Human Pro-Collagen I alpha 1 DuoSet ELISA, 5 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human Pro-Collagen I alpha 1 DuoSet ELISA, 5 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: COL1A1

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Pro-Collagen I alpha 1 DuoSet ELISA, 5 Plate

• Alpha-1 type I collagen
• COL1A1
• COL-IA1
• Collagen 1 alpha 1
• Collagen 1
• collagen alpha 1 chain type I
• collagen alpha-1(I) chain
• Collagen I alpha 1
• collagen of skin, tendon and bone, alpha-1 chain
• Collagen Type I Alpha 1 Chain
• collagen, type I, alpha 1
• Collagen1
• Collagen-1
• EDSARTH1
• OI4
• pro-alpha-1 collagen type 1
• Type I Procollagen Alpha 1 Chain

Background

Type I collagen is the most abundant structural protein of connective tissues such as skin, bone and tendon. It is synthesized as a procollagen molecule which is characterized by a 300 nm triple helical domain flanked by globular N- and C-terminal propeptides. The triple helical domain contains Gly-Xaa-Yaa triplets where Xaa and Yaa are frequently proline and hydroxyproline, respectively. The non-helical propeptides are removed by procollagen N- and C-proteinase activities so that the mature triple helices can self-assemble into collagen fibrils that provide tensile strength to tissues. Type I collagen is a heterotrimer that consists of two alpha 1(I) chains and one alpha 2(I) chain, although homotrimers consisting of three identical alpha 1(I) chains have also been described. This recombinant mini pro-alpha 1(I) collagen consists of a shortened alpha 1(I) chain with following domain structure from N- to C-terminus: N-propeptide, N-telopeptide, the 33 most N-terminal Gly-Xaa-Yaa repeats, the 33 most C-terminal Gly-Xaa-Yaa repeats, C-telopeptide and C-propeptide. The preparation contains a mixture of the full-length molecule, pN collagen I (alpha 1) and the C-terminal propeptide. This truncated pro-alpha 1(I) collagen is a substrate for procollagen N-proteinase and procollagen C-proteinase.

Publications for Collagen I alpha 1 (DY6220-05)(61)

