IFN-gamma Antibody [Unconjugated]

IFN‑ gamma was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PMA and ionomycin using 10 µg/mL Goat Anti-Human IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑285‑NA) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Recombinant Human IFN‑ gamma (Catalog # 285-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Human IFN‑ gamma (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-285-NA). The ND50 is typically 0.1‑0.3 µg/mL.

• Reactivity: Hu
• Applications: WB, B/N, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IFN-gamma Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human IFN-gamma (R&D Systems, Catalog # 285-IF)
  Met1-Gln144
  Accession # Q14609
• Specificity: Detects human IFN-gamma in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant rat IFN-gamma and recombinant mouse IFN-gamma is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IFNG
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
  • Immunocytochemistry 5-15 ug/mL
  • Neutralization 0.1-0.3 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IFN-gamma Antibody [Unconjugated]

• IFG
• IFI
• IFNG
• IFNgamma
• IFN-gamma
• Immune interferon
• interferon gamma
• interferon, gamma

Background

Interferon-gamma (IFN-gamma, IFNG), also known as type II or Immune Interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine. Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa molecular weight variably glycosylated subunits. Glycosylation of IFN-gamma at sites Asn25 and Asn97 is critical for protease resistance. It shares 90% amino acid (aa) sequence identity with rhesus IFN-gamma, 59-64% with bovine, canine, equine, feline, and porcine IFN‑ gamma, and 37-43% with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction. IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key function in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up‑regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IFN-gamma Antibody (AF-285-NA)(11)

Publications using AF-285-NA

• F Zou, L Lu, J Liu, B Xia, W Zhang, Q Hu, W Liu, Y Zhang, Y Lin, S Jing, M Huang, B Huang, B Liu, H Zhang Engineered triple inhibitory receptor resistance improves anti-tumor CAR-T cell performance via CD56 Nat Commun, 2019;10(1):4109. 2019 [PMID: 31511513] (CAR-T (IHC-Fr), Xenograft)
• Zheng J, Wu W, Liu Y, Xiang Z, Liu M, Chan K, Lau S, Lam K, To K, Chan J, Li L, Chen H, Lau Y, Yuen K, Tu W The Therapeutic Effect of Pamidronate on Lethal Avian Influenza A H7N9 Virus Infected Humanized Mice. PLoS ONE, 2015;10(8):e0135999. 2015 [PMID: 26285203] (Neutralization, Human)
• Wang R, Jaw JJ, Jaw J, Stutzman N, Stutzman NC, Zou Z, Sun PD Natural killer cell-produced IFN-gamma and TNF-alpha induce target cell cytolysis through up-regulation of ICAM-1. J Leukoc Biol, 0;91(2):299-309. 0 [PMID: 22045868] (Neutralization, Human)
• Knoblach T, Grandel B, Seiler J, Nevels M, Paulus C Human cytomegalovirus IE1 protein elicits a type II interferon-like host cell response that depends on activated STAT1 but not interferon-gamma. PLoS Pathog., 2011;7(4):e1002016. 2011 [PMID: 21533215] (Neutralization, Human)
• Mishto M, Ligorio C, Bellavista E, Martucci M, Santoro A, Giulioni M, Marucci G, Franceschi C Immunoproteasome expression is induced in mesial temporal lobe epilepsy. Biochem. Biophys. Res. Commun., 2011;408(1):65-70. 2011 [PMID: 21458417] (Western Blot, Human)
• Guo PF, Du MR, Wu HX, Lin Y, Jin LP, Li DJ Thymic stromal lymphopoietin from trophoblasts induces dendritic cell-mediated regulatory TH2 bias in the decidua during early gestation in humans. Blood, 2010;116(12):2061-9. 2010 [PMID: 20538796] (IHC, Human)
• Heo YJ, Joo YB, Oh HJ, Park MK, Heo YM, Cho ML, Kwok SK, Ju JH, Park KS, Cho SG, Park SH, Kim HY, Min JK IL-10 suppresses Th17 cells and promotes regulatory T cells in the CD4+ T cell population of rheumatoid arthritis patients. Immunol. Lett., 2010;127(2):150-6. 2010 [PMID: 19895848] (Neutralization, Human)
• Kobayashi A, Weinberg V, Darragh T, Smith-McCune K Evolving immunosuppressive microenvironment during human cervical carcinogenesis. Mucosal Immunol, 2008;1(5):412-20. 2008 [PMID: 19079205] (IHC-P, Human)
• Chi W, Zhu X, Yang P, Liu X, Lin X, Zhou H, Huang X, Kijlstra A Upregulated IL-23 and IL-17 in Behcet patients with active uveitis. Invest. Ophthalmol. Vis. Sci., 2008;49(7):3058-64. 2008 [PMID: 18579762] (Neutralization, Human)
• Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells. Eur. J. Immunol., 2008;38(4):1012-23. 2008 [PMID: 18350544] (Neutralization, Human)
• Hessian PA, Highton J, Kean A, Sun CK, Chin M Cytokine profile of the rheumatoid nodule suggests that it is a Th1 granuloma. Arthritis Rheum., 2003;48(2):334-8. 2003 [PMID: 12571841] (IHC, Human)

FAQs for IFN-gamma Antibody (AF-285-NA). (Showing 1 - 1 of 1 FAQs).

1. Is Recombinant Human IFN-gamma Protein - Clinical grade or preclinical ?
   • We are research use only.

Bioinformatics

• Gene Symbol: IFNG
• Uniprot:
  • Q14609()