ICAM-1/CD54 Antibody (1H4) [FITC] Summary
Raji cells and spleen cells fused with NS1 cells.
This antibody (clone 1H4) reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus) expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells; the expression of CD54 is upregulated by activation.
Protein A purified
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- Western Blot 1:100-1:2000
- Flow Cytometry
- Immunohistochemistry 1:10-1:500
- Immunohistochemistry-Frozen 1:10-1:500
- Immunohistochemistry-Paraffin 1:10-1:500
The reagent is designed for Flow Cytometry analysis of human blood cells using 20 ul reagent / 100 ul of whole blood or 10^6 cells in a suspension.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at 4C in the dark.
PBS and 0.2% (w/v) high grade BSA (protease free)
15mM Sodium Azide
Protein A purified
Alternate Names for ICAM-1/CD54 Antibody (1H4) [FITC]
- CD54 antigen
- cell surface glycoprotein P3.58
- human rhinovirus receptor
- intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
- intercellular adhesion molecule 1
- Major group rhinovirus receptor
CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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