pIgR Antibody [Unconjugated]

Western blot shows lysates of mouse liver tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse pIgR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2800) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for pIgR at approximately 85 to 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

pIgR was detected in immersion fixed frozen sections of mouse liver using Goat Anti-Mouse pIgR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2800) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to hepatocyte cytoplasm and plasma membrane. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

pIgR Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse pIgR
  Lys19-Lys645
  Accession # O70570
• Specificity: Detects mouse pIgR in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human pIgR is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PIGR
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for pIgR Antibody [Unconjugated]

• FLJ22667
• hepatocellular carcinoma associated protein TB6
• Hepatocellular carcinoma-associated protein TB6
• MGC125361
• MGC125362
• pIgR
• Poly-Ig receptor
• polymeric immunoglobulin receptor

Background

The mouse polymeric immunoglobulin receptor (plgR; also known as membrane secretory component) is a 115 kDa type I transmembrane glycoprotein that is synthesized as a 771 amino acid (aa) precursor. It includes an 18 aa signal sequence, a 627 aa extracellular domain (ECD) (aa 19‑645), a 23 aa transmembrane segment (aa 646‑668), and a 143 aa cytoplasmic region (aa 669‑771) (1‑3). The ECD consists of five V-type Ig-like domains and a sixth non-Ig domain that connects to the transmembrane region. The ECD of mouse plgR is 65%, 69%, 85%, 62% and 62% aa identical to the equivalent region in human, porcine, rat, bovine and canine, respectively. plgR is expressed on secretory epithelial cells and serves as a carrier that transports IgA and IgM across epithelium (1, 2, 4). On the basolateral surface of epithelial cells, the receptor initially binds non-covalently to IgA via domains #1 and #5 of the plgR. A rearrangement then occurs where a disulfide bond forms between domain #5 of the plgR and an IgA heavy chain (2). This complex is then internalized and transcytosed to the apical surface. A soluble covalent complex called secretory IgA (SIgA) is generated by proteolytic cleavage of the complex in the sixth extracellular domain of plgR and released into the lumen (5). This proteolytically generated plgR fragment is referred to as secretory component (SC). Notably, in human, plgR transcytoses constitutively, with or without ligand, creating both a bound and free, 78 kDa SC following cleavage (3). In mouse, this event would be expected to generate a 95 kDa fragment (1). The receptor component of the complex anchors the SIgA molecule to mucous (6), where it serves to protect mucous membranes that form a barrier between the interior of the body and the external environment (7).

1. de Groot, N. et al. (1999) Transgenic Res. 8:125.
2. Piskurich, J. et al. (1995) J. Immunol. 154:1735.
3. Brandtzaeg, P. and F-E. Johansen (2001) Trends Immunol. 22:545.
4. Ben-Hur, H. et al. (2004) Int. J. Mol. Med. 14:35.
5. Asano, M. et al. (2004) Immunology 112:583.
6. Phalipon, A. and B. Corthesy (2003) Trends Immunol. 24:55.
7. Uren, T. et al. (2003) J. Immunol. 170:2531.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for pIgR Antibody (AF2800)(23)