Publications using DY6220-05

• Mohammadalizadeh, Z;Estrada, KB;Almonte, M;Fogg, KC;Porras, AM; Developing a Cell Culture Protocol to Induce Quiescence in Intestinal Fibroblasts Using Design of Experiments Statistical Optimization Research square 2025-10-29 [PMID: 41282095] (Human)
• Kang, S;Park, J;Cho, E;Kim, D;Ye, S;Jeong, ET;Jun, SH;Kang, NG; Distinctive Gene Expression Profiles and Biological Responses of Skin Fibroblasts to Nicotinamide Mononucleotide: Implications for Longevity Effects on Skin Biomedicines 2025-09-29 [PMID: 41153679] (Human)
• Ye, JL;Grieger, K;Lu, D;Brandenberger, C;Juchem, M;Jordan, M;Oehlsen, L;Zardo, P;Werlein, C;Hesse, C;Sewald, K;Tretbar, S;Thum, T;Chatterjee, S;Bär, C; Telomerase modRNA Offers a Novel RNA-Based Approach to Treat Human Pulmonary Fibrosis Aging cell 2025-09-20 [PMID: 40974335] (Human)
• Min, S;Than, N;Shin, YC;Ertugral, EG;Kothapalli, CR;Awoniyi, O;Kim, HJ; Mechanostimulatory cues determine intestinal fibroblast fate and profibrotic remodeling in a physiodynamic human gut-on-a-chip bioRxiv : the preprint server for biology 2025-06-11 [PMID: 40661399] (Human)
• Xie, Y;van Daelen, M;Park, R;Almaraz, I;Fontenot, L;Rieder, F;Ho, W;Limketkai, B;Shih, DQ;Wei, M;Li, Y;Koon, HW; MicroRNA Sequencing of Serum Exosomes Reveals miR205-5p as an Anti-Fibrogenic Factor Against Intestinal Fibrosis in Crohn's Disease International journal of molecular sciences 2025-04-17 [PMID: 40332426] (Human)
• Bang, J;Hwang, YL;Kim, MY;Yun, JN;Hyun, E;Chang, MY;Shin, DH;Kim, S;Lee, JH; Wrinkle-Improving Effect of Novel Peptide That Binds to Nicotinic Acetylcholine Receptor International journal of molecular sciences 2024-07-18 [PMID: 39063099] (Human)
• Pinnarò, V;Kirchberger, S;Künig, S;Gil Cantero, S;Ciardulli, MC;Della Porta, G;Blüml, S;Elbe-Bürger, A;Bochkov, V;Stöckl, J; Oxidized Phospholipids Regulate Tenocyte Function via Induction of Amphiregulin in Dendritic Cells International journal of molecular sciences 2024-07-11 [PMID: 39062855] (Human)
• Aleman, J;Ravikumar, K;Wiegand, C;Schurdak, ME;Vernetti, L;Gavlock, D;Reese, C;DeBiasio, R;LaRocca, G;Angarita, YD;Gough, A;Soto-Gutierrez, A;Behari, J;Yechoor, V;Miedel, MT;Stern, AM;Banerjee, I;Taylor, DL; A metabolic-dysfunction associated steatotic liver acinus biomimetic induces pancreatic islet dysfunction in a coupled microphysiology system bioRxiv : the preprint server for biology 2024-04-30 [PMID: 38712135] (Human)
• Calver, J;Parmar, N;Harris, G;Lithgo, R;Stylianou, P;Zetterberg, F;Gooptu, B;MacKinnon, A;Carr, S;Borthwick, L;Scott, D;Stewart, I;Slack, R;Jenkins, G;John, A; Defining the mechanism of galectin-3-mediated TGF-?1 activation and its role in lung fibrosis Journal of Biological Chemistry 2024-04-18 [PMID: 38641066] (Human)
• Klatte-Schulz, F;Bormann, N;Bonell, A;Al-Michref, J;Nguyen, HL;Klöckner, P;Thiele, K;Moroder, P;Seifert, M;Sawitzki, B;Wildemann, B;Duda, GN; Pro-Resolving Mediators in Rotator Cuff Disease: How Is the Bursa Involved? Cells 2023-12-20 [PMID: 38201221] (Human)
• Lee, KS;Kim, Y;Lee, JH;Shon, S;Kim, A;Pham, AVQ;Kim, C;Kim, DH;Kim, YK;Cho, EG; Human Probiotic Lactobacillus paracasei-Derived Extracellular Vesicles Improve Tumor Necrosis Factor-?-Induced Inflammatory Phenotypes in Human Skin Cells 2023-12-07 [PMID: 38132109] (Human)
• Siquier-Dameto, G;Boisnic, S;Boadas-Vaello, P;Verdú, E; Anti-Aging and Depigmentation Effect of a Hyaluronic Acid Mechanically Stabilized Complex on Human Skin Explants Polymers 2023-05-24 [PMID: 37299236] (Human)
• SR Trampuž, S van Riet, Å Nordling, M Ingelman-S The Role of CTGF in Liver Fibrosis Induced in 3D Human Liver Spheroids Cells, 2023-01-13;12(2):. 2023-01-13 [PMID: 36672237] (Human)
• S Kang, H Lee, SH Jun, SG Park, NG Kang Enhancement of Efficacy of Retinoids through Enhancing Retinoid-Induced RAR Activity and Inhibiting Hydroxylation of Retinoic Acid, and Its Clinical Efficacy on Photo-Aging Pharmaceutics, 2022-11-08;14(11):. 2022-11-08 [PMID: 36365229] (Human)
• G Zhu, W Chen, CY Tang, A McVicar, D Edwards, J Wang, M McConnell, S Yang, Y Li, Z Chang, YP Li Knockout and Double Knockout of Cathepsin K and Mmp9 reveals a novel function of Cathepsin K as a regulator of osteoclast gene expression and bone homeostasis International journal of biological sciences, 2022-08-29;18(14):5522-5538. 2022-08-29 [PMID: 36147479] (Mouse)
• Kartasheva-Ebertz D, Gaston J, Lair-Mehiri L, Mottez E, Buivan T, Massault P, Scatton O, Gaujoux S, Vaillant J, Pol S, Lagaye S IL-17A in Human Liver: Significant Source of Inflammation and Trigger of Liver Fibrosis Initiation. Int J Mol Sci, 2022-08-29;23(17):. 2022-08-29 [PMID: 36077175] (Human)
• FA Fadeyev, FA Blyakhman, AP Safronov, GY Melnikov, AD Nikanorova, IP Novoselova, GV Kurlyandsk Biological Impact of gamma-Fe2O3 Magnetic Nanoparticles Obtained by Laser Target Evaporation: Focus on Magnetic Biosensor Applications Biosensors, 2022-08-11;12(8):. 2022-08-11 [PMID: 36005023] (Human)
• N Xia, Y Cai, W Wang, C Bao, Y Li, Q Xie, W Xu, D Liu Association of bone-related�biomarkers with femoral neck bone strength Bmc Musculoskeletal Disorders, 2022-05-21;23(1):482. 2022-05-21 [PMID: 35597928] (Human)
• MB da Cruz, JF Marques, BF Fernandes, S Madeira, Ó Carvalho, FS Silva, ADSP da Mata, JMM Caramês Peri-implant cell response on groove and pore-textured zirconia surfaces Journal of oral biosciences, 2022-02-07;0(0):. 2022-02-07 [PMID: 35143954] (Human)
• M Piñeiro-Ra, N Flórez-Fer, O Ramil-Góme, MD Torres, H Dominguez, FJ Blanco, R Meijide-Fa, C Vaamonde-G Antifibrotic effect of brown algae-derived fucoidans on osteoarthritic fibroblast-like synoviocytes Carbohydrate polymers, 2022-01-12;282(0):119134. 2022-01-12 [PMID: 35123730] (Human)
• A Chignon, D Argaud, MC Boulanger, G Mkannez, V Bon-Baret, Z Li, S Thériault, Y Bossé, P Mathieu Genome-wide chromatin contacts of super-enhancer-associated lncRNA identify LINC01013 as a regulator of fibrosis in the aortic valve PloS Genetics, 2022-01-18;18(1):e1010010. 2022-01-18 [PMID: 35041643] (Human)
• M Blanco, N Sanz, AC Sánzhez, B Correa, RI Pérez-Mart, CG Sotelo Molecular Weight Analysis of Blue Shark (Prionace glauca) Collagen Hydrolysates by GPC-LS; Effect of High Molecular Weight Hydrolysates on Fibroblast Cultures: mRNA Collagen Type I Expression and Synthesis International Journal of Molecular Sciences, 2021-12-21;23(1):. 2021-12-21 [PMID: 35008452] (Human)
• V Vorstandle, M Laggner, D Copic, K Klas, M Direder, Y Chen, B Golabi, W Haslik, C Radtke, E Tschachler, K Hötzenecke, HJ Ankersmit, M Mildner The serine proteases dipeptidyl-peptidase 4 and urokinase are key molecules in human and mouse scar formation Nature Communications, 2021-10-29;12(1):6242. 2021-10-29 [PMID: 34716325] (Human)
• E Dzia?o, M Czepiel, K Tkacz, M Siedlar, G Kania, P B?yszczuk WNT/beta-Catenin Signaling Promotes TGF-beta-Mediated Activation of Human Cardiac Fibroblasts by Enhancing IL-11 Production International Journal of Molecular Sciences, 2021-09-17;22(18):. 2021-09-17 [PMID: 34576234] (Human)
• K Liu, P Jing, Z Liu, Y Wang, Z Han, Y Wang, Z Zheng, Y Wu, T Wang, Y Li, H Zhang, L Wang Serum levels of irisin in postmenopausal women with osteoporotic hip fractures Cytokine, 2021-09-21;148(0):155708. 2021-09-21 [PMID: 34560610] (Human)
• CM Stanton, AS Findlay, C Drake, MZ Mustafa, P Gautier, L McKie, IJ Jackson, V Vitart A mouse model of brittle cornea syndrome caused by mutation in Zfp469 Disease Models & Mechanisms, 2021-09-22;14(9):. 2021-09-22 [PMID: 34368841] (Mouse, Transgenic Mouse)
• X Zhou, LJ Backman, P Danielson Activation of NF-&kapppaB signaling via cytosolic mitochondrial RNA sensing in kerotocytes with mitochondrial DNA common deletion Scientific Reports, 2021-04-01;11(1):7360. 2021-04-01 [PMID: 33795727] (Human)
• Y Xia, L Zhen, H Li, S Wang, S Chen, C Wang, X Yang MIRLET7BHG promotes hepatocellular carcinoma progression by activating hepatic stellate cells through exosomal SMO to trigger Hedgehog pathway Cell Death & Disease, 2021-03-26;12(4):326. 2021-03-26 [PMID: 33771969] (Human)
• P Zhou, G Chu, Z Yuan, H Wang, W Zhang, Y Mao, X Zhu, W Chen, H Yang, B Li Regulation of differentiation of annulus fibrosus-derived stem cells using heterogeneous electrospun fibrous scaffolds Journal of Orthopaedic Translation, 2020-03-03;26(0):171-180. 2020-03-03 [PMID: 33437636] (Rabbit)
• A Bayer, B Wijaya, L Möbus, F Rademacher, M Rodewald, M Tohidnezha, T Pufe, D Drücke, R Gläser, J Harder Platelet-Released Growth Factors and Platelet-Rich Fibrin Induce Expression of Factors Involved in Extracellular Matrix Organization in Human Keratinocytes 22.39 Int J Mol Sci, 2020-06-20;21(12):. 2020-06-20 [PMID: 32575800] (Human)
• JH Oh, YH Joo, F Karadeniz, J Ko, CS Kong Syringaresinol Inhibits UVA-Induced MMP-1 Expression by Suppression of MAPK/AP-1 Signaling in HaCaT Keratinocytes and Human Dermal Fibroblasts Int J Mol Sci, 2020-06-01;21(11):. 2020-06-01 [PMID: 32492931] (Human)
• B Baeza-Raja, A Goodyear, X Liu, K Lam, L Yamamoto, Y Li, GS Dodson, T Takeuchi, T Kisseleva, DA Brenner, K Dabbagh Pharmacological inhibition of P2RX7 ameliorates liver injury by reducing inflammation and fibrosis PLoS ONE, 2020-06-03;15(6):e0234038. 2020-06-03 [PMID: 32492075] (Human)
• P B?yszczuk, C Zuppinger, A Costa, D Nurzynska, FD Di Meglio, M Stellato, I Agarkova, GL Smith, O Distler, G Kania Activated Cardiac Fibroblasts Control Contraction of Human Fibrotic Cardiac Microtissues by a beta-Adrenoreceptor-Dependent Mechanism Cells, 2020-05-20;9(5):. 2020-05-20 [PMID: 32443848] (Human)
• E Bigaeva, N Puerta Cav, EGD Stribos, AJ de Jong, C Biel, HAM Mutsaers, MS Jensen, R Nørregaard, AM Leliveld, IJ de Jong, JL Hillebrand, H van Goor, M Boersema, RA Bank, P Olinga Predictive Value of Precision-Cut Kidney Slices as an Ex Vivo Screening Platform for Therapeutics in Human Renal Fibrosis Pharmaceutics, 2020-05-18;12(5):. 2020-05-18 [PMID: 32443499] (Human)
• JH Oh, F Karadeniz, JI Lee, SY Park, Y Seo, CS Kong Anticatabolic and Anti-Inflammatory Effects of Myricetin 3-O-&beta-d-Galactopyranoside in UVA-Irradiated Dermal Cells via Repression of MAPK/AP-1 and Activation of TGF&beta/Smad Molecules, 2020-03-14;25(6):. 2020-03-14 [PMID: 32183404] (Human)
• M Tozzi, CE Sørensen, L Magni, NM Christense, R Bouazzi, CM Buch, M Stefanini, C Duranti, A Arcangeli, I Novak Proton Pump Inhibitors Reduce Pancreatic Adenocarcinoma Progression by Selectively Targeting H+, K+-ATPases in Pancreatic Cancer and Stellate Cells Cancers (Basel), 2020-03-10;12(3):. 2020-03-10 [PMID: 32164284] (Mouse)
