IGFBP-3 Antibodies

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="IGFBP‑3 was detected in immersion fixed paraffin-embedded sections of human colon using 10 µg/mL Human IGFBP‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF675) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

675-B3) inhibits Recombinant Human IGF‑II (Catalog # 292-G2) induced proliferation in the MCF‑7 human breast cancer cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IGF‑II (14 ng/mL) activity elicited by Recombinant Human IGFBP‑3 (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Human IGFBP‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF675). The ND₅₀ is typically 0.75-3.0 µg/mL." class="big_lightbox"> 675-B3) inhibits Recombinant Human IGF‑II (Catalog # 292-G2) induced proliferation in the MCF‑7 human breast cancer cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IGF‑II (14 ng/mL) activity elicited by Recombinant Human IGFBP‑3 (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Human IGFBP‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF675). The ND₅₀ is typically 0.75-3.0 µg/mL." class="big_thumb" />

675-B3) was serially diluted and captured by Mouse Anti-Human IGFBP‑3 Monoclonal Antibody (Catalog # MAB305) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IGFBP‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF675) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 675-B3) was serially diluted and captured by Mouse Anti-Human IGFBP‑3 Monoclonal Antibody (Catalog # MAB305) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IGFBP‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF675) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

CTS003) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS003) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="IGFBP-3 was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human IGFBP-3 Monoclonal Antibody (Catalog # MAB305) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

675-B3) was serially diluted and captured by Mouse Anti-Human IGFBP‑3 Monoclonal Antibody (Catalog # MAB305) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IGFBP‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF675) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 675-B3) was serially diluted and captured by Mouse Anti-Human IGFBP‑3 Monoclonal Antibody (Catalog # MAB305) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IGFBP‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF675) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

HAF109). A specific band was detected for IGFBP-3 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF109). A specific band was detected for IGFBP-3 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse IGFBP-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF775) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IGFBP-3 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox"> HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

775-B3) was serially diluted and captured by Rat Anti-Mouse IGFBP‑3 Monoclonal Antibody (Catalog # MAB775) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IGFBP‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF775) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 775-B3) was serially diluted and captured by Rat Anti-Mouse IGFBP‑3 Monoclonal Antibody (Catalog # MAB775) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IGFBP‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF775) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

775-B3) was serially diluted and captured by Rat Anti-Mouse IGFBP‑3 Monoclonal Antibody (Catalog # MAB775) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IGFBP‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF775) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 775-B3) was serially diluted and captured by Rat Anti-Mouse IGFBP‑3 Monoclonal Antibody (Catalog # MAB775) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IGFBP‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF775) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Mouse IGFBP‑3 (Catalog # 775-B3) was serially diluted and captured by Rat Anti-Mouse IGFBP‑3 Monoclonal Antibody (Catalog # MAB775) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IGFBP‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF775) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="IGFBP‑3 was detected in fixed WM-115 cells (Positive) and absent in U266 human myeloma cell line (Negative) using Mouse Anti-Human IGFBP‑3 Monoclonal Antibody (Catalog # mab11488) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />