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675-B3) inhibits Recombinant Human IGF‑II (Catalog # 292-G2) induced proliferation in the MCF‑7 human breast cancer cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IGF‑II (14 ng/mL) activity elicited by Recombinant Human IGFBP‑3 (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Human IGFBP‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF675). The ND50 is typically 0.75-3.0 µg/mL." class="big_lightbox" target="_blank">
675-B3) was serially diluted and captured by Mouse Anti-Human IGFBP‑3 Monoclonal Antibody (Catalog # MAB305) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IGFBP‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF675) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
675-B3) was serially diluted and captured by Mouse Anti-Human IGFBP‑3 Monoclonal Antibody (Catalog # MAB305) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IGFBP‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF675) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
HAF109). A specific band was detected for IGFBP-3 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox" target="_blank">
775-B3) was serially diluted and captured by Rat Anti-Mouse IGFBP‑3 Monoclonal Antibody (Catalog # MAB775) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IGFBP‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF775) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
IGFBP3 is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]
![Immunohistochemistry IGFBP-3 Antibody (84728) [Unconjugated]](http://images.novusbio.com/images/antibody/IGFBP-3_MAB305_Immunohistochemistry_11390.jpg)
![Western Blot IGFBP-3 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/IGFBP-3_AF775_Western_Blot_11777.jpg)
![Sandwich ELISA IGFBP-3 Antibody Pair [HRP]](http://images.novusbio.com/fullsize/IGFBP-3-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79462-img0001.jpg)
![N/A IGFBP-3 [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_IGFBP3_DGB300_ELISA_694.jpg)
![N/A IGFBP-3 [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_IGFBP3_MGB300_ELISA_981.jpg)
![Western Blot IGFBP-3 Antibody (84728) [Unconjugated]](http://images.novusbio.com/fullsize/mab305_human-igfbp-3-mab-clone-84728-4120241252141.jpg)
![Western Blot IGFBP-3 Antibody (84728) [Unconjugated]](http://images.novusbio.com/fullsize/mab305_human-igfbp-3-mab-clone-84728-22820241721187.jpg)
![Western Blot IGFBP-3 Antibody (84728) [Unconjugated]](http://images.novusbio.com/fullsize/mab305_human-igfbp-3-mab-clone-84728-western-blot-121220258452121.jpg)
![ELISA IGFBP-3 Antibody (84728) [Unconjugated]](http://images.novusbio.com/images/mab305_human-igfbp-3-mab-clone-84728-elisa-639201780596515251.jpg)
![Simple Western IGFBP-3 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/IGFBP3_AF775_Simple_Western_16936.jpg)
![ELISA IGFBP-3 Antibody [Unconjugated]](http://images.novusbio.com/images/af775_mouse-igfbp-3-affinity-purified-polyclonal-ab-elisa-639201852916741489.jpg)
![N/A IGFBP-3 [HRP]](http://images.novusbio.com/fullsize/elisa/IGFBP-3_DGB300_ELISA_104.jpg)
![N/A IGFBP-3 [HRP]](http://images.novusbio.com/fullsize/elisa/IGFBP-3_MGB300_ELISA_273.jpg)
