IGFBP-3 Antibody

Immunohistochemistry-Frozen: IGFBP-3 Antibody [NBP2-16933] - Sample: Frozen sectioned E13.5 Rat brain. Green: IGFBP3 protein stained by IGFBP3 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.

Western Blot: IGFBP-3 Antibody [NBP2-16933] - U87-MG whole cell extract and conditioned medium (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with IGFBP-3 antibody [N2C3] (NBP2-16933) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunohistochemistry-Paraffin: IGFBP-3 Antibody [NBP2-16933] - IGFBP-3 antibody [N2C3] detects secreted IGFBP-3 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.IGFBP-3 stained by IGFBP-3 antibody [N2C3] (NBP2-16933) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Mouse plasma (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with IGFBP3 antibody (NBP2-16933) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

IGFBP-3 Antibody Summary

• Description: Novus Biologicals Rabbit IGFBP-3 Antibody (NBP2-16933) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human IGFBP-3. The exact sequence is proprietary.
• Localization: Secreted
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IGFBP3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 32 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Bovine, Pig, Rhesus Monkey (80%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for IGFBP-3 Antibody

• acid stable subunit of the 140 K IGF complex
• binding protein 29
• binding protein 53
• growth hormone-dependent binding protein
• IBP-3
• IBP3BP-53
• IGF-binding protein 3
• IGFBP3
• IGFBP-3
• insulin-like growth factor binding protein 3
• insulin-like growth factor-binding protein 3

Background

This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: IGFBP3
• Uniprot:
  • P17936()