Human IL-17 DuoSet ELISA, 15 Plate

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
Biotin

Order Details

Human IL-17 DuoSet ELISA, 15 Plate Summary

Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
IL17A

Applications/Dilutions

Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using
DY317 in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-17 DuoSet ELISA, 15 Plate

  • CTLA8
  • CTLA-8
  • CTLA8cytotoxic T-lymphocyte-associated serine esterase 8
  • Cytotoxic T-lymphocyte-associated antigen 8
  • IL17
  • IL-17
  • IL17A
  • IL-17A
  • IL-17Acytotoxic T-lymphocyte-associated protein 8
  • IL-17CTLA-8
  • IL17interleukin-17A
  • interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
  • interleukin 17A

Background

Human Interleukin 17 (IL-17), also known as IL-17A and CTLA-8, is a 15-20 kDa, variably glycosylated polypeptide that belongs to the IL-17 family of cytokines (1-5). Its alternate name, CTLA-8, originated from rodent studies where an activated hybridoma was created from the fusion of a mouse cytotoxic and a rat T cell lymphoma cell line. The molecule of interest in this study