IFN-gamma Antibody (D9D10) - Chimeric - Azide and BSA Free

Western Blot: IFN-gamma Antibody (D9D10) [NBP2-81044] - Western Blot using anti-IFN gamma antibody (D9D10) [NBP2-81044]. Human thymus lysate (35ug protein in RIPA buffer) was resolved on a 15% SDS PAGE gel and blots probed with the rabbit IgG-chimeric version of D9D10 [NBP2-81044] at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. Protein was detected by chemiluminescence. The expected unmodified running size for IFN gamma is 19.3kDa, though the form shown here is likely glycosylated. NBP2-81044 successfully detected IFN gamma in human thymus samples.

• Reactivity: Hu
• Applications: WB, ELISA, Flow, B/N
• Clone: D9D10
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

IFN-gamma Antibody (D9D10) - Chimeric - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit IFN-gamma Antibody (D9D10) - Chimeric - Azide and BSA Free (NBP2-81044) is a recombinant monoclonal antibody validated for use in WB, ELISA and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: FPLC-purified natural human IFN-gamma. Refer to the original article [PMID: 3114127]
• Specificity: Binds human IFN-gamma, a dimerized soluble cytokine that is the only member of the type II class of interferons.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: IFNG
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • ELISA
  • Flow Cytometry
  • Western Blot
• Application Notes: This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG1 format, for improved compatibility with existing reagents, assays and techniques. This antibody can be used to detect both native and recombinant intereferon gamma (IFNg). It is reported only to bind to the functional, dimeric form of IFNg.

Reactivity Notes

Cross Reactivity: Baboon

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for IFN-gamma Antibody (D9D10) - Chimeric - Azide and BSA Free

• IFG
• IFI
• IFNG
• IFNgamma
• IFN-gamma
• Immune interferon
• interferon gamma
• interferon, gamma

Background

IFN-gamma, a type II interferon and an acid-labile interferon, is mainly produced by CD4 and CD8 T-lymphocytes and natural killer cells activated by antigens, mitogens or alloantigens. IFN-gamma shows no significant homology with IFN-beta or IFN-alpha. IFN-gamma has antiviral and antiparasitic activities and exhibits also immunoregulatory and proinflammatory activities. IFN-gamma induces cytokine production and promotes antigen presentation by up regulating the expression of MHC proteins as well as other antigen proteins. In contrast to other interferons, which are mostly antiviral, the main activity of IFN-gamma seems to be immunomodulatory. Human IFN-gamma is species-specific and is only biologically active in human and primate cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for IFN-gamma Antibody (NBP2-81044). (Showing 1 - 1 of 1 FAQ).

1. Is Recombinant Human IFN-gamma Protein - Clinical grade or preclinical ?
   • We are research use only.

Bioinformatics

• Gene Symbol: IFNG