IL-1 RI Antibody [Unconjugated]

AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." > AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." title="HUVEC human umbilical vein endothelial cells were labeled with Human IL-1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF269, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." />

HUVEC human umbilical vein endothelial cells were labeled with Human IL-1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF269, filled histogram) or control antibody (Catalog # <a class=

201-LB) induces CXCL1/GROa secretion in the HT-29 human colon adenocarcinoma cell line in a dose-dependent manner (orange line),as measured by the Human CXCL1/GROa DuoSet (Catalog # DY275). CXCL1/GROa secretion elicited by Recombinant Human IL-1 beta /IL-1F2 is neutralized (green line) by increasing concentrations of Human IL-1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF269). The ND₅₀ is typically < 5 µg/mL in the presence of 400 pg/mL Recombinant Human IL-1 beta /IL-1F2." > 201-LB) induces CXCL1/GROa secretion in the HT-29 human colon adenocarcinoma cell line in a dose-dependent manner (orange line),as measured by the Human CXCL1/GROa DuoSet (Catalog # DY275). CXCL1/GROa secretion elicited by Recombinant Human IL-1 beta /IL-1F2 is neutralized (green line) by increasing concentrations of Human IL-1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF269). The ND₅₀ is typically < 5 µg/mL in the presence of 400 pg/mL Recombinant Human IL-1 beta /IL-1F2." title="Recombinant Human IL-1 beta /IL-1F2 (Catalog # 201-LB) induces CXCL1/GROa secretion in the HT-29 human colon adenocarcinoma cell line in a dose-dependent manner (orange line),as measured by the Human CXCL1/GROa DuoSet (Catalog # DY275). CXCL1/GROa secretion elicited by Recombinant Human IL-1 beta /IL-1F2 is neutralized (green line) by increasing concentrations of Human IL-1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF269). The ND₅₀ is typically < 5 µg/mL in the presence of 400 pg/mL Recombinant Human IL-1 beta /IL-1F2." />

Recombinant Human IL-1 beta /IL-1F2 (Catalog # <a class=

Expression of IL1RI in native MSC and inhibition of the IL1beta mediated regulation of gene expression in PTC or IL1beta stimulated MSC.A) MSC on glass slides were stained with an anti-IL1RI antibody (left) or the non-specific IgG isotype control (right). Gene expression levels of B) PTGES, C) TSG6 and F) MMP1 were measured with quantitative real-time PCR and normalized to those of HPRT1 following total RNA isolation 24 h post stimulation. D) PGE2 and E) TSG6 levels in supernatants were detected with the Biotrend PGE2 Enzyme Immunoassay Kit and TSG6 ELISA. MSC were seeded at 5.2*103 cells/cm2 and stimulated with the PTC or IL1beta after the anti-IL1RI antibody or the non-specific (ns) IgG isotype control had been added. Scatter plots and mean from up to 4 donors are presented. Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0116772), licensed under a CC-BY license. Not internally tested by R&D Systems.

Expression of IL1RI in native MSC and inhibition of the IL1beta mediated regulation of gene expression in PTC or IL1beta stimulated MSC.A) MSC on glass slides were stained with an anti-IL1RI antibody (left) or the non-specific IgG isotype control (right). Gene expression levels of B) PTGES, C) TSG6 and F) MMP1 were measured with quantitative real-time PCR and normalized to those of HPRT1 following total RNA isolation 24 h post stimulation. D) PGE2 and E) TSG6 levels in supernatants were detected with the Biotrend PGE2 Enzyme Immunoassay Kit and TSG6 ELISA. MSC were seeded at 5.2*103 cells/cm2 and stimulated with the PTC or IL1beta after the anti-IL1RI antibody or the non-specific (ns) IgG isotype control had been added. Scatter plots and mean from up to 4 donors are presented. Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0116772), licensed under a CC-BY license. Not internally tested by R&D Systems.

269-1R) was serially diluted and captured by Mouse Anti-Human IL‑1 RI Monoclonal Antibody (Catalog # MAB269) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF269) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" > 269-1R) was serially diluted and captured by Mouse Anti-Human IL‑1 RI Monoclonal Antibody (Catalog # MAB269) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF269) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" title="Recombinant Human IL‑1 RI (Catalog # 269-1R) was serially diluted and captured by Mouse Anti-Human IL‑1 RI Monoclonal Antibody (Catalog # MAB269) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF269) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" />

Recombinant Human IL‑1 RI (Catalog # <a class=

• Applications: WB, Flow, B/N, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-1 RI Antibody [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-1 RI
• Specificity: Detects human IL-1 RI in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL1R1
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Neutralization
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1 RI Antibody [Unconjugated]

• CD121 antigen-like family member A
• CD121a antigen
• CD121a
• IL-1 RI
• IL1R
• IL1R1
• IL1RI
• IL-1RI
• IL1RT1
• IL-1RT1
• IL-1RT-1
• Interleukin 1 Receptor 1
• interleukin 1 receptor, type I
• interleukin receptor 1
• interleukin-1 receptor type 1
• p80

Background

Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor Type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts, and endothelial cells. IL-1 receptor Type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence identity in their extracellular domains. The two receptor types do not heterodimerize into a receptor complex.

