IFN-gamma Antibody [Unconjugated]

IFN‑ gamma was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) untreated (lower panel) or treated with PMA and calcium ionomycin (upper panel) using Goat Anti-Canine IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF781) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Recombinant Canine IFN‑ gamma (Catalog # 781-CG) reduces the Vesticular Stomatitis Virus (VSV)-induced cytopathy in the A‑72 canine fibroma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of VSV activity elicited by Recombinant Canine IFN‑ gamma (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF781). The ND50 is typically 0.2-1 µg/mL.

• Reactivity: Ca
• Applications: WB, B/N, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IFN-gamma Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant canine IFN-gamma
  Gln24-Lys166
  Accession # P42161
• Specificity: Detects canine IFN-gamma in direct ELISAs and Western blots. In direct ELISAs, less than 40% cross-reactivity with recombinant porcine IFN‑ gamma is observed and less than 5% cross-reactivity with recombinant human IFN-gamma, recombinant rhesus macaque IFN-gamma, recombinant mouse IFN‑ gamma, and recombinant rat IFN‑ gamma is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IFNG
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
  • Immunocytochemistry 5-15 ug/mL
  • Neutralization 0.2-1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IFN-gamma Antibody [Unconjugated]

• IFG
• IFI
• IFNG
• IFNgamma
• IFN-gamma
• Immune interferon
• interferon gamma
• interferon, gamma

Background

Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature canine IFN-gamma exists as a noncovalently linked homodimer of 20‑25 kDa variably glycosylated subunits (3). It shares 79‑88% amino acid sequence identity with bovine, equine, and feline IFN-gamma, 62‑73% with human, porcine, and rhesus IFN-gamma, and 40‑47% with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).

1. Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
3. Zucker, K. et al. (1992) J. Interferon Res. 12:191.
4. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. USA 92:5401.
5. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
6. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
7. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
8. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
9. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IFN-gamma Antibody (AF781)(3)

Publications using AF781

• CG Kim, JE Lee, DG Jeong, YH Lee, SI Park, DG Lee, CH Han, SJ Kang, CH Song, SH Choi, YJ Lee, SK Ku Bathing effects of east saline groundwater concentrates on allergic (atopic) dermatitis-like skin lesions induced by 2,4-dinitrochlorobenzene in hairless mice Exp Ther Med, 2017;13(6):3448-3466. 2017 [PMID: 28587425] (IHC, Mouse)
• Mechanisms of Immune Suppression Utilized by Canine Adipose and Bone Marrow-Derived Mesenchymal Stem Cells Stem Cells Dev., 2016;0(0):. 2016 [PMID: 27881051] (Neutralization, Canine)
• Cupillard L, Juillard V, Latour S, Colombet G, Cachet N, Richard S, Blanchard S, Fischer L Impact of plasmid supercoiling on the efficacy of a rabies DNA vaccine to protect cats. Vaccine, 2005;23(16):1910-6. 2005 [PMID: 15734063] (ELISpot Development, Feline)

FAQs for IFN-gamma Antibody (AF781). (Showing 1 - 1 of 1 FAQs).

1. Is Recombinant Human IFN-gamma Protein - Clinical grade or preclinical ?
   • We are research use only.

Bioinformatics

• Gene Symbol: IFNG
• Uniprot:
  • P42161()