IL-1 RI Antibodies

VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing central nervous system. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing central nervous system. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="IL-1 RI was detected in perfusion fixed frozen sections of mouse skin (tail) using Goat Anti-Mouse IL-1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF771) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing central nervous system. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

400-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF771). The ND₅₀ is typically 0.2-1.0 µg/mL." class="big_lightbox"> 400-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF771). The ND₅₀ is typically 0.2-1.0 µg/mL." class="big_thumb" />

AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." class="big_lightbox"> AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." alt="HUVEC human umbilical vein endothelial cells were labeled with Human IL-1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF269, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." class="big_thumb" />

201-LB) induces CXCL1/GROa secretion in the HT-29 human colon adenocarcinoma cell line in a dose-dependent manner (orange line),as measured by the Human CXCL1/GROa DuoSet (Catalog # DY275). CXCL1/GROa secretion elicited by Recombinant Human IL-1 beta /IL-1F2 is neutralized (green line) by increasing concentrations of Human IL-1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF269). The ND₅₀ is typically < 5 µg/mL in the presence of 400 pg/mL Recombinant Human IL-1 beta /IL-1F2." class="big_lightbox"> 201-LB) induces CXCL1/GROa secretion in the HT-29 human colon adenocarcinoma cell line in a dose-dependent manner (orange line),as measured by the Human CXCL1/GROa DuoSet (Catalog # DY275). CXCL1/GROa secretion elicited by Recombinant Human IL-1 beta /IL-1F2 is neutralized (green line) by increasing concentrations of Human IL-1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF269). The ND₅₀ is typically < 5 µg/mL in the presence of 400 pg/mL Recombinant Human IL-1 beta /IL-1F2." class="big_thumb" />

269-1R) was serially diluted and captured by Mouse Anti-Human IL‑1 RI Monoclonal Antibody (Catalog # MAB269) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF269) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 269-1R) was serially diluted and captured by Mouse Anti-Human IL‑1 RI Monoclonal Antibody (Catalog # MAB269) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF269) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

269-1R) was serially diluted and captured by Mouse Anti-Human IL‑1 RI Monoclonal Antibody (Catalog # MAB269) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF269) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 269-1R) was serially diluted and captured by Mouse Anti-Human IL‑1 RI Monoclonal Antibody (Catalog # MAB269) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF269) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Human IL‑1 RI (Catalog # 269-1R) was serially diluted and captured by Mouse Anti-Human IL‑1 RI Monoclonal Antibody (Catalog # MAB269) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF269) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox"> MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins. " alt="RAW264 cells were stained with Rat Anti-Mouse IL‑1 RI Monoclonal Antibody (Catalog # MAB7712, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

HAF018). Specific bands were detected for IL-1 RI at approximately 70-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF018). Specific bands were detected for IL-1 RI at approximately 70-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IL-1 RI Monoclonal Antibody (Catalog # MAB2691) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for IL-1 RI at approximately 70-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />