IL-1 RI Antibody [Unconjugated]

Images

 
HUVEC human umbilical vein endothelial cells were labeled with Human IL‑1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF269, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), ...read more
Recombinant Human IL‑1 beta /IL‑1F2 (Catalog # 201-LB) inhibits proliferation in the A375s2 human melanoma cell line in a dose-dependent manner (orange line). Growth inhibition elicited by Recombinant Human IL‑1 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, B/N, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

IL-1 RI Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-1 RI
Specificity
Detects human IL-1 RI in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IL1R1
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready
  • Neutralization 0.5-2.0 ug/mL
Publications
Read Publications using
AF269 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1 RI Antibody [Unconjugated]

  • CD121 antigen-like family member A
  • CD121a antigen
  • CD121a
  • IL-1 RI
  • IL1R
  • IL1R1
  • IL1RI
  • IL-1RI
  • IL1RT1
  • IL-1RT1
  • IL-1RT-1
  • Interleukin 1 Receptor 1
  • interleukin 1 receptor, type I
  • interleukin receptor 1
  • interleukin-1 receptor type 1
  • p80

Background

Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor Type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts, and endothelial cells. IL-1 receptor Type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence identity in their extracellular domains. The two receptor types do not heterodimerize into a receptor complex.

An IL-1 receptor accessory protein that can heterodimerize with the Type I receptor in the presence of IL-1 alpha or IL-1 beta but not IL-1ra, was identified (1). This Type I receptor complex appears to mediate all the known IL-1 biological responses. The receptor Type II has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the Type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling Type I receptor complex. Recombinant IL-1 soluble receptor Type I is a potent antagonist of IL-1 action.

  1. Greenfeder, S. et al. (1995) J. Biol. Chem. 270:13757.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

7270-IL/CF
Species: Hu
NBP1-48320
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-80905
Species: Hu
Applications: ICC/IF, IHC, IHC-P
H00010963-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
NBP2-02148
Species: Hu, Mk
Applications: WB, Flow, ICC/IF
NBP1-23000
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP1-90149
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NB100-56583
Species: Hu, Rb
Applications: WB, Flow, IHC-P
NBP1-84550
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-15939
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IP
H00003845-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IP
AF6696
Species: Hu, Mu, Rt
Applications: WB, ICC
AF269
Species: Hu
Applications: WB, Flow, B/N, CyTOF-ready

Publications for IL-1 RI Antibody (AF269)(7)

We have publications tested in 2 confirmed species: Human, Amphibian.

We have publications tested in 6 applications: Blocking, ELISA Development, Flow, ICC Fluorescence, Neut, WB.


Filter By Application
Blocking
(1)
ELISA Development
(1)
Flow
(2)
ICC Fluorescence
(1)
Neut
(1)
WB
(1)
All Applications
Filter By Species
Human
(6)
Amphibian
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using AF269 Applications Species
Achille Anselmo Expression and function of IL-1R8 (TIR8/SIGIRR), a regulatory member of the IL-1 receptor family in platelets Cardiovasc Res, 2016;0(0):. 2016 [PMID: 27297888] (Flow,, Human) Flow Human
Mattiola I, Pesant M, Tentorio P, Molgora M, Marcenaro E, Lugli E, Locati M, Mavilio D Priming of Human Resting NK Cells by Autologous M1 Macrophages via the Engagement of IL-1beta, IFN-beta, and IL-15 Pathways. J Immunol, 2015;195(6):2818-28. 2015 [PMID: 26276870] (ICC Fluorescence,, Human) ICC Fluorescence Human
Hengartner N, Fiedler J, Schrezenmeier H, Huber-Lang M, Brenner R Crucial role of IL1beta and C3a in the in vitro-response of multipotent mesenchymal stromal cells to inflammatory mediators of polytrauma. PLoS ONE, 2015;10(1):e0116772. 2015 [PMID: 25562599] (Blocking,, Human) Blocking Human
Duhen T, Campbell D IL-1beta promotes the differentiation of polyfunctional human CCR6+CXCR3+ Th1/17 cells that are specific for pathogenic and commensal microbes. J Immunol, 2014;193(1):120-9. 2014 [PMID: 24890729] (Flow,, Human) Flow Human
Zielinski CE, Mele F, Aschenbrenner D, Jarrossay D, Ronchi F, Gattorno M, Monticelli S, Lanzavecchia A, Sallusto F Pathogen-induced human TH17 cells produce IFN-gamma or IL-10 and are regulated by IL-1beta. Nature, 2012;484(7395):514-8. 2012 [PMID: 22466287] (Neut,, Human) Neut Human
Herrmann-Lavoie C, Rao CV, Akoum A Chorionic gonadotropin down-regulates the expression of the decoy inhibitory interleukin 1 receptor type II in human endometrial epithelial cells. Endocrinology, 2007;148(11):5377-84. 2007 [PMID: 17702847] (ELISA Development,, Human) ELISA Development Human
Jantra S, Bigliardi E, Brizzi R, Ietta F, Bechi N, Paulesu L Interleukin 1 in oviductal tissues of viviparous, oviparous, and ovuliparous species of amphibians. Biol. Reprod., 2007;76(6):1009-15. 2007 [PMID: 17314310] (WB,, Amphibian) WB Amphibian

Reviews for IL-1 RI Antibody (AF269) (0)

There are no reviews for IL-1 RI Antibody (AF269). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IL-1 RI Antibody (AF269) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for IL-1 RI Antibody (AF269)

Discover related pathways, diseases and genes to IL-1 RI Antibody (AF269). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IL-1 RI Antibody (AF269)

Discover more about diseases related to IL-1 RI Antibody (AF269).
 

Pathways for IL-1 RI Antibody (AF269)

View related products by pathway.

PTMs for IL-1 RI Antibody (AF269)

Learn more about PTMs related to IL-1 RI Antibody (AF269).

Blogs on IL-1 RI

There are no specific blogs for IL-1 RI, but you can read our latest blog posts.

Customers Who Bought This Also Bought

IL-1 RI
269-1R-100/CF

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our IL-1 RI Antibody [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol IL1R1