Publications using AF2800

• Donald, K;Serapio-Palacios, A;Gerbec, Z;Bozorgmehr, T;Holani, R;Cruz, AR;Schnupf, P;Finlay, BB; Secretory IgA in breast milk protects against asthma through modulation of the gut microbiota Cell reports 2024-10-04 [PMID: 39368092] (ELISA Capture, Transgenic Mouse)
• Kinashi, Y;Tanaka, K;Kimura, S;Hirota, M;Komiyama, S;Shindo, T;Hashiguchi, A;Takahashi, D;Shibata, S;Karaki, SI;Ohno, H;Hase, K; Intestinal epithelium dysfunctions cause IgA deposition in the kidney glomeruli of intestine-specific Ap1m2-deficient mice EBioMedicine 2024-07-25 [PMID: 39059316] (IHC Paraffin-embedded, Mouse)
• Shikai Hu, Silvia Liu, Yu Bian, Minakshi Poddar, Sucha Singh, Catherine Cao et al. Single-cell spatial transcriptomics reveals a dynamic control of metabolic zonation and liver regeneration by endothelial cell Wnt2 and Wnt9b Cell Reports Medicine 2022-10-10 [PMID: 36220068]
• Bin Zeng, Hailong Wang, Junyi Luo, Meiying Xie, Zhengjiang Zhao, Xingping Chen et al. Porcine Milk-Derived Small Extracellular Vesicles Promote Intestinal Immunoglobulin Production through pIgR Animals (Basel) 5/24/2021 [PMID: 34073819]
• AM Collin, M Lecocq, S Noel, B Detry, FM Carlier, F Aboubakar, C Bouzin, T Leal, M Vermeersch, V De Rose, L Regard, C Martin, PR Burgel, D Hoton, S Verleden, A Froidure, C Pilette, S Gohy Lung immunoglobulin A immunity dysregulation in cystic fibrosis EBioMedicine, 2020-09-11;60(0):102974. 2020-09-11 [PMID: 32927272] (IHC, Human)
• B Zeng, D Wang, H Wang, T Chen, J Luo, Q Xi, J Sun, Y Zhang Dietary Soy Protein Isolate Attenuates Intestinal Immunoglobulin and Mucin Expression in Young Mice Compared with Casein Nutrients, 2020-09-08;12(9):. 2020-09-08 [PMID: 32911830] (Western Blot, Mouse)
• Chao Yang, Ilaria Mogno, Eduardo J. Contijoch, Joshua N. Borgerding, Varun Aggarwala, Zhihua Li et al. Fecal IgA Levels Are Determined by Strain-Level Differences in Bacteroides ovatus and Are Modifiable by Gut Microbiota Manipulation Cell Host & Microbe 2020-03-11 [PMID: 32075742]
• Noval Rivas M, Wakita D, Franklin MK et al. Intestinal Permeability and IgA Provoke Immune Vasculitis Linked to Cardiovascular Inflammation Immunity 9/17/2019 [PMID: 31471109]
• C Bornstein, S Nevo, A Giladi, N Kadouri, M Pouzolles, F Gerbe, E David, A Machado, A Chuprin, B Tóth, O Goldberg, S Itzkovitz, N Taylor, P Jay, VS Zimmermann, J Abramson, I Amit Single-cell mapping of the thymic stroma identifies IL-25-producing tuft epithelial cells Nature, 2018-07-18;559(7715):622-626. 2018-07-18 [PMID: 30022162] (IHC, Mouse)
• M Basic, M Buettner, LM Keubler, A Smoczek, I Bruesch, S Buchheiste, A Bleich Loss of CD14 leads to disturbed epithelial-B cell crosstalk and impairment of the intestinal barrier after E. coli Nissle monoassociation Sci Rep, 2018-01-15;8(1):719. 2018-01-15 [PMID: 29335601] (IHC-P, Mouse)
• KM Azzam, JH Madenspach, DW Cain, L Lai, KM Gowdy, P Rai, K Janardhan, N Clayton, W Cunningham, H Jensen, PS Patel, JF Kearney, GA Taylor, MB Fessler Irgm1 coordinately regulates autoimmunity and host defense at select mucosal surfaces JCI Insight, 2017-08-17;2(16):. 2017-08-17 [PMID: 28814662] (Western Blot, Mouse)
• K Nakanishi, S Morikane, S Ichikawa, K Kurohane, Y Niwa, Y Akimoto, S Matsubara, H Kawakami, H Kobayashi, Y Imai Protection of Human Colon Cells from Shiga Toxin by Plant-based Recombinant Secretory IgA Sci Rep, 2017-04-03;7(0):45843. 2017-04-03 [PMID: 28368034] (ELISA Development (Capture), IHC, Plant)