An IL-1 receptor accessory protein that can heterodimerize with the Type I receptor in the presence of IL-1 alpha or IL-1 beta but not IL-1ra, was identified (1). This Type I receptor complex appears to mediate all the known IL-1 biological responses. The receptor Type II has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the Type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling Type I receptor complex. Recombinant IL-1 soluble receptor Type I is a potent antagonist of IL-1 action.

1. Greenfeder, S. et al. (1995) J. Biol. Chem. 270:13757.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-1 RI Antibody (AF269)(13)

Publications using AF269

• Giuliani, KTK;Nag, P;Adams, BC;Wang, X;Hong, S;Grivei, A;Johnston, RL;Waddell, N;Ho, KKC;Tian, Y;Khan, MA;Kim, CS;Ng, MSY;Gobe, G;Ungerer, JPJ;Forbes, JM;Healy, HG;Kassianos, AJ; Human proximal tubular epithelial cell interleukin-1 receptor signalling triggers G2/M arrest and cellular senescence during hypoxic kidney injury Cell death & disease 2025-01-31 [PMID: 39890773] (Immunohistochemistry, Human)
• RJ Kishton, SJ Patel, AE Decker, SK Vodnala, M Cam, TN Yamamoto, Y Patel, M Sukumar, Z Yu, M Ji, AN Henning, D Gurusamy, DC Palmer, RA Stefanescu, AT Girvin, W Lo, A Pasetto, P Malekzadeh, DC Deniger, KC Wood, NE Sanjana, NP Restifo Cancer genes disfavoring T�cell immunity identified via integrated systems approach Cell Reports, 2022-08-02;40(5):111153. 2022-08-02 [PMID: 35926468] (Neutralization, Human)
• Raphaël Jeger-Madiot, Romain Vaineau, Maud Heredia, Nicolas Tchitchek, Lisa Bertrand, Mathias Pereira et al. Naive and memory CD4+ T cell subsets can contribute to the generation of human Tfh cells iScience 2022-01-21 [PMID: 34984326]
• Lauren D. Aarreberg, Katharina Esser-Nobis, Connor Driscoll, Andrey Shuvarikov, Justin A. Roby, Michael Gale Interleukin-1 beta Induces mtDNA Release to Activate Innate Immune Signaling via cGAS-STING Molecular Cell 2019-05-01 [PMID: 30952515]
• Achille Anselmo Expression and function of IL-1R8 (TIR8/SIGIRR), a regulatory member of the IL-1 receptor family in platelets Cardiovasc Res, 2016-06-13;0(0):. 2016-06-13 [PMID: 27297888] (Flow Cytometry, Human)
• Mattiola I, Pesant M, Tentorio P, Molgora M, Marcenaro E, Lugli E, Locati M, Mavilio D Priming of Human Resting NK Cells by Autologous M1 Macrophages via the Engagement of IL-1beta, IFN-beta, and IL-15 Pathways. J Immunol, 2015-08-14;195(6):2818-28. 2015-08-14 [PMID: 26276870] (ICC, Human)
• Hengartner N, Fiedler J, Schrezenmeier H, Huber-Lang M, Brenner R Crucial role of IL1beta and C3a in the in vitro-response of multipotent mesenchymal stromal cells to inflammatory mediators of polytrauma. PLoS ONE, 2015-01-06;10(1):e0116772. 2015-01-06 [PMID: 25562599] (Blocking, ICC, Human)
• Duhen T, Campbell D IL-1beta promotes the differentiation of polyfunctional human CCR6+CXCR3+ Th1/17 cells that are specific for pathogenic and commensal microbes. J Immunol, 2014-06-02;193(1):120-9. 2014-06-02 [PMID: 24890729] (Flow Cytometry, Human)
• Jürgen Pollheimer, Johanna Bodin, Olav Sundnes, Reidunn J. Edelmann, Sigrid S. Skånland, Jon Sponheim et al. Interleukin-33 Drives a Proinflammatory Endothelial Activation That Selectively Targets Nonquiescent Cells Arteriosclerosis, Thrombosis, and Vascular Biology 2013-02-01 [PMID: 23162017]
• Zielinski CE, Mele F, Aschenbrenner D, Jarrossay D, Ronchi F, Gattorno M, Monticelli S, Lanzavecchia A, Sallusto F Pathogen-induced human TH17 cells produce IFN-gamma or IL-10 and are regulated by IL-1beta. Nature, 2012-04-26;484(7395):514-8. 2012-04-26 [PMID: 22466287] (Neutralization, Human)
• Florina Olaru, Liselotte E. Jensen Staphylococcus aureus stimulates neutrophil targeting chemokine expression in keratinocytes through an autocrine IL-1alpha signaling loop Journal of Investigative Dermatology 2010-07-01 [PMID: 20182449] (Neutralization, Human)
• Herrmann-Lavoie C, Rao CV, Akoum A Chorionic gonadotropin down-regulates the expression of the decoy inhibitory interleukin 1 receptor type II in human endometrial epithelial cells. Endocrinology, 2007-08-16;148(11):5377-84. 2007-08-16 [PMID: 17702847] (ELISA Development, Western Blot, Human)
• Jantra S, Bigliardi E, Brizzi R, Ietta F, Bechi N, Paulesu L Interleukin 1 in oviductal tissues of viviparous, oviparous, and ovuliparous species of amphibians. Biol. Reprod., 2007-02-21;76(6):1009-15. 2007-02-21 [PMID: 17314310] (IHC-P, Western Blot, Amphibian)

Bioinformatics

• Gene Symbol: IL1R1