IL-1 RI Antibody [Unconjugated]

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Recombinant Human IL‑1 beta /IL‑1F2 (Catalog # 201-LB) induces CXCL1/GRO alpha secretion in the HT-29 humancolon adenocarcinoma cell line ina dose-dependent manner(orange line),as measured by theHuman CXCL1/GRO ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, B/N, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

IL-1 RI Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-1 RI
Specificity
Detects human IL-1 RI in direct ELISAs and Western blots.
Details of Functionality
ActivityAssaywCitation not found None None None
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IL1R1
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready
  • Neutralization
Control
IL-1 RI Knockout HeLa Cell Lysate
Publications
Read Publications using
AF269 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1 RI Antibody [Unconjugated]

  • CD121 antigen-like family member A
  • CD121a antigen
  • CD121a
  • IL-1 RI
  • IL1R
  • IL1R1
  • IL1RI
  • IL-1RI
  • IL1RT1
  • IL-1RT1
  • IL-1RT-1
  • Interleukin 1 Receptor 1
  • interleukin 1 receptor, type I
  • interleukin receptor 1
  • interleukin-1 receptor type 1
  • p80

Background

Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor Type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts, and endothelial cells. IL-1 receptor Type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence identity in their extracellular domains. The two receptor types do not heterodimerize into a receptor complex.

An IL-1 receptor accessory protein that can heterodimerize with the Type I receptor in the presence of IL-1 alpha or IL-1 beta but not IL-1ra, was identified (1). This Type I receptor complex appears to mediate all the known IL-1 biological responses. The receptor Type II has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the Type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling Type I receptor complex. Recombinant IL-1 soluble receptor Type I is a potent antagonist of IL-1 action.

  1. Greenfeder, S. et al. (1995) J. Biol. Chem. 270:13757.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-1 RI Antibody (AF269)(7)

We have publications tested in 2 confirmed species: Human, Amphibian.

We have publications tested in 6 applications: Blocking, ELISA Development, Flow Cytometry, ICC, Neutralization, Western Blot.


Filter By Application
Blocking
(1)
ELISA Development
(1)
Flow Cytometry
(2)
ICC
(1)
Neutralization
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(6)
Amphibian
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using AF269 Applications Species
Achille Anselmo Expression and function of IL-1R8 (TIR8/SIGIRR), a regulatory member of the IL-1 receptor family in platelets Cardiovasc Res, 2016;0(0):. 2016 [PMID: 27297888] (Flow Cytometry, Human) Flow Cytometry Human
Mattiola I, Pesant M, Tentorio P, Molgora M, Marcenaro E, Lugli E, Locati M, Mavilio D Priming of Human Resting NK Cells by Autologous M1 Macrophages via the Engagement of IL-1beta, IFN-beta, and IL-15 Pathways. J Immunol, 2015;195(6):2818-28. 2015 [PMID: 26276870] (ICC, Human) ICC Human
Hengartner N, Fiedler J, Schrezenmeier H, Huber-Lang M, Brenner R Crucial role of IL1beta and C3a in the in vitro-response of multipotent mesenchymal stromal cells to inflammatory mediators of polytrauma. PLoS ONE, 2015;10(1):e0116772. 2015 [PMID: 25562599] (Blocking, Human) Blocking Human
Duhen T, Campbell D IL-1beta promotes the differentiation of polyfunctional human CCR6+CXCR3+ Th1/17 cells that are specific for pathogenic and commensal microbes. J Immunol, 2014;193(1):120-9. 2014 [PMID: 24890729] (Flow Cytometry, Human) Flow Cytometry Human
Zielinski CE, Mele F, Aschenbrenner D, Jarrossay D, Ronchi F, Gattorno M, Monticelli S, Lanzavecchia A, Sallusto F Pathogen-induced human TH17 cells produce IFN-gamma or IL-10 and are regulated by IL-1beta. Nature, 2012;484(7395):514-8. 2012 [PMID: 22466287] (Neutralization, Human) Neutralization Human
Herrmann-Lavoie C, Rao CV, Akoum A Chorionic gonadotropin down-regulates the expression of the decoy inhibitory interleukin 1 receptor type II in human endometrial epithelial cells. Endocrinology, 2007;148(11):5377-84. 2007 [PMID: 17702847] (ELISA Development, Human) ELISA Development Human
Jantra S, Bigliardi E, Brizzi R, Ietta F, Bechi N, Paulesu L Interleukin 1 in oviductal tissues of viviparous, oviparous, and ovuliparous species of amphibians. Biol. Reprod., 2007;76(6):1009-15. 2007 [PMID: 17314310] (Western Blot, Amphibian) Western Blot Amphibian

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 700 FAB269N
Alexa Fluor 700 FAB269N
Allophycocyanin FAB269A
Allophycocyanin FAB269A
Biotin BAF269
Fluorescein FAB269F
Fluorescein FAB269F
Phycoerythrin FAB269P
Phycoerythrin FAB269P

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Bioinformatics

Gene Symbol IL1R1