ICAM-1/CD54 Antibody (1H4) [PE]

Images

 
Flow Cytometry: ICAM-1/CD54 Antibody (1H4) [PE] [NB500-582] - Flow Cytometry: ICAM1 Antibody (1H4) [PE] NB500-582]-Surface staining of U937 human histiocytic lymphoma cell line with anti-human CD54 (1H4) PE. Total ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
1H4
Clonality
Monoclonal
Host
Mouse
Conjugate
PE

ICAM-1/CD54 Antibody (1H4) [PE] Summary

Immunogen
Raji cells and spleen cells fused with NS1 cells.
Specificity
This antibody (clone 1H4) reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus) expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells; the expression of CD54 is upregulated by activation.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
ICAM1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
Application Notes
The reagent is designed for Flow Cytometry analysis of human blood cells using 20 ul reagent / 100 ul of whole blood or 10^6 cells in a suspension.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS and 0.2% (w/v) high grade BSA (protease free)
Preservative
15mM Sodium Azide
Purity
Protein A purified

Alternate Names for ICAM-1/CD54 Antibody (1H4) [PE]

  • BB2
  • CD54 antigen
  • CD54
  • cell surface glycoprotein P3.58
  • human rhinovirus receptor
  • ICAM1
  • ICAM-1
  • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
  • intercellular adhesion molecule 1
  • Major group rhinovirus receptor
  • P3.58

Background

CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NB500-584
FITC Labeled NB500-583

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Bioinformatics Tool for ICAM-1/CD54 Antibody (NB500-582)

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Bioinformatics

Gene Symbol ICAM1
Entrez