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IgJ Antibody

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Western Blot: IgJ Antibody [H00003512-B01P] - Analysis of IGJ expression in human colon.
Western Blot: IgJ Antibody [H00003512-B01P] - Analysis of IGJ expression in human colon.
Western Blot: IgJ Antibody [H00003512-B01P] - Analysis of IGJ expression in transfected 293T cell line by IGJ polyclonal antibody. Lane 1: IGJ transfected lysate(17.49 KDa). Lane 2: Non-transfected lysate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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IgJ Antibody Summary

Immunogen
IGJ (NP_653247.1, 1 a.a. - 159 a.a.) full-length human protein. MKNHLLFWGVLAVFIKAVHVKAQEDERIVLVDNKCKCARITSRIIRSSEDPNEDIVERNIRIIVPLNNRENISDPTSPLRTRFVYHLSDLCKKCDPTEVELDNQIVTATQSNICDEDSATETCYTYDRNKCYTAVVPLVYGGETKMVETALTPDACYPD
Specificity
IGJ - immunoglobulin J polypeptide, linker protein for immunoglobulin alpha and mu polypeptides,
Isotype
IgG
Clonality
Polyclonal
Host
Mouse
Gene
JCHAIN
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A purified

Notes

Quality control test: Antibody reactive against mammalian transfected lysate.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for IgJ Antibody

  • IGCJJCH
  • immunoglobulin J chain
  • immunoglobulin J polypeptide, linker protein for immunoglobulin alpha and mupolypeptides

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Pax5/BSAP Antibody [NBP2-29905] - Analysis of 75ug of whole cell lysates from PAX5-expressing B cell lymphoma-derived cell lines, negative control Jurkat cells, and 10ul of PageRuler Plus Prestained Protein Ladder.
Immunocytochemistry/Immunofluorescence: Pax5/BSAP Antibody [NBP2-29905] - Analysis of PAX5 (green) in Ramos and negative control Jurkat cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 2% Blocker BSA for 15 minutes at room temperature. Cells were probed with a PAX5 polyclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:200 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight-554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.
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NBP2-66881
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233-FB/CF
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Immunocytochemistry/Immunofluorescence: Glutamine Synthetase Antibody [NB110-41404] - Immunofluorescence using NB110-41404. Submitted via verified customer review.
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Western Blot: MTTP Antibody [NBP1-62489] - HepG2 cell lysate.
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[Unconjugated]
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SDS-Page: Human FGF-13 Protein [NBP2-35009]
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Immunocytochemistry/Immunofluorescence: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-BLIMP1 (3H2-E8) at 10 ug/ml overnight at 4C and detected with an anti-mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Analysis using paraffin-embedded human tonsil tissue with BLIMP1/PRDM1 antibody at dilution of 1:50. Detection is completed through VC001 (DAB) and Counterstained by hematoxylin; labeling is predominantly in germinal centers. Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.
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IgJ Antibody
H00003512-B01P
IgJ Antibody
Species: Hu
Applications: WB

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Bioinformatics

Gene Symbol JCHAIN
Entrez
Uniprot