ICAM-1/CD54 Antibody (141011)

Western blot shows lysates of rat liver tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Rat ICAM-1/CD54 Monoclonal Antibody (Catalog # MAB583) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ICAM-1/CD54 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Rt
• Applications: WB
• Clone: 141011
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

ICAM-1/CD54 Antibody (141011) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant rat ICAM-1/CD54
  Met1-Thr493
  Accession # Q00238
• Specificity: Detects rat ICAM-1/CD54 in direct ELISAs and Western blots. Does not cross-react with recombinant mouse (rm) ICAM‑1, rmICAM-2, recombinant human (rh) ICAM-2, rhICAM-3, rmVCAM-1, or rhPCAM-1.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ICAM1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ICAM-1/CD54 Antibody (141011)

• BB2
• CD54 antigen
• CD54
• cell surface glycoprotein P3.58
• human rhinovirus receptor
• ICAM1
• ICAM-1
• intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
• intercellular adhesion molecule 1
• Major group rhinovirus receptor
• P3.58

Background

Intercellular Adhesion Molecule-1 (ICAM-1, CD54), binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma , TNF-alpha , IL-1 beta , and LPS. Rat and human ICAM-1 share approximately 52% amino acid identity. Rat and mouse ICAM-1 share approximately 52% amino acid identity.

Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer, and transplantation.

1. Pigott, R. and C. Power (1993) in The Adhesion Molecule Facts Book, p. 74. Academic Press.
2. Siu, G. et al. (1989) J. Immunol. 143:3813.
3. Ballantyne, C.M. et al. (1989) Nuc. Acid. Res. 17:5853.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ICAM-1/CD54 Antibody (MAB583)(3)

Publications using MAB583

• Xue Zhu, Ke Wang, Kai Zhang, Xuhua Tan, Zhifeng Wu, Song Sun et al. Tetramethylpyrazine Protects Retinal Capillary Endothelial Cells (TR-iBRB2) against IL-1 beta -Induced Nitrative/Oxidative Stress International Journal of Molecular Sciences 9/9/2015 [PMID: 26370989]
• Li PC, Chen WC, Chang LC, Lin SC Substance P acts via the neurokinin receptor 1 to elicit bronchoconstriction, oxidative stress, and upregulated ICAM-1 expression after oil smoke exposure. Am. J. Physiol. Lung Cell Mol. Physiol., 2008-03-07;294(5):L912-20. 2008-03-07 [PMID: 18326823] (Western Blot, Rat)
• McCarter SD, Mei SH, Lai PF, Zhang QW, Parker CH, Suen RS, Hood RD, Zhao YD, Deng Y, Han RN, Dumont DJ, Stewart DJ Cell-based angiopoietin-1 gene therapy for acute lung injury. Am. J. Respir. Crit. Care Med., 2007-02-22;175(10):1014-26. 2007-02-22 [PMID: 17322110] (Western Blot, Rat)

Bioinformatics

• Gene Symbol: ICAM1
• Uniprot:
  • Q00238()