Human TGF-beta 3 DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human TGF-beta 3 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: TGFB3

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human TGF-beta 3 DuoSet ELISA, 15 Plate

• ARVD
• ARVD1
• FLJ16571
• LDS5
• RNHF
• TGFB3
• TGFbeta 3
• TGF-beta 3
• TGF-beta3
• TGF-beta-3
• transforming growth factor beta-3
• transforming growth factor, beta 3

Background

Transforming Growth Factor Beta 1, 2, and 3 (TGF-beta 1, TGF-beta 2, and TGF-beta 3) are highly pleiotropic cytokines that virtually all cell types secrete. TGF-beta molecules are proposed to act as cellular switches that regulate processes such as immune function, proliferation, and epithelial-mesenchymal transition. Targeted deletions of these genes in mice show that each TGF-beta isoform has some non-redundant functions: TGF-beta 1 is involved in hematopoiesis and endothelial differentiation; TGF-beta 2 affects development of cardiac, lung, craniofacial, limb, eye, ear, and urogenital systems; and TGF-beta 3 influences palatogenesis and pulmonary development. The full range of in vitro biological activities of TGF-beta 5 has not yet been explored. However, TGF-beta 1, TGF-beta 2, TGF-beta 3, and TGF-beta 5 have been found to be largely interchangeable in an inhibitory bioassay, and it is anticipated that TGF-beta 5 will show a spectrum of activities similar to the other TGF-beta family members. To date, the production of TGF-beta 5 has only been demonstrated in Xenopus.

TGF-beta ligands are initially synthesized as precursor proteins that undergo proteolytic cleavage. The mature segments form active ligand dimers via a disulfide-rich core consisting of the characteristic 'cysteine knot'. TGF-beta signaling begins with binding to a complex of the accessory receptor betaglycan (also known as TGF-beta RIII) and a type II serine/threonine kinase receptor termed TGF-beta RII. This receptor then phosphorylates and activates a type I serine/threonine kinase receptor, either ALK-1 or TGF-beta RI (also called ALK-5). The activated type I receptor phosphorylates and activates Smad proteins that regulate transcription. Use of other signaling pathways that are Smad-independent allows for distinct actions observed in response to TGF-beta in different contexts.

Publications for TGF-beta 3 (DY243)(38)

