ICAM-1/CD54 Antibody (1H4) [PE]

Flow Cytometry: ICAM-1/CD54 Antibody (1H4) [PE] [NB500-582] - Surface staining of human peripheral blood with anti-CD54 (1H4) PerCP.

Flow Cytometry: ICAM-1/CD54 Antibody (1H4) [PE] [NB500-582] - Surface staining of U937 human histiocytic lymphoma cell line with anti-human CD54 (1H4) PE. Total viable cells were used for analysis.

Flow Cytometry: ICAM-1/CD54 Antibody (1H4) [PE] [NB500-582] - Expression of CD54 in normal human peripheral blood, as detected with anti-CD54 (1H4) biotin.

• Reactivity: Hu
• Applications: Flow
• Clone: 1H4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: PE

ICAM-1/CD54 Antibody (1H4) [PE] Summary

• Immunogen: Raji cells and spleen cells fused with NS1 cells.
• Specificity: This antibody (clone 1H4) reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus) expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells; the expression of CD54 is upregulated by activation.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ICAM1
• Purity: Size Exclusion Chromatography

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
• Application Notes: The reagent is designed for Flow Cytometry analysis of human blood cells using 20 ul reagent / 100 ul of whole blood or 10^6 cells in a suspension.
• Theoretical MW: 110 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Purity: Size Exclusion Chromatography

Alternate Names for ICAM-1/CD54 Antibody (1H4) [PE]

• BB2
• CD54 antigen
• CD54
• cell surface glycoprotein P3.58
• human rhinovirus receptor
• ICAM1
• ICAM-1
• intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
• intercellular adhesion molecule 1
• Major group rhinovirus receptor
• P3.58

Background

CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ICAM1