• HY Lim, D Jeong, SH Park, KK Shin, YH Hong, E Kim, YG Yu, TR Kim, H Kim, J Lee, JY Cho Antiwrinkle and Antimelanogenesis Effects of Tyndallized Lactobacillus acidophilus KCCM12625P Int J Mol Sci, 2020-02-27;21(5):. 2020-02-27 [PMID: 32120828] (Human)
• K Krempaska, S Barnowski, J Gavini, N Hobi, S Ebener, C Simillion, A Stokes, R Schliep, L Knudsen, TK Geiser, M Funke-Cham &alphaAzithromycin has enhanced effects on lung fibroblasts from idiopathic pulmonary fibrosis (IPF) patients compared to controls Respir. Res., 2020-01-15;21(1):25. 2020-01-15 [PMID: 31941499] (Human)
• J Chen, P Liang, Z Xiao, MF Chen, F Gong, C Li, C Zhou, P Hong, WK Jung, ZJ Qian Antiphotoaging effect of boiled abalone residual peptide ATPGDEG on UVB-induced keratinocyte HaCaT cells Food Nutr Res, 2019-11-08;63(0):. 2019-11-08 [PMID: 31762729] (Human)
• DB Myung, HS Han, JS Shin, JY Park, HJ Hwang, HJ Kim, HS Ahn, SH Lee, KT Lee Hydrangenol Isolated from the Leaves of Hydrangea serrata Attenuates Wrinkle Formation and Repairs Skin Moisture in UVB-Irradiated Hairless Mice Nutrients, 2019-10-02;11(10):. 2019-10-02 [PMID: 31581754] (Mouse)
• B Wu, XY Huang, L Li, XH Fan, PC Li, CQ Huang, J Xiao, R Gui, S Wang Attenuation of diabetic cardiomyopathy by relying on kirenol to suppress inflammation in a diabetic rat model J. Cell. Mol. Med., 2019-09-29;0(0):. 2019-09-29 [PMID: 31565849] (Rat)
• U Baranyi, B Winter, A Gugerell, B Hegedus, C Brostjan, G Laufer, B Messner Primary Human Fibroblasts in Culture Switch to a Myofibroblast-Like Phenotype Independently of TGF Beta Cells, 2019-07-13;8(7):. 2019-07-13 [PMID: 31337073] (Human)
• HX Zhang, YN Li, XL Wang, CL Ye, XY Zhu, HP Li, T Yang, YJ Liu Probucol ameliorates EMT and lung fibrosis through restoration of SIRT3 expression Pulm Pharmacol Ther, 2019-05-11;0(0):101803. 2019-05-11 [PMID: 31085231] (Mouse)
• E Dzia?o, M Rudnik, RI Koning, M Czepiel, K Tkacz, M Baj-Krzywo, O Distler, M Siedlar, G Kania, P B?yszczuk WNT3a and WNT5a Transported by Exosomes Activate WNT Signaling Pathways in Human Cardiac Fibroblasts Int J Mol Sci, 2019-03-21;20(6):. 2019-03-21 [PMID: 30901906] (Human)
• BD Sui, J Chen, XY Zhang, T He, P Zhao, CX Zheng, M Li, CH Hu, Y Jin Gender-independent efficacy of mesenchymal stem cell therapy in sex hormone-deficient bone loss via immunosuppression and resident stem cell recovery Exp. Mol. Med., 2018-12-17;50(12):166. 2018-12-17 [PMID: 30559383] (Human)
• RH Qin, H Tao, SH Ni, P Shi, C Dai, KH Shi microRNA-29a inhibits cardiac fibrosis in Sprague-Dawley rats by downregulating the expression of DNMT3A Anatol J Cardiol, 2018-10-01;20(4):198-205. 2018-10-01 [PMID: 30297596] (Rat)
• E Raschi, CB Chighizola, L Cesana, D Privitera, F Ingegnoli, C Mastaglio, PL Meroni, MO Borghi Immune complexes containing scleroderma-specific autoantibodies induce a profibrotic and proinflammatory phenotype in skin fibroblasts Arthritis Res. Ther., 2018-08-29;20(1):187. 2018-08-29 [PMID: 30157947] (Human)
• AR Han, TG Lim, YR Song, M Jang, YK Rhee, HD Hong, MH Kim, HJ Kim, CW Cho Inhibitory Effect of Opuntia humifusa Fruit Water Extract on Solar Ultraviolet-Induced MMP-1 Expression Int J Mol Sci, 2018-08-24;19(9):. 2018-08-24 [PMID: 30149526] (Human)
