IFN-gamma Antibody (142529)

• Reactivity: Ca
• Applications: WB, ELISA(Cap)
• Clone: 142529
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

IFN-gamma Antibody (142529) Summary

• Immunogen: E. coli-derived recombinant canine IFN-gamma
  Gln24-Lys166
  Accession # P42161
• Specificity: Detects canine IFN-gamma in direct ELISAs and Western blots. In direct ELISAs, this antibody shows approximately 75% cross‑reactivity with recombinant porcine IFN-gamma and no cross-reactivity with IFN-gamma from human, mouse, feline, rat, or rhesus macaque.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: IFNG
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 1 ug/mL
• Application Notes: ELISA Detection: Canine IFN-gamma Biotinylated Antibody (Catalog number BAM7811)
  Standard: Recombinant Canine IFN-gamma (Catalog number 781-CG)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IFN-gamma Antibody (142529)

• IFG
• IFI
• IFNG
• IFNgamma
• IFN-gamma
• Immune interferon
• interferon gamma
• interferon, gamma

Background

Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature canine IFN-gamma exists as a noncovalently linked homodimer of 20-25 kDa variably glycosylated subunits (3). It shares 79% - 88% amino acid sequence identity with bovine, equine, and feline IFN-gamma , 62% - 73% with human, porcine, and rhesus IFN-gamma , and 40% - 47% with cotton rat, mouse, and rat IFN-gamma . IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).

1. Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
3. Zucker, K. et al. (1992) J. Interferon Res. 12:191.
4. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401.
5. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
6. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
7. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
8. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
9. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IFN-gamma Antibody (MAB781)(9)

Publications using MAB781

• BM Scorza, KG Mahachi, AD Cox, AJ Toepp, D Pessoa-Per, P Tyrrell, J Buch, JA Foltz, D Lee, CA Petersen Role of NK-Like CD8+ T Cells during Asymptomatic Borrelia burgdorferi Infection Infection and Immunity, 2022-04-13;0(0):e0055521. 2022-04-13 [PMID: 35416707] (Flow Cytometry, Canine)
• Recovery of antigen-specific T cell responses from dogs infected with Leishmania (L.) infantum by use of vaccine associated TLR-agonist adjuvant Vaccine, 2016-09-21;0(0):. 2016-09-21 [PMID: 27665354] (Flow Cytometry, Canine)
• Recombinant Forms of Leishmania amazonensis Excreted/Secreted Promastigote Surface Antigen (PSA) Induce Protective Immune Responses in Dogs PLoS Negl Trop Dis, 2016-05-25;10(5):e0004614. 2016-05-25 [PMID: 27223609] (ELISA Development (Capture), Canine)
• Esch K, Juelsgaard R, Martinez P, Jones D, Petersen C Programmed death 1-mediated T cell exhaustion during visceral leishmaniasis impairs phagocyte function. J Immunol, 2013-10-23;191(11):5542-50. 2013-10-23 [PMID: 24154626] (Flow Cytometry, Canine)
• Won Sik Lee, Yasuhiro Suzuki, Scott S. Graves, Mineo Iwata, G.M. Venkataraman, Marco Mielcarek et al. Canine Bone Marrow Derived Mesenchymal Stromal Cells Suppress Allo-Reactive Lymphocyte Proliferation in Vitro but Fail to Enhance Engraftment in Canine Bone Marrow Transplantation Biology of Blood and Marrow Transplantation 2011-04-01 [PMID: 20457265]
• Lemesre JL, Holzmuller P, Goncalves RB, Bourdoiseau G, Hugnet C, Cavaleyra M, Papierok G Long-lasting protection against canine visceral leishmaniasis using the LiESAp-MDP vaccine in endemic areas of France: double-blind randomised efficacy field trial. Vaccine, 2007-03-15;25(21):4223-34. 2007-03-15 [PMID: 17395339] (ELISA Development, Canine)
• Fujiwara RT, Loukas A, Mendez S, Williamson AL, Bueno LL, Wang Y, Samuel A, Zhan B, Bottazzi ME, Hotez PJ, Bethony JM Vaccination with irradiated Ancylostoma caninum third stage larvae induces a Th2 protective response in dogs. Vaccine, 2005-08-15;24(4):501-9. 2005-08-15 [PMID: 16140437] (ELISA Development, Canine)
• Lemesre JL, Holzmuller P, Cavaleyra M, Goncalves RB, Hottin G, Papierok G Protection against experimental visceral leishmaniasis infection in dogs immunized with purified excreted secreted antigens of Leishmania infantum promastigotes. Vaccine, 2005-04-22;23(22):2825-40. 2005-04-22 [PMID: 15780731] (ELISA Development, Canine)
• Strauss-Ayali D, Baneth G, Shor S, Okano F, Jaffe CL Interleukin-12 augments a Th1-type immune response manifested as lymphocyte proliferation and interferon gamma production in Leishmania infantum-infected dogs. Int. J. Parasitol., 2004-12-08;35(1):63-73. 2004-12-08 [PMID: 15619517] (ELISA Development, Canine)

FAQs for IFN-gamma Antibody (MAB781). (Showing 1 - 1 of 1 FAQs).

1. Is Recombinant Human IFN-gamma Protein - Clinical grade or preclinical ?
   • We are research use only.

Bioinformatics

• Gene Symbol: IFNG
• Uniprot:
  • P42161()