210). The ND50 is typically 75-450 ng/mL.

• Reactivity: Hu
• Applications: B/N, Flow
• Clone: 2050B
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

IL-10 Antibody (2050B) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-10
  Met1-Asn178
  Accession # P22301
• Specificity: Detects human IL-10 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rabbit
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Neutralization 75-450 ng/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-10 Antibody (2050B) [Unconjugated]

• CSIF
• CSIFMGC126450
• Cytokine synthesis inhibitory factor
• GVHDS
• IL10
• IL-10
• IL10A
• IL-10MGC126451
• interleukin 10
• interleukin-10
• TGIF

Background

Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL‑10 family of alpha ‑helical cytokines that also includes IL-19, IL‑20, IL‑22, IL‑24, and IL‑26/AK155 (1, 2). IL‑10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts (2‑5). Mature human IL‑10 shares 72%‑86% amino acid sequence identity with bovine, canine, equine, feline, mouse, ovine, porcine, and rat IL‑10. Whereas human IL‑10 is active on mouse cells, mouse IL‑10 does not act on human cells (6, 7). IL‑10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer (6, 8, 9). The IL‑10 dimer binds to two IL‑10 R alpha /IL‑10 R1 chains, resulting in recruitment of two IL‑10 R beta /IL‑10 R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3 (10). IL‑10 R beta does not bind IL‑10 by itself but is required for signal transduction (1). IL‑10 R beta also associates with IL‑20 R alpha, IL‑22 R alpha, or IL‑28 R alpha to form the receptor complexes for IL‑22, IL‑26, IL‑28, and IL‑29 (11‑13). IL‑10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation (14‑16). It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses (2).

1. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
2. Sabat, R. et al. (2010) Cytokine Growth Factor Rev. 21:331.
3. Mathurin, P. et al. (2002) Am. J. Physiol. Gastrointest. Liver Physiol. 282:G981.
4. Grewe, M. et al. (1995) J. Invest. Dermatol. 104:3.
5. Szony, B.J. et al. (1999) Mol. Hum. Reprod. 5:1059.
6. Vieira, P. et al. (1991) Proc. Natl. Acad. Sci. 88:1172.
7. Hsu, D.-H. et al. (1990) Science 250:830.
8. Windsor, W.T. et al. (1993) Biochemistry 32:8807.
9. Syto, R. et al. (1998) Biochemistry 37:16943.
10. Kotenko, S.V. et al. (1997) EMBO J. 16:5894.
11. Kotenko, S.V. et al. (2000) J. Biol. Chem. 276:2725.
12. Hor, S. et al. (2004) J. Biol. Chem. 279:33343.
13. Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
14. Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372.
15. Wu, K. et al. (2007) Cell. Mol. Immunol. 4:269.
16. Blackburn, S.D. and E.J. Wherry (2007)Trends Microbiol. 15:143.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-10 Antibody (MAB9210)(1)

Publication using MAB9210

• M Ma, X Yin, X Zhao, C Guo, X Zhu, T Liu, M Yang, Z Zhang, Y Fu, J Liu, J Xu, H Ding, X Han, Z Chu, H Shang, Y Jiang CD56- CD16+ NK cells from HIV-infected individuals negatively regulate IFN-γ production by autologous CD8+ T cells J. Leukoc. Biol., 2019;0(0):. 2019 [PMID: 31483071] (Flow Cytometry, Human)

Bioinformatics

• Uniprot:
  • P22301()