Human IL-23 Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human IL-23 Quantikine ELISA Kit Summary

• Background: The Quantikine Human IL-23 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human IL-23 in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human IL-23 and has been shown to accurately quantitate the recombinant factor. Results obtained for naturally occurring human IL-23 showed linear curves that were parallel to the standard curves... obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human IL-23.
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• Specificity: Natural and recombinant human IL-23. This assay does not recognize free p19 or p40 subunits.
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: IL23A

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-23 Quantikine ELISA Kit

• IL-23 p19/IL-12 p40
• IL23
• IL-23
• IL-23A
• IL-23-A
• IL-23p19
• IL-23p19/IL-12p40
• IL23P19P19
• interleukin 23 p19 subunit
• interleukin 23, alpha subunit p19
• interleukin-23 subunit alpha
• Interleukin-23 subunit p19
• JKA3 induced upon T-cell activation
• MGC79388
• SGRF
• SGRFIL-23 subunit alpha

Background

IL-23 (Interleukin-23) is a disulfide-linked cytokine composed of a p19 subunit that is unique to IL-23 and a p40 subunit that is shared with IL-12. It is produced by activated macrophages, microglia, and monocyte-derived dendritic cells in response to pathogens. IL-23 induces the earliest recruitment of neutrophils to the site of infection and promotes the development and maintenance of Th17 cells. It also enhances the development of Th17 mediated autoimmunity and tumor progression. IL-23 signals through a receptor complex consisting of IL-12 R beta 1 and IL-23 R. This complex is expressed in mouse Th1 and Th2 cells, bone marrow dendritic cells, activated macrophages and CD4+ CD45Rb(low) memory T cells.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-23A/IL-23 P19 (D2300B)(34)