Publications using DY243

• Li, H;Zhao, T;Yuan, Z;Gao, T;Yang, Y;Li, R;Tian, Q;Tang, P;Guo, Q;Zhang, L; Cartilage lacuna-biomimetic hydrogel microspheres endowed with integrated biological signal boost endogenous articular cartilage regeneration Bioactive materials 2024-11-01 [PMID: 39104774] (N/A)
• Bauer, B;Emonts, C;Pitts, J;Buhl, EM;Eschweiler, J;Hänsch, R;Betsch, M;Gries, T;Menzel, H; Topographically and Chemically Enhanced Textile Polycaprolactone Scaffolds for Tendon and Ligament Tissue Engineering Polymers 2024-02-09 [PMID: 38399866] (Human)
• Tietel, Z;Melamed, S;Ogen-Shtern, N;Eretz-Kdosha, N;Silberstein, E;Ayzenberg, T;Dag, A;Cohen, G; Topical application of jojoba (Simmondsia chinensis L.) wax enhances the synthesis of pro-collagen III and hyaluronic acid and reduces inflammation in the ex-vivo human skin organ culture model Frontiers in pharmacology 2024-01-26 [PMID: 38344180] (Human)
• Wang, T;Dogru, S;Dai, Z;Kim, SY;Vickers, NA;Albro, MB; Physiologic Doses of TGF-? Improve the Composition of Engineered Articular Cartilage bioRxiv : the preprint server for biology 2023-09-29 [PMID: 37808691] (Bovine)
• L Berten-Sch, Y Roger, H Bunjes, A Hoffmann Release of TGF-beta3 from Surface-Modified PCL Fiber Mats Triggers a Dose-Dependent Chondrogenic Differentiation of Human Mesenchymal Stromal Cells Pharmaceutics, 2023-04-21;15(4):. 2023-04-21 [PMID: 37111788] (Human)
• Y Zhao, Z Weng, X Zhou, Z Xu, B Cao, B Wang, J Li Mesenchymal stromal cells promote the drug resistance of gastrointestinal stromal tumors by activating the PI3K-AKT pathway via TGF-beta2 Journal of Translational Medicine, 2023-03-25;21(1):219. 2023-03-25 [PMID: 36966336] (Human)
• NM Martin, DE Griffin Effect of IL-10 Deficiency on TGFbeta Expression during Fatal Alphavirus Encephalomyelitis in C57Bl/6 Mice Viruses, 2022-08-16;14(8):. 2022-08-16 [PMID: 36016413] (Mouse)
• JP Lapuente, A Blázquez-M, J Marco-Brua, G Gómez, P Desportes, J Sanz, P Fernández, M García-Gil, F Bermejo, JV San Martín, A Algaba, JC De Gregori, D Lapuente, A De Gregori, B Lapuente, MV Andrés, A Anel Cytokine Profile and Anti-Inflammatory Activity of a Standardized Conditioned Medium Obtained by Coculture of Monocytes and Mesenchymal Stromal Cells (PRS CK STORM) Biomolecules, 2022-03-31;12(4):. 2022-03-31 [PMID: 35454123] (Human)
• D Halbgebaue, J Roos, JB Funcke, H Neubauer, BS Hamilton, E Simon, EZ Amri, KM Debatin, M Wabitsch, P Fischer-Po, D Tews Latent TGFbeta-binding proteins regulate UCP1 expression and function via TGFbeta2 Molecular Metabolism, 2021-09-01;53(0):101336. 2021-09-01 [PMID: 34481123] (Human)
• E Krepela, Z Vanickova, P Hrabal, M Zubal, B Chmielova, E Balaziova, P Vymola, I Matrasova, P Busek, A Sedo Regulation of Fibroblast Activation Protein by Transforming Growth Factor Beta-1 in Glioblastoma Microenvironment International Journal of Molecular Sciences, 2021-01-21;22(3):. 2021-01-21 [PMID: 33494271] (Human)
• LM Caballero, S Duchi, C Onofrillo, CD O'Connell, C Di Bella, SE Moulton Formation of alginate microspheres prepared by optimized microfluidics parameters for high encapsulation of bioactive molecules Journal of colloid and interface science, 2020-12-13;587(0):240-251. 2020-12-13 [PMID: 33360897] (Human)
• B Sun, M Lian, Y Han, X Mo, W Jiang, Z Qiao, K Dai A 3D-Bioprinted dual growth factor-releasing intervertebral disc scaffold induces nucleus pulposus and annulus fibrosus reconstruction Bioact Mater, 2020-08-14;6(1):179-190. 2020-08-14 [PMID: 32913927] (Rat)
