IFN-gamma Antibody [Biotin]

IGFBP-1 was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) stimulated with PMA and calcium ionomycin using Goat Anti-Human IGFBP-1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF871) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Ca
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

IFN-gamma Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant canine IFN-gamma
  Gln24-Lys166
  Accession # P42161
• Specificity: Detects canine IFN-gamma in direct ELISAs and Western blots. In Western blots, approximately 25% cross-reactivity with recombinant human IFN‑ gamma is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IFNG
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IFN-gamma Antibody [Biotin]

• IFG
• IFI
• IFNG
• IFNgamma
• IFN-gamma
• Immune interferon
• interferon gamma
• interferon, gamma

Background

Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature canine IFN-gamma exists as a noncovalently linked homodimer of 20-25 kDa variably glycosylated subunits (3). It shares 79-88% amino acid sequence identity with bovine, equine, and feline IFN-gamma , 62-73% with human, porcine, and rhesus IFN-gamma , and 40-47% with cotton rat, mouse, and rat IFN-gamma . IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).

1. Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
3. Zucker, K. et al. (1992) J. Interferon Res. 12:191.
4. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. USA 92:5401.
5. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
6. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
7. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
8. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
9. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IFN-gamma Antibody (BAF781)(6)

Publications using BAF781

• M Rioux, ME Francis, CL Swan, A Ge, A Kroeker, AA Kelvin The Intersection of Age and Influenza Severity: Utility of Ferrets for Dissecting the Age-Dependent Immune Responses and Relevance to Age-Specific Vaccine Development Viruses, 2021-04-15;13(4):. 2021-04-15 [PMID: 33920917] (Flow Cytometry, Ferret)
• Won Sik Lee, Yasuhiro Suzuki, Scott S. Graves, Mineo Iwata, G.M. Venkataraman, Marco Mielcarek et al. Canine Bone Marrow Derived Mesenchymal Stromal Cells Suppress Allo-Reactive Lymphocyte Proliferation in Vitro but Fail to Enhance Engraftment in Canine Bone Marrow Transplantation Biology of Blood and Marrow Transplantation 4/1/2011 [PMID: 20457265]
• Lemesre JL, Holzmuller P, Goncalves RB, Bourdoiseau G, Hugnet C, Cavaleyra M, Papierok G Long-lasting protection against canine visceral leishmaniasis using the LiESAp-MDP vaccine in endemic areas of France: double-blind randomised efficacy field trial. Vaccine, 2007-03-15;25(21):4223-34. 2007-03-15 [PMID: 17395339] (ELISA Development, Canine)
• Fujiwara RT, Loukas A, Mendez S, Williamson AL, Bueno LL, Wang Y, Samuel A, Zhan B, Bottazzi ME, Hotez PJ, Bethony JM Vaccination with irradiated Ancylostoma caninum third stage larvae induces a Th2 protective response in dogs. Vaccine, 2005-08-15;24(4):501-9. 2005-08-15 [PMID: 16140437] (ELISA Development, Canine)
• Lemesre JL, Holzmuller P, Cavaleyra M, Goncalves RB, Hottin G, Papierok G Protection against experimental visceral leishmaniasis infection in dogs immunized with purified excreted secreted antigens of Leishmania infantum promastigotes. Vaccine, 2005-04-22;23(22):2825-40. 2005-04-22 [PMID: 15780731] (ELISA Development, Canine)
• Strauss-Ayali D, Baneth G, Shor S, Okano F, Jaffe CL Interleukin-12 augments a Th1-type immune response manifested as lymphocyte proliferation and interferon gamma production in Leishmania infantum-infected dogs. Int. J. Parasitol., 2004-12-08;35(1):63-73. 2004-12-08 [PMID: 15619517] (ELISA Development, Canine)

FAQs for IFN-gamma Antibody (BAF781). (Showing 1 - 1 of 1 FAQs).

1. Is Recombinant Human IFN-gamma Protein - Clinical grade or preclinical ?
   • We are research use only.

Bioinformatics

• Gene Symbol: IFNG
• Uniprot:
  • P42161()