Publications using D2300B

• Melgar-Rodríguez, S;Polanco, A;Ríos-Muñoz, J;García, M;Sierra-Cristancho, A;González-Osuna, L;Díaz-Zúñiga, J;Carvajal, P;Vernal, R;Bravo, D; Differential Response of Human Dendritic Cells upon Stimulation with Encapsulated or Non-Encapsulated Isogenic Strains of Porphyromonas gingivalis International journal of molecular sciences 2024-04-20 [PMID: 38674095] (Human)
• D Wang, Y Lin, F Xu, H Zhang, X Zhu, Z Liu, Y Hu, G Dong, B Sun, Y Yu, G Ma, Z Tang, D Legarda, A Ting, Y Liu, J Hou, L Dong, H Xiong SIRPalpha maintains macrophage homeostasis by interacting with PTK2B kinase in Mycobacterium tuberculosis infection and through autophagy and necroptosis EBioMedicine, 2022-10-03;85(0):104278. 2022-10-03 [PMID: 36202053] (Human)
• J Milosevic, M Jurisevic, V Grbovic, I Jovanovic, N Gajovic, A Jurisic-Sk Potential Protective Role of Galectin-3 in Patients with Gonarthrosis and Diabetes Mellitus: A Cross-Sectional Study International Journal of Environmental Research and Public Health, 2022-09-13;19(18):. 2022-09-13 [PMID: 36141752] (Human)
• N Millet, NV Solis, D Aguilar, MS Lionakis, RT Wheeler, N Jendzjowsk, M Swidergall IL-23 signaling prevents ferroptosis-driven renal immunopathology during candidiasis Nature Communications, 2022-09-22;13(1):5545. 2022-09-22 [PMID: 36138043] (Human)
• Q Su, L Zhang, Z Qi, B Huang The Mechanism of Inflammatory Factors and Soluble Vascular Cell Adhesion Molecule-1 Regulated by Nuclear Transcription Factor NF-kappaB in Unstable Angina Pectoris Journal of Immunology Research, 2022-07-30;2022(0):6137219. 2022-07-30 [PMID: 35942210] (Human)
• A Weber, A Schwiebs, H Solhaug, J Stenvik, AM Nilsen, M Wagner, B Relja, HH Radeke Nanoplastics affect the inflammatory cytokine release by primary human monocytes and dendritic cells Environment international, 2022-03-15;163(0):107173. 2022-03-15 [PMID: 35303527] (Human)
• HA Al-Rawaf, AH Alghadir, SA Gabr Expression of Circulating MicroRNAs and Myokines and Interactions with Serum Osteopontin in Type 2 Diabetic Patients with Moderate and Poor Glycemic Control: A Biochemical and Molecular Study BioMed Research International, 2021-12-07;2021(0):7453000. 2021-12-07 [PMID: 34917685] (Human)
• LA Lucaciu, M Ilie?, ?C Vesa, R Seicean, S Din, CA Iuga, A Seicean Serum Interleukin (IL)-23 and IL-17 Profile in Inflammatory Bowel Disease (IBD) Patients Could Differentiate between Severe and Non-Severe Disease Journal of personalized medicine, 2021-11-02;11(11):. 2021-11-02 [PMID: 34834482] (Human)
• VR Ribeiro, M Romao-Veig, PR Nunes, ML Matias, JC Peracoli, MTS Peracoli Vitamin D modulates the transcription factors of T cell subsets to anti-inflammatory and regulatory profiles in preeclampsia International immunopharmacology, 2021-11-20;0(0):108366. 2021-11-20 [PMID: 34810124] (Human)
• K Pawlak-Bu?, W Schmidt, P Leszczy?sk Lack of Association between Serum Interleukin-23 and Interleukin-27 Levels and Disease Activity in Patients with Active Systemic Lupus Erythematosus Journal of Clinical Medicine, 2021-10-19;10(20):. 2021-10-19 [PMID: 34682911] (Human)
• E Jahaj, AG Vassiliou, C Keskinidou, P Gallos, CS Vrettou, S Tsipilis, Z Mastora, SE Orfanos, I Dimopoulou, A Kotanidou Evaluating the Role of the Interleukin-23/17 Axis in Critically Ill COVID-19 Patients Journal of personalized medicine, 2021-09-07;11(9):. 2021-09-07 [PMID: 34575667] (Human)
• C Zhang, C Xu, L Gao, X Li, C Zhao Porphyromonas gingivalis lipopolysaccharide promotes T-hel per17 cell differentiation by upregulating Delta-like ligand 4 expression on CD14+ monocytes PeerJ, 2021-04-23;9(0):e11094. 2021-04-23 [PMID: 33981487] (Human)
• M Ga?ecka, K Bli?niewsk, A Orzechowsk, J Szemraj, M Maes, M Berk, KP Su, P Ga?ecki Inflammatory versus Anti-inflammatory Profiles in Major Depressive Disorders-The Role of IL-17, IL-21, IL-23, IL-35 and Foxp3 Journal of personalized medicine, 2021-01-23;11(2):. 2021-01-23 [PMID: 33498653] (Human)
• Gao Y, Sun W, Cha X, Wang H 'Psoriasis 1' reduces T‑lymphocyte‑mediated inflammation in patients with psoriasis by inhibiting vitamin D receptor‑mediated STAT4 inactivation. Int J Mol Med, 2020-08-06;46(4):1538-1550. 2020-08-06 [PMID: 32945358] (Human)
• MM Abhyankar, JZ Ma, KW Scully, AJ Nafziger, AL Frisbee, MM Saleh, GR Madden, AR Hays, M Poulter, WA Petri Immune Profiling To Predict Outcome of Clostridioides difficile Infection MBio, 2020-05-26;11(3):. 2020-05-26 [PMID: 32457246] (Human)
• H Yu, S Cui, Y Mei, Q Li, L Wu, S Duan, G Cai, H Zhu, B Fu, L Zhang, Z Feng, X Chen Mesangial Cells Exhibit Features of Antigen-Presenting Cells and Activate CD4+ T Cell Responses J Immunol Res, 2019-06-17;2019(0):2121849. 2019-06-17 [PMID: 31317046] (Human)
• JK Sun, WH Zhang, WX Chen, X Wang, XW Mu Effects of early enteral nutrition on Th17/Treg cells and IL-23/IL-17 in septic patients World J. Gastroenterol., 2019-06-14;25(22):2799-2808. 2019-06-14 [PMID: 31236002] (Human)
