Mouse IFN-gamma ELISA Kit (Colorimetric)

ELISA: Mouse IFN-gamma ELISA Kit (Colorimetric) [NBP1-92669]

Product Discontinued

Mouse IFN-gamma ELISA Kit (Colorimetric) Summary

• Specificity: The interference of circulating factors of the immune systemwas evaluated by spiking these proteins at physiologically relevant concentrations into a mouse IFNgamma positive serum. There was no crossreactivity detected.
• Standard Curve Range: 15.6 - 1,000 pg/mL
• Sensitivity: 5.3 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 10%
• Intra-Assay: CV% < 5.0%
• Spike Recovery: 76%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IFNG

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: The mouse IFN-gamma ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse IFN-gamma.

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse IFN-gamma ELISA Kit (Colorimetric)

• IFG
• IFI
• IFNG
• IFNgamma
• IFN-gamma
• Immune interferon
• interferon gamma
• interferon, gamma

Background

IFN-gamma, also called Type II interferon, is a homodimeric gycoprotein containing approximately 21 to 24 kD subunits. In contrast to IFN-gamma and IFN-gamma synthesis, which can occur in any cell, production of IFN-gamma is a function of T cells and NK cells. Type II IFN or IFN-gamma is a lymphokine that displays no molecular homology with type I IFN, but shares some important biologic activities. Specifically, IFN induces an anti-viral state and is anti-proliferative. In addition, IFN-gamma has several properties related to immunoregulation. (1) IFN- is a potent activator of mononuclear phagocytes, e.g. IFN-gamma stimulates the expression of Mac-1, augments endocytosis and phagocytosis by monocytes; and activates macrophages to kill tumor cells by releasing reactive oxagen intermediates and TNF-alpha. (2) IFN-gamma induces or augments the expression of MHC antigens on macrophages, T and B cells and some tumor cell lines. (3) On T and B cells IFN- promotes differentiation. It enhaces proliferation of activated B cells and can act synergistically with IL-2 to increase immunoglobulin light-chain synthesis. IFN-gamma is one of the natural B-cell differentiation factors. (4) Finally, IFN-gamma activates neutrophils, NK cells and vascular endothelial cells. The role of IFN-gamma as a disease marker has been demonstrated for a number of different pathological situations

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for IFN-gamma ELISA Kit (NBP1-92669)(2)

Publications using NBP1-92669

• Yan J, Zhu J, Li X et al. Blocking LTB4 signaling-mediated TAMs recruitment by Rhizoma Coptidis sensitizes lung cancer to immunotherapy Phytomedicine : international journal of phytotherapy and phytopharmacology 2023-10-01 [PMID: 37531900] (ELISA)
• Zhu J, Huang R, Ya J et al. Chelerythrine and Chelidonine Decrease Extracellular Adenosine Improving Cd8 + T Anti-Tumor Immunity SSRN Electronic Journal 2021-09-23 (ELISA)

FAQs for IFN-gamma ELISA Kit (NBP1-92669). (Showing 1 - 2 of 2 FAQs).

1. Is Recombinant Human IFN-gamma Protein - Clinical grade or preclinical ?
   • We are research use only.
2. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: IFNG