• Y Kondo, J Fu, H Wang, C Hoover, JM McDaniel, R Steet, D Patra, J Song, L Pollard, S Cathey, T Yago, G Wiley, S Macwana, J Guthridge, S McGee, S Li, C Griffin, K Furukawa, JA James, C Ruan, RP McEver, KJ Wierenga, PM Gaffney, L Xia Site-1 protease deficiency causes human skeletal dysplasia due to defective inter-organelle protein trafficking JCI Insight, 2018-07-26;3(14):. 2018-07-26 [PMID: 30046013] (Human)
• J Nanchahal, C Ball, D Davidson, L Williams, W Sones, FE McCann, M Cabrita, J Swettenham, NJ Cahoon, B Copsey, E Anne Franc, PC Taylor, J Black, VS Barber, S Dutton, M Feldmann, SE Lamb Anti-Tumour Necrosis Factor Therapy for Dupuytren's Disease: A Randomised Dose Response Proof of Concept Phase 2a Clinical Trial EBioMedicine, 2018-07-06;0(0):. 2018-07-06 [PMID: 29983350] (Human)
• Q Ma, Q Liu, L Yuan, Y Zhuang Protective Effects of LSGYGP from Fish Skin Gelatin Hydrolysates on UVB-Induced MEFs by Regulation of Oxidative Stress and Matrix Metalloproteinase Activity Nutrients, 2018-03-28;10(4):. 2018-03-28 [PMID: 29597313] (Mouse)
• A Aldhahrani, J Powell, S Ladak, M Ali, S Ali, B Verdon, J Pearson, C Ward The Potential Role of Bile Acids in Acquired Laryngotracheal Stenosis Laryngoscope, 2018-02-05;0(0):. 2018-02-05 [PMID: 29399801] (ELISA Capture, Human)
• A Beierfu beta , H Dietrich, C Kremser, M Hunjadi, A Ritsch, T Rülicke, C Thomé, DS Mern Knockout of Apolipoprotein E in rabbit promotes premature intervertebral disc degeneration: A new in vivo model for therapeutic approaches of spinal disc disorders PLoS ONE, 2017-11-03;12(11):e0187564. 2017-11-03 [PMID: 29099857] (Rabbit)
• E Martínez-M, J Ibarrola, A Fernández-, E Santamaria, J Fernández-, P Rossignol, F Jaisser, N López-Andr Differential Proteomics Identifies Reticulocalbin-3 as a Novel Negative Mediator of Collagen Production in Human Cardiac Fibroblasts Sci Rep, 2017-09-22;7(1):12192. 2017-09-22 [PMID: 28939891] (Human)
• M Rogers, N Maldonado-, JD Hutcheson, C Goettsch, S Goto, I Yamada, T Faits, H Sesaki, M Aikawa, E Aikawa Dynamin-Related Protein 1 Inhibition Attenuates Cardiovascular Calcification in the Presence of Oxidative Stress Circ. Res., 2017-06-12;0(0):. 2017-06-12 [PMID: 28607103] (Human)
• X Wu, D Bian, Y Dou, Z Gong, Q Tan, Y Xia, Y Dai Asiaticoside hinders the invasive growth of keloid fibroblasts through inhibition of the GDF-9/MAPK/Smad pathway J. Biochem. Mol. Toxicol, 2017-03-27;0(0):. 2017-03-27 [PMID: 28346732] (Human)
• Development of an in vitro human liver system for interrogating nonalcoholic steatohepatitis JCI Insight, 2016-12-08;1(20):e90954. 2016-12-08 [PMID: 27942596] (Human)
• Human pancreatic cancer cells induce a MyD88-dependent stromal response to promote a tumor-tolerant immune microenvironment Cancer Res., 2016-11-18;0(0):. 2016-11-18 [PMID: 27864347] (Human)
• S Robert, T Gicquel, A Bodin, V Lagente, E Boichot Characterization of the MMP/TIMP Imbalance and Collagen Production Induced by IL-1? or TNF-? Release from Human Hepatic Stellate Cells PLoS ONE, 2016-04-05;11(4):e0153118. 2016-04-05 [PMID: 27046197] (Human)
• Shan T, Zhang L, Zhao C, Chen W, Zhang Y, Li G Angiotensin-(1-7) and angiotensin induce the transdifferentiation of human endometrial epithelial cells in vitro. Mol Med Rep, 2014-04-09;9(6):2180-6. 2014-04-09 [PMID: 24718590] (Human)
• Martineau C, Martin-Falstrault L, Brissette L, Moreau R The atherogenic Scarb1 null mouse model shows a high bone mass phenotype. Am J Physiol Endocrinol Metab, 2013-11-19;306(1):E48-57. 2013-11-19 [PMID: 24253048] (Mouse)

