Rat IFN-gamma ELISA Kit (Colorimetric)

ELISA: Interferon gamma ELISA Kit 96Tests [NBP1-92680]

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Cell culture supernatant, serum
• Standard Curve Range: 31.3 - 2,000 pg/mL
• Sensitivity: 9.9 pg/mL

Rat IFN-gamma ELISA Kit (Colorimetric) Summary

• Description: The rat IFN-gamma ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of rat IFN-gamma
• Specificity: The interference of circulating factors of the immune systemwas evaluated by spiking these proteins at physiologically relevant concentrations into a rat IFN gammapositive serum. There was no crossreactivity detected.
• Standard Curve Range: 31.3 - 2,000 pg/mL
• Sensitivity: 9.9 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 10%
• Intra-Assay: CV% < 5.0%
• Spike Recovery: Refer to manual
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IFNG

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat IFN-gamma ELISA Kit (Colorimetric)

• IFG
• IFI
• IFNG
• IFNgamma
• IFN-gamma
• Immune interferon
• interferon gamma
• interferon, gamma

Background

IFN-gamma, also called Type III interferon, is a homodimeric glycoprotein containing approximately 21 to 24 kD subunits. In contrast to IFN-alpha and IFN-beta synthesis, which can occur in any cell, production of IFN-gamma is a function of T cells and NK cells. All IFN-gamma inducers activate T cells either in a polyclonal (mitogens or antibodies) or in a clonally restricted, antigen-specific, manner. IFN-gamma is produced during infection by T cells of the cytotoxic/suppressor phenotype (CD8) and by a subtype of helper T cells, the Th1 cells. Th1 cells secrete IL-2, IL-3, TNF-beta and IFN-gamma, whereas Th2 cells mainly produce IL-3, IL-4, IL-5, and IL-10, but little or no IFN-gamma. IFN-gamma preferentially inhibits the proliferation of Th2 but not Th1 cells, indicating that the presence of IFN-gamma during an immune response will result in the preferential proliferation of Th1 cells. Type II IFN or IFN-gamma is a lymphokine that displays no molecular homology with type I IFN, but shares some important biologic activities. Specifically, IFN-gamma induces an anti-viral state and is anti-proliferative. In addition, IFN-gamma has several properties related to immunoregulation. (1) IFN-gamma is a potent activator of mononuclear phagocytes, augments endocytosis and phagocytosis by monocytes, and activates macrophages to kill tumor cells by releasing reactive oxygen intermediates and TNF-alpha. (2) IFN-gamma induces or augments the expression of MHC antigens on macrophages, T and B cells and some tumor cell lines. (3) On T and B cells IFN-gamma promotes differentiation. (4) Finally, IFN-gamma activates neutrophils, NK cells and vascular endothelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for IFN-gamma ELISA Kit (NBP1-92680)(2)

Publications using NBP1-92680

• Qu Z, Li M, An R et al. Self-assembled BPIV3 nanoparticles can induce comprehensive immune responses and protection against BPIV3 challenge by inducing dendritic cell maturation in mice Veterinary Microbiology 2022-03-01 [PMID: 35395543] (ELISA)
• He J, Li Z, Yu T et al. In Vitro and In Vivo biocompatibility study on acellular sheep periosteum for guided bone regeneration Biomed. Mater. 2019-11-20 [PMID: 31747647]

FAQs for IFN-gamma ELISA Kit (NBP1-92680). (Showing 1 - 1 of 1 FAQs).

1. Is Recombinant Human IFN-gamma Protein - Clinical grade or preclinical ?
   • We are research use only.

Bioinformatics

• Gene Symbol: IFNG