ICAM-1/CD54 Antibody (1H4) - Azide Free Summary
Raji cells and spleen cells fused with NS1 cells.
This antibody (clone 1H4) reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus) expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells; the expression of CD54 is upregulated by activation.
Protein A purified
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- Western Blot 1:1000
- Flow Cytometry 1 ug/ml
- Immunohistochemistry 1:10-1:500
- Immunohistochemistry-Frozen 1:200
- Immunohistochemistry-Paraffin 1:200
- Flow (Cell Surface)
Immunohistochemistry-Pparaffin sections recommended dilution: 10 ug/ml Application Note: Prolonged fixation in buffered formalin can destroy the epitope. Hight temperature antigen unmasking technique is required
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
PBS approx. pH 7.4; 0.2 um filter sterilized.
Protein A purified
Alternate Names for ICAM-1/CD54 Antibody (1H4) - Azide Free
- CD54 antigen
- cell surface glycoprotein P3.58
- human rhinovirus receptor
- intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
- intercellular adhesion molecule 1
- Major group rhinovirus receptor
CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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