IL-1 RI Antibody [Fluorescein]

HUVEC human umbilical vein endothelial cells were stained with Goat Anti-Human IL-1 RI Fluorescein-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB269F, filled histogram) or control antibody (Catalog # IC108F, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Fluorescein

IL-1 RI Antibody [Fluorescein] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-1 RI
• Specificity: Detects human IL-1 RI in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL1R1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1 RI Antibody [Fluorescein]

• CD121 antigen-like family member A
• CD121a antigen
• CD121a
• IL-1 RI
• IL1R
• IL1R1
• IL1RI
• IL-1RI
• IL1RT1
• IL-1RT1
• IL-1RT-1
• Interleukin 1 Receptor 1
• interleukin 1 receptor, type I
• interleukin receptor 1
• interleukin-1 receptor type 1
• p80

Background

Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor Type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts, and endothelial cells. IL-1 receptor Type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence identity in their extracellular domains. The two receptor types do not heterodimerize into a receptor complex.

An IL-1 receptor accessory protein that can heterodimerize with the Type I receptor in the presence of IL-1 alpha or IL-1 beta but not IL-1ra, was identified (1). This Type I receptor complex appears to mediate all the known IL-1 biological responses. The receptor Type II has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the Type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling Type I receptor complex. Recombinant IL-1 soluble receptor Type I is a potent antagonist of IL-1 action.

1. Greenfeder, S. et al. (1995) J. Biol. Chem. 270:13757.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-1 RI Antibody (FAB269F)(9)

Publications using FAB269F

• Y Xu, M Twarog, N Li, A Banks, J Schustak, Y Bao, Q Huang, QG Medley Zinc transport from the endoplasmic reticulum to the cytoplasm via Zip7 is necessary for barrier dysfunction mediated by inflammatory signaling in RPE cells PLoS ONE, 2022-07-28;17(7):e0271656. 2022-07-28 [PMID: 35901031] (Flow Cytometry, Human)
• A Hackel, A Aksamit, K Bruderek, S Lang, S Brandau TNF-alpha and IL-1beta sensitize human MSC for IFN-gamma signalling and enhance neutrophil recruitment Eur. J. Immunol., 2020-09-24;0(0):. 2020-09-24 [PMID: 32845509] (Flow Cytometry, Human)
• Tongtian Zhuang, Shuli Li, Xiuli Yi, Sen Guo, Yinghan Wang, Jianru Chen et al. Tranilast Directly Targets NLRP3 to Protect Melanocytes From Keratinocyte-Derived IL-1 beta Under Oxidative Stress Frontiers in Cell and Developmental Biology 7/10/2020 [PMID: 32754591]
• M Bergström, M Müller, M Karlsson, H Scholz, NT Vethe, O Korsgren Comparing the Effects of the mTOR Inhibitors Azithromycin and Rapamycin on In Vitro Expanded Regulatory T Cells Cell Transplant, 2019-09-12;0(0):9636897198724. 2019-09-12 [PMID: 31512504] (Flow Cytometry, Human)
• Mythilypriya Rajendran, Stephen Looney, Nagendra Singh, Mahmoud Elashiry, Mohamed M. Meghil, Ahmed R. El-Awady et al. Systemic Antibiotic Therapy Reduces Circulating Inflammatory Dendritic Cells and Treg-Th17 Plasticity in Periodontitis. The Journal of Immunology 5/1/2019 [PMID: 30944162]
• Zhang Z, Cheng L, Zhao J, Li G, Zhang L, Chen W, Nie W, Reszka-Blanco N, Wang F, Su L Plasmacytoid dendritic cells promote HIV-1-induced group 3 innate lymphoid cell depletion. J Clin Invest, 2015-08-24;125(9):3692-703. 2015-08-24 [PMID: 26301812] (Flow Cytometry, Human)
• Zhang X, Tao Y, Chopra M, Dujmovic-Basuroski I, Jin J, Tang Y, Drulovic J, Markovic-Plese S IL-11 Induces Th17 Cell Responses in Patients with Early Relapsing-Remitting Multiple Sclerosis. J Immunol, 2015-04-20;194(11):5139-49. 2015-04-20 [PMID: 25895532] (Flow Cytometry, Human)
• Xia Xi, David H. McMillan, Geniece M. Lehmann, Patricia J. Sime, Richard T. Libby, Krystel R. Huxlin et al. Ocular Fibroblast Diversity: Implications for Inflammation and Ocular Wound Healing Investigative Opthalmology & Visual Science 7/1/2011 [PMID: 21571679]
• Lee WW, Kang SW, Choi J Regulating human Th17 cells via differential expression of IL-1 receptor. Blood, 2009-11-12;115(3):530-40. 2009-11-12 [PMID: 19965648] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: IL1R1