• Z Mahmoudi, J Mohammadne, S Razavi Baz, A Abouei Meh, M Saidijam, R Dinarvand, M Ebrahimi W, M Soleimani Promoted chondrogenesis of hMCSs with controlled release of TGF-beta3 via microfluidics synthesized alginate nanogels Carbohydr Polym, 2019-11-07;229(0):115551. 2019-11-07 [PMID: 31826469] (Human)
• SP Roth, W Brehm, C Gro beta , P Scheibe, S Schubert, J Burk Transforming Growth Factor Beta 3-Loaded Decellularized Equine Tendon Matrix for Orthopedic Tissue Engineering Int J Mol Sci, 2019-11-03;20(21):. 2019-11-03 [PMID: 31684150] (Human)
• W He, L Chen, Y Huang, Z Xu, W Xu, N Ding, J Chen Synergistic effects of recombinant Lentiviral-mediated BMP2 and TGF-beta3 on the osteogenic differentiation of rat bone marrow mesenchymal stem cells in vitro Cytokine, 2019-04-13;120(0):1-8. 2019-04-13 [PMID: 30991228] (Rat)
• H Jing, B Gao, M Gao, H Yin, X Mo, X Zhang, K Luo, B Feng, W Fu, J Wang, W Zhang, M Yin, Z Zhu, X He, J Zheng Restoring tracheal defects in a rabbit model with tissue engineered patches based on TGF-?3-encapsulating electrospun poly(l-lactic acid-co-?-caprolactone)/collagen scaffolds Artif Cells Nanomed Biotechnol, 2018-02-15;0(0):1-11. 2018-02-15 [PMID: 29448837] (Human)
• J Denkovskij, E Bagdonas, I Kusleviciu, Z Mackiewicz, A Unguryte, N Porvanecka, S Fleury, A Venalis, C Jorgensen, E Bernotiene Paracrine Potential of the Human Adipose Tissue-Derived Stem Cells to Modulate Balance between Matrix Metalloproteinases and Their Inhibitors in the Osteoarthritic Cartilage In Vitro Stem Cells Int, 2017-07-27;2017(0):9542702. 2017-07-27 [PMID: 28819366] (Human)
• L Xie, M Mao, L Zhou, L Zhang, B Jiang Signal Factors Secreted by 2D and Spheroid Mesenchymal Stem Cells and by Cocultures of Mesenchymal Stem Cells Derived Microvesicles and Retinal Photoreceptor Neurons Stem Cells Int, 2017-01-18;2017(0):2730472. 2017-01-18 [PMID: 28194184] (Human)
• Meilang Xue The differential expression of protease activated receptors contributes to functional differences between dark and fair keratinocytes J. Dermatol. Sci, 2016-12-05;0(0):. 2016-12-05 [PMID: 27979585] (Human)
• Tao Cheng Neuronal Protein 3.1 Deficiency Leads to Reduced Cutaneous Scar Collagen Deposition and Tensile Strength due to Impaired Transforming Growth Factor-?1 to -?3 Translation Am. J. Pathol, 2016-12-08;0(0):. 2016-12-08 [PMID: 27939132] (Mouse)
• Young V, Brown J, Saunders P, Duncan W, Horne A The peritoneum is both a source and target of TGF-beta in women with endometriosis. PLoS ONE, 2014-09-10;9(9):e106773. 2014-09-10 [PMID: 25207642] (Human)
• Brunger J, Huynh N, Guenther C, Perez-Pinera P, Moutos F, Sanchez-Adams J, Gersbach C, Guilak F Scaffold-mediated lentiviral transduction for functional tissue engineering of cartilage. Proc Natl Acad Sci U S A, 2014-02-18;111(9):E798-806. 2014-02-18 [PMID: 24550481] (Human)
• Konkel J, Jin W, Abbatiello B, Grainger J, Chen W Thymocyte apoptosis drives the intrathymic generation of regulatory T cells. Proc Natl Acad Sci U S A, 2014-01-13;111(4):E465-73. 2014-01-13 [PMID: 24474796] (Mouse)
• Bialas A, Stevens B TGF-beta signaling regulates neuronal C1q expression and developmental synaptic refinement. Nat Neurosci, 2013-10-27;16(12):1773-82. 2013-10-27 [PMID: 24162655] (Rat)
• Lu C, Yeh T, Yeh C, Fang Y, Sung L, Lin S, Yen T, Chang Y, Hu Y Regenerating cartilages by engineered ASCs: prolonged TGF-beta3/BMP-6 expression improved articular cartilage formation and restored zonal structure. Mol Ther, 2013-07-15;22(1):186-95. 2013-07-15 [PMID: 23851345] (Rabbit)
• Hawinkels L, Paauwe M, Verspaget H, Wiercinska E, van der Zon J, van der Ploeg K, Koelink P, Lindeman J, Mesker W, ten Dijke P, Sier C Interaction with colon cancer cells hyperactivates TGF-beta signaling in cancer-associated fibroblasts. Oncogene, 2012-12-03;33(1):97-107. 2012-12-03 [PMID: 23208491] (Human)