Review for Collagen I alpha 1 (DY6220-05) (1)

Average Rating: 5

(Based on 1 review)

reviewed by: Patricia Carulla Martí 06/13/2023

Summary

• Sample Tested: Cell culture supernatant,HDFn culture supernatant
• Lot: p354148

FAQs for Collagen I alpha 1 (DY6220-05). (Showing 1 - 5 of 5 FAQs).

1. We are looking for an anti-Collagen antibody for Flow Cytometry (FACS) analysis in Swine sample. Would you please provide some references if there is any available as well?
   • We currently have one antibody for porcine Collagen validated in flow cytometry (catalog # NBP1-94202).
2. I am looking for anti collagen 1 antibody, I see your product calls it collagen I alpha 1 is there a difference?
   • Collagen I alpha 1 is the most common protein for the collagen 1 protein. There are other subunits as well (such as collagen 1 alpha 2…etc.).
3. The predicted molecular weight says 150kDA but the band on the gel appears at 130kDA why is that?
   • Differences between the predicted and observed MW can be affected by a number of factors including PTMs, relative charges and experimental factors. These cumulative effect of these differences can be quite substantial with larger molecules such as collagen I alpha 1.
4. How long does delivery take for your collagen 1 alpha 1 antibody?
   • Our most popular collagen I alpha 1 antibody (NB600-408) is usually in stock and ships priority overnight for next business day delivery in the U.S.
5. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: COL1A1