• S Clayton, E Cauble, A Kumar, N Patil, D Ledford, N Kolliputi, MF Lopes-Vire, D Castell, J Richter Plasma levels of TNF-?, IL-6, IFN-?, IL-12, IL-17, IL-22, and IL-23 in achalasia, eosinophilic esophagitis (EoE), and gastroesophageal reflux disease (GERD) BMC Gastroenterol, 2019-02-11;19(1):28. 2019-02-11 [PMID: 30744559] (Human)
• C Guida, SD Stephen, M Watson, N Dempster, P Larraufie, T Marjot, T Cargill, L Rickers, M Pavlides, J Tomlinson, JFL Cobbold, CM Zhao, D Chen, F Gribble, F Reimann, R Gillies, B Sgromo, P Rorsman, JD Ryan, RD Ramracheya PYY plays a key role in the resolution of diabetes following bariatric surgery in humans EBioMedicine, 2019-01-11;0(0):. 2019-01-11 [PMID: 30639417] (Human)
• N Ma, Q Tang, WT Wu, XA Huang, Q Xu, GL Rong, S Chen, JP Song Three Constituents of Moringa oleifera Seeds Regulate Expression of Th17-Relevant Cytokines and Ameliorate TPA-Induced Psoriasis-Like Skin Lesions in Mice Molecules, 2018-12-10;23(12):. 2018-12-10 [PMID: 30544700] (Human)
• T Zheng, C Xu, C Mao, X Mou, F Wu, X Wang, L Bu, Y Zhou, X Luo, Q Lu, H Liu, G Yuan, S Wang, D Chen, Y Xiao Increased Interleukin-23 in Hashimoto's Thyroiditis Disease Induces Autophagy Suppression and Reactive Oxygen Species Accumulation Front Immunol, 2018-01-29;9(0):96. 2018-01-29 [PMID: 29434604] (Human)
• L Srinivasan, L Kilpatrick, SS Shah, S Abbasi, MC Harris Elevations of novel cytokines in bacterial meningitis in infants PLoS ONE, 2018-02-02;13(2):e0181449. 2018-02-02 [PMID: 29394248] (Human)
• G Schmalz, I Davarpanah, J Jäger, RF Mausberg, B Krohn-Grim, J Schmidt, R Haak, U Sack, D Ziebolz MMP-8 and TIMP-1 are associated to periodontal inflammation in patients with rheumatoid arthritis under methotrexate immunosuppression - First results of a cross-sectional study J Microbiol Immunol Infect, 2017-09-04;0(0):. 2017-09-04 [PMID: 28919284] (Human)
• Z Meng, Y Zhang, Z Wei, P Liu, J Kang, Y Zhang, D Ma, C Ke, Y Chen, J Luo, Z Gong High serum resistin associates with intrahepatic inflammation and necrosis: an index of disease severity for patients with chronic HBV infection BMC Gastroenterol, 2017-01-07;17(1):6. 2017-01-07 [PMID: 28061755] (Human)
• Jennifer L Liedel Effects of anticipated neonatal surgical intervention on maternal milk cytokine production J. Pediatr. Surg., 2016-10-27;0(0):. 2016-10-27 [PMID: 27836369] (Human)
• Ofer Levy Age-Specific Adjuvant Synergy: Dual TLR7/8 and Mincle Activation of Human Newborn Dendritic Cells Enables Th1 Polarization J. Immunol., 2016-10-28;0(0):. 2016-10-28 [PMID: 27793997] (Human)
• Serum Interleukin-18, Fetuin-A, Soluble Intercellular Adhesion Molecule-1, and Endothelin-1 in Ankylosing Spondylitis, Psoriatic Arthritis, and SAPHO Syndrome Int J Mol Sci, 2016-08-03;17(8):. 2016-08-03 [PMID: 27527149] (Human)
• Loubaki L, Hadj-Salem I, Fakhfakh R, Jacques E, Plante S, Boisvert M, Aoudjit F, Chakir J Co-culture of human bronchial fibroblasts and CD4+ T cells increases Th17 cytokine signature. PLoS ONE, 2013-12-05;8(12):e81983. 2013-12-05 [PMID: 24349168] (Human)
• Grivennikov S, Wang K, Mucida D, Stewart C, Schnabl B, Jauch D, Taniguchi K, Yu G, Osterreicher C, Hung K, Datz C, Feng Y, Fearon E, Oukka M, Tessarollo L, Coppola V, Yarovinsky F, Cheroutre H, Eckmann L, Trinchieri G, Karin M Adenoma-linked barrier defects and microbial products drive IL-23/IL-17-mediated tumour growth. Nature, 2012-11-08;491(7423):254-8. 2012-11-08 [PMID: 23034650] (Human)
• Nakajima H, Nakajima K, Tarutani M, Morishige R, Sano S Kinetics of circulating Th17 cytokines and adipokines in psoriasis patients. Arch. Dermatol. Res., 2011-06-17;303(6):451-5. 2011-06-17 [PMID: 21681565] (Human)
• Song W, Tai YT, Tian Z, Hideshima T, Chauhan D, Nanjappa P, Exley MA, Anderson KC, Munshi NC HDAC inhibition by LBH589 affects the phenotype and function of human myeloid dendritic cells. Leukemia, 2010-11-19;25(1):161-8. 2010-11-19 [PMID: 21102427] (Human)
• Legat A, Thomas S, Hermand P, Van Mechelen M, Goldman M, De Wit D CD14-independent responses induced by a synthetic lipid A mimetic. Eur J Immunol, 2010-03-01;40(3):797-802. 2010-03-01 [PMID: 20039305] (Human)
• Eid RE, Rao DA, Zhou J, Lo SF, Ranjbaran H, Gallo A, Sokol SI, Pfau S, Pober JS, Tellides G Interleukin-17 and interferon-gamma are produced concomitantly by human coronary artery-infiltrating T cells and act synergistically on vascular smooth muscle cells. Circulation, 2009-03-02;119(10):1424-32. 2009-03-02 [PMID: 19255340] (Human)
• Chi W, Zhu X, Yang P, Liu X, Lin X, Zhou H, Huang X, Kijlstra A Upregulated IL-23 and IL-17 in Behcet patients with active uveitis. Invest. Ophthalmol. Vis. Sci., 2008-07-01;49(7):3058-64. 2008-07-01 [PMID: 18579762] (Human)

FAQs for IL-23A/IL-23 P19 (D2300B). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IL23A