ICAM-1/CD54 Antibody (1H4)


Flow (Cell Surface): ICAM-1/CD54 Antibody (1H4) [NB110-58732] - Analysis using the Biotin conjugate of NB110-58732. Staining of U937 human histiocytic lymphoma cell line with anti-human CD54 (1H4) PE. Total viable cells ...read more
Flow (Cell Surface): ICAM-1/CD54 Antibody (1H4) [NB110-58732] - Analysis using the PE conjugate of NB110-58732. ICAM1 Antibody (1H4) [PE] NB500-582]-Surface staining of U937 human histiocytic lymphoma cell line with ...read more

Product Details

Reactivity HuSpecies Glossary
Applications WB, Flow, Flow-CS, IHC, IHC-Fr, IHC-P, ICC
1.0 mg/ml

ICAM-1/CD54 Antibody (1H4) Summary

Raji cells and spleen cells fused with NS1 cells.
This antibody (clone 1H4) reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus) expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells; the expression of CD54 is upregulated by activation.
Protein A purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
phosphate buffered saline (PBS), approx. pH 7.4; 0.2 um filter sterilized.
No Preservative
1.0 mg/ml
Protein A purified


Application Notes
Immunohistochemistry-Pparaffin sections recommended dilution: 10 ug/ml Application Note: Prolonged fixation in buffered formalin can destroy the epitope. Hight temperature antigen unmasking technique is required

Alternate Names for ICAM-1/CD54 Antibody (1H4)

  • BB2
  • CD54 antigen
  • CD54
  • cell surface glycoprotein P3.58
  • human rhinovirus receptor
  • ICAM1
  • ICAM-1
  • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
  • intercellular adhesion molecule 1
  • Major group rhinovirus receptor
  • P3.58


CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Allophycocyanin Labeled NB110-81732
Biotin Labeled NB500-584
FITC Labeled NB500-583
PE Labeled NB500-582

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Contact Information

Product PDFs


Gene Symbol ICAM1

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