pIgR Antibody [Unconjugated]

Images

 
Western blot shows lysates of mouse liver tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse pIgR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2800) followed by HRP-conjugated Anti-Goat ...read more
pIgR was detected in immersion fixed frozen sections of mouse liver using Goat Anti-Mouse pIgR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2800) at 1 µg/mL overnight at 4 °C. Tissue was stained ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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pIgR Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse pIgR
Lys19-Lys645
Accession # O70570
Specificity
Detects mouse pIgR in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human pIgR is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PIGR
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Blockade of Receptor-ligand Interaction
Reviewed Applications
Read 1 Review rated 3
using
AF2800 in the following applications:

Publications
Read Publications using
AF2800 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for pIgR Antibody [Unconjugated]

  • FLJ22667
  • hepatocellular carcinoma associated protein TB6
  • Hepatocellular carcinoma-associated protein TB6
  • MGC125361
  • MGC125362
  • pIgR
  • Poly-Ig receptor
  • polymeric immunoglobulin receptor

Background

The mouse polymeric immunoglobulin receptor (plgR; also known as membrane secretory component) is a 115 kDa type I transmembrane glycoprotein that is synthesized as a 771 amino acid (aa) precursor. It includes an 18 aa signal sequence, a 627 aa extracellular domain (ECD) (aa 19‑645), a 23 aa transmembrane segment (aa 646‑668), and a 143 aa cytoplasmic region (aa 669‑771) (1‑3). The ECD consists of five V-type Ig-like domains and a sixth non-Ig domain that connects to the transmembrane region. The ECD of mouse plgR is 65%, 69%, 85%, 62% and 62% aa identical to the equivalent region in human, porcine, rat, bovine and canine, respectively. plgR is expressed on secretory epithelial cells and serves as a carrier that transports IgA and IgM across epithelium (1, 2, 4). On the basolateral surface of epithelial cells, the receptor initially binds non-covalently to IgA via domains #1 and #5 of the plgR. A rearrangement then occurs where a disulfide bond forms between domain #5 of the plgR and an IgA heavy chain (2). This complex is then internalized and transcytosed to the apical surface. A soluble covalent complex called secretory IgA (SIgA) is generated by proteolytic cleavage of the complex in the sixth extracellular domain of plgR and released into the lumen (5). This proteolytically generated plgR fragment is referred to as secretory component (SC). Notably, in human, plgR transcytoses constitutively, with or without ligand, creating both a bound and free, 78 kDa SC following cleavage (3). In mouse, this event would be expected to generate a 95 kDa fragment (1). The receptor component of the complex anchors the SIgA molecule to mucous (6), where it serves to protect mucous membranes that form a barrier between the interior of the body and the external environment (7).

  1. de Groot, N. et al. (1999) Transgenic Res. 8:125.
  2. Piskurich, J. et al. (1995) J. Immunol. 154:1735.
  3. Brandtzaeg, P. and F-E. Johansen (2001) Trends Immunol. 22:545.
  4. Ben-Hur, H. et al. (2004) Int. J. Mol. Med. 14:35.
  5. Asano, M. et al. (2004) Immunology 112:583.
  6. Phalipon, A. and B. Corthesy (2003) Trends Immunol. 24:55.
  7. Uren, T. et al. (2003) J. Immunol. 170:2531.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for pIgR Antibody (AF2800)(12)

We have publications tested in 2 confirmed species: Mouse, Plant.

We have publications tested in 7 applications: ELISA Capture, ELISA Development, ICC, IHC, IHC Paraffin-embedded, IHC-P, WB.


Filter By Application
ELISA Capture
(1)
ELISA Development
(2)
ICC
(1)
IHC
(1)
IHC Paraffin-embedded
(1)
IHC-P
(1)
WB
(3)
All Applications
Filter By Species
Mouse
(10)
Plant
(1)
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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using AF2800 Applications Species
M Basic, M Buettner, LM Keubler, A Smoczek, I Bruesch, S Buchheiste, A Bleich Loss of CD14 leads to disturbed epithelial-B cell crosstalk and impairment of the intestinal barrier after E. coli Nissle monoassociation Sci Rep, 2018;8(1):719. 2018 [PMID: 29335601] (IHC-P,, Mouse) IHC-P Mouse
KM Azzam, JH Madenspach, DW Cain, L Lai, KM Gowdy, P Rai, K Janardhan, N Clayton, W Cunningham, H Jensen, PS Patel, JF Kearney, GA Taylor, MB Fessler Irgm1 coordinately regulates autoimmunity and host defense at select mucosal surfaces JCI Insight, 2017;2(16):. 2017 [PMID: 28814662] (Mouse) Mouse
K Nakanishi, S Morikane, S Ichikawa, K Kurohane, Y Niwa, Y Akimoto, S Matsubara, H Kawakami, H Kobayashi, Y Imai Protection of Human Colon Cells from Shiga Toxin by Plant-based Recombinant Secretory IgA Sci Rep, 2017;7(0):45843. 2017 [PMID: 28368034] (ELISA Capture,, Plant) ELISA Capture Plant
Olsan E, Matsushita T, Rezaei M, Weimbs T Exploitation of the Polymeric Immunoglobulin Receptor for Antibody Targeting to Renal Cyst Lumens in Polycystic Kidney Disease. J Biol Chem, 2015;290(25):15679-86. 2015 [PMID: 25922073]
Moon C, Baldridge M, Wallace M, Burnham C, Virgin H, Stappenbeck T Vertically transmitted faecal IgA levels determine extra-chromosomal phenotypic variation. Nature, 2015;521(7550):90-3. 2015 [PMID: 25686606] (WB,, Mouse) WB Mouse
Moon C, VanDussen K, Miyoshi H, Stappenbeck T Development of a primary mouse intestinal epithelial cell monolayer culture system to evaluate factors that modulate IgA transcytosis. Mucosal Immunol, 2014;7(4):818-28. 2014 [PMID: 24220295] (IHC,, Mouse) IHC Mouse
Hegab, Ahmed E, Ha, Vi Luan, Bisht, Bharti, Darmawan, Daphne O, Ooi, Aik T, Zhang, Kelvin X, Paul, Manash K, Kim, Yeon Sun, Gilbert, Jennifer, Attiga, Yasser S, Alva-Ornelas, Jackelyn, Nickerson, Derek W, Gomperts, Brigitte Aldehyde dehydrogenase activity enriches for proximal airway basal stem cells and promotes their proliferation. Stem Cells Dev, 2014;23(6):664-75. 2014 [PMID: 24171691] (ICC,, Mouse) ICC Mouse
Louten J, Mattson JD, Malinao MC, Li Y, Emson C, Vega F, Wardle RL, Van Scott MR, Fick RB, McClanahan TK, de Waal Malefyt R, Beaumont M Biomarkers of disease and treatment in murine and cynomolgus models of chronic asthma. Biomark Insights, 2012;7(0):87-104. 2012 [PMID: 22837640] (WB,, Mouse) WB Mouse
Frantz A, Rogier E, Weber C, Shen L, Cohen D, Fenton L, Bruno M, Kaetzel C Targeted deletion of MyD88 in intestinal epithelial cells results in compromised antibacterial immunity associated with downregulation of polymeric immunoglobulin receptor, mucin-2, and antibacterial peptides. Mucosal Immunol, 2012;5(5):501-12. 2012 [PMID: 22491177] (IHC Paraffin-embedded,, Mouse) IHC Paraffin-embedded Mouse
Shim DH, Ko HJ, Volker G, Potter AA, Mutwiri G, Babiuk LA, Kweon MN Efficacy of poly[di(sodium carboxylatophenoxy)phosphazene] (PCPP) as mucosal adjuvant to induce protective immunity against respiratory pathogens. Vaccine, 2010;28(11):2311-7. 2010 [PMID: 20060944] (ELISA Development,, Mouse) ELISA Development Mouse
Show All 12 Publications.

Review for pIgR Antibody (AF2800) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF2800:
Filter by Applications
IHC-Fr
(1)
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Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IHC-Fr Mouse 01/07/2015
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSee PMID 23021255
SpeciesMouse

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Recent Reviews

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Anonymous
01/07/2015
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol PIGR
Entrez
Uniprot