• Pen A, Moreno MJ, Durocher Y, Deb-Rinker P, Stanimirovic DB Glioblastoma-secreted factors induce IGFBP7 and angiogenesis by modulating Smad-2-dependent TGF-beta signaling. Oncogene, 2008-08-18;27(54):6834-44. 2008-08-18 [PMID: 18711401] (Human)
• Gaide Chevronnay HP, Cornet PB, Delvaux D, Lemoine P, Courtoy PJ, Henriet P, Marbaix E Opposite regulation of transforming growth factors-beta2 and -beta3 expression in the human endometrium. Endocrinology, 2007-11-26;149(3):1015-25. 2007-11-26 [PMID: 18039789] (Human)
• Moioli EK, Clark PA, Sumner DR, Mao JJ Autologous stem cell regeneration in craniosynostosis. Bone, 2007-10-17;42(2):332-40. 2007-10-17 [PMID: 18023269] (Rat)
• Alcorn JF, Rinaldi LM, Jaffe EF, van Loon M, Bates JH, Janssen-Heininger YM, Irvin CG Transforming growth factor-beta1 suppresses airway hyperresponsiveness in allergic airway disease. Am. J. Respir. Crit. Care Med., 2007-08-29;176(10):974-82. 2007-08-29 [PMID: 17761617] (Mouse)
• Nagineni CN, Cherukuri KS, Kutty V, Detrick B, Hooks JJ Interferon-gamma differentially regulates TGF-beta1 and TGF-beta2 expression in human retinal pigment epithelial cells through JAK-STAT pathway. J. Cell. Physiol., 2007-01-01;210(1):192-200. 2007-01-01 [PMID: 17013806] (Human)
• Liu G, Ding W, Neiman J, Mulder KM Requirement of Smad3 and CREB-1 in mediating transforming growth factor-beta (TGF beta) induction of TGF beta 3 secretion. J. Biol. Chem., 2006-08-04;281(40):29479-90. 2006-08-04 [PMID: 16891311] (Rat)
• Ito M, Minamiya Y, Kawai H, Saito S, Saito H, Nakagawa T, Imai K, Hirokawa M, Ogawa J Tumor-derived TGFbeta-1 induces dendritic cell apoptosis in the sentinel lymph node. J. Immunol., 2006-05-01;176(9):5637-43. 2006-05-01 [PMID: 16622033] (Human)
• Neurohr C, Nishimura SL, Sheppard D Activation of transforming growth factor-beta by the integrin alphavbeta8 delays epithelial wound closure. Am. J. Respir. Cell Mol. Biol., 2006-03-30;35(2):252-9. 2006-03-30 [PMID: 16574941] (Human)
• Reinhold D, Perlov E, Schrecke K, Kekow J, Brune T, Sailer M Increased blood plasma concentrations of TGF-beta isoforms after treatment with intravenous immunoglobulins (i.v.IG) in patients with multiple sclerosis. J. Neuroimmunol., 2004-07-01;152(1):191-4. 2004-07-01 [PMID: 15223252] (Human)
• Rendi MH, Suh N, Lamph WW, Krajewski S, Reed JC, Heyman RA, Berchuck A, Liby K, Risingsong R, Royce DB, Williams CR, Sporn MB The selective estrogen receptor modulator arzoxifene and the rexinoid LG100268 cooperate to promote transforming growth factor beta-dependent apoptosis in breast cancer. Cancer Res., 2004-05-15;64(10):3566-71. 2004-05-15 [PMID: 15150113] (Rat)
• Jono H, Shuto T, Xu H, Kai H, Lim DJ, Gum JR, Kim YS, Yamaoka S, Feng XH, Li JD Transforming growth factor-beta -Smad signaling pathway cooperates with NF-kappa B to mediate nontypeable Haemophilus influenzae-induced MUC2 mucin transcription. J. Biol. Chem., 2002-09-16;277(47):45547-57. 2002-09-16 [PMID: 12237307] (Human)
• Luo XH, Liao EY, Su X Progesterone upregulates TGF-b isoforms (b1, b2, and b3) expression in normal human osteoblast-like cells. Calcif. Tissue Int., 2002-08-06;71(4):329-34. 2002-08-06 [PMID: 12154395] (Human)

Review for TGF-beta 3 (DY243) (1)

Average Rating: 5

(Based on 1 review)

reviewed by: Patricia Carulla Martí 06/22/2023

Summary

• Sample Tested: human dermal fibroblasts,Supernatant from Human primary fibroblasts (HDFa)
• Lot: P317296

FAQs for TGF-beta 3 (DY243). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: TGFB3