• Alessandra Livraghi-Butrico, Barbara R. Grubb, Kristen J. Wilkinson, Allison S. Volmer, Kimberly A. Burns, Christopher M. Evans et al. Contribution of mucus concentration and secreted mucins Muc5ac and Muc5b to the pathogenesis of muco-obstructive lung disease Mucosal Immunology 2017-03-01 [PMID: 27435107]
• Olsan E, Matsushita T, Rezaei M, Weimbs T Exploitation of the Polymeric Immunoglobulin Receptor for Antibody Targeting to Renal Cyst Lumens in Polycystic Kidney Disease. J Biol Chem, 2015-04-28;290(25):15679-86. 2015-04-28 [PMID: 25922073] (Western Blot, Mouse)
• Moon C, Baldridge M, Wallace M, Burnham C, Virgin H, Stappenbeck T Vertically transmitted faecal IgA levels determine extra-chromosomal phenotypic variation. Nature, 2015-02-16;521(7550):90-3. 2015-02-16 [PMID: 25686606] (IHC-P, Western Blot, Mouse)
• Moon C, VanDussen K, Miyoshi H, Stappenbeck T Development of a primary mouse intestinal epithelial cell monolayer culture system to evaluate factors that modulate IgA transcytosis. Mucosal Immunol, 2013-11-13;7(4):818-28. 2013-11-13 [PMID: 24220295] (IHC, Mouse)
• Hegab , Ahmed E, Ha , Vi Luan, Bisht , Bharti, Darmawan , Daphne O, Ooi , Aik T, Zhang , Kelvin X, Paul , Manash K, Kim , Yeon Sun, Gilbert , Jennifer, Attiga , Yasser S, Alva-Ornelas , Jackelyn, Nickerson , Derek W, Gomperts , Brigitte Aldehyde dehydrogenase activity enriches for proximal airway basal stem cells and promotes their proliferation. Stem Cells Dev, 2014-01-02;23(6):664-75. 2014-01-02 [PMID: 24171691] (ICC, Mouse)
• Aaron F. Heneghan, Joseph F. Pierre, Kenneth A. Kudsk IL-25 Improves IgA Levels During Parenteral Nutrition Through the JAK-STAT Pathway Annals of Surgery 2013-12-01 [PMID: 23160152] (Immunohistochemistry, Mouse)
• Louten J, Mattson JD, Malinao MC, Li Y, Emson C, Vega F, Wardle RL, Van Scott MR, Fick RB, McClanahan TK, de Waal Malefyt R, Beaumont M Biomarkers of disease and treatment in murine and cynomolgus models of chronic asthma. Biomark Insights, 2012-07-10;7(0):87-104. 2012-07-10 [PMID: 22837640] (Western Blot, Mouse)
• Frantz A, Rogier E, Weber C, Shen L, Cohen D, Fenton L, Bruno M, Kaetzel C Targeted deletion of MyD88 in intestinal epithelial cells results in compromised antibacterial immunity associated with downregulation of polymeric immunoglobulin receptor, mucin-2, and antibacterial peptides. Mucosal Immunol, 2012-04-11;5(5):501-12. 2012-04-11 [PMID: 22491177] (IHC-P, Mouse)
• Shim DH, Ko HJ, Volker G, Potter AA, Mutwiri G, Babiuk LA, Kweon MN Efficacy of poly[di(sodium carboxylatophenoxy)phosphazene] (PCPP) as mucosal adjuvant to induce protective immunity against respiratory pathogens. Vaccine, 2010-01-08;28(11):2311-7. 2010-01-08 [PMID: 20060944] (ELISA Development, Mouse)
• Libanova R, Ebensen T, Schulze K, Bruhn D, Norder M, Yevsa T, Morr M, Guzman CA The member of the cyclic di-nucleotide family bis-(3&#039;, 5&#039;)-cyclic dimeric inosine monophosphate exerts potent activity as mucosal adjuvant. Vaccine, 2010-01-07;28(10):2249-58. 2010-01-07 [PMID: 20060510] (ELISA Development, Mouse)
• Kang X, Li N, Wang M, Boontheung P, Sioutas C, Harkema JR, Bramble LA, Nel AE, Loo JA Adjuvant effects of ambient particulate matter monitored by proteomics of bronchoalveolar lavage fluid. Proteomics, 2010-02-01;10(3):520-31. 2010-02-01 [PMID: 20029843] (Western Blot, Mouse)

Review for pIgR Antibody (AF2800) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IHC-Fr Mouse 01/07/2015

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: See PMID 23021255
• Species: Mouse

Bioinformatics

• Gene Symbol: PIGR
• Uniprot:
  • O70570(Mouse)