ICAM-1/CD54 Antibody (1H4) Summary
Raji cells and spleen cells fused with NS1 cells.
This antibody (clone 1H4) reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus) expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells; the expression of CD54 is upregulated by activation.
Protein A purified
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- Western Blot 1:1000
- Flow Cytometry 1 ug/ml
- Immunohistochemistry 1:10-1:500
- Immunohistochemistry-Frozen 1:200
- Immunohistochemistry-Paraffin 1:200
- Flow (Cell Surface)
Immunohistochemistry-Pparaffin sections recommended dilution: 10 ug/ml Application Note: Prolonged fixation in buffered formalin can destroy the epitope. Hight temperature antigen unmasking technique is required
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
PBS approx. pH 7.4; 0.2 um filter sterilized.
Protein A purified
Alternate Names for ICAM-1/CD54 Antibody (1H4)
- CD54 antigen
- cell surface glycoprotein P3.58
- human rhinovirus receptor
- intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
- intercellular adhesion molecule 1
- Major group rhinovirus receptor
CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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