HYPE Antibody (OTI2A11) - Azide and BSA Free

Western Blot: HYPE Antibody (OTI2A11) - Azide and BSA Free [NBP2-71681] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HYPE.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: OTI2A11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

HYPE Antibody (OTI2A11) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse HYPE Antibody (OTI2A11) - Azide and BSA Free (NBP2-45664) is a monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 61-265 of human FICD (NP_009007) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FICD
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500
• Theoretical MW: 51.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for HYPE Antibody (OTI2A11) - Azide and BSA Free

• AMPylator FICD
• EC 2.7.7.n1
• FIC domain containing
• FIC domain-containing protein
• fic S-phase protein cell division homolog
• HIP-13
• HIP13UNQ3041
• huntingtin interacting protein 13
• Huntingtin interacting protein E
• huntingtin interactor protein E
• Huntingtin yeast partner E
• Huntingtin-interacting protein 13
• Huntingtin-interacting protein E
• HYPEadenosine monophosphate-protein transferase FICD
• MGC5623

Background

Adenylyltransferase that mediates the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins. Able to inactivate Rho GTPases in vitro by adding AMP to RhoA, Rac and Cdc42. It is however unclear whether it inactivates GTPases in vivo and physiological substrates probably remain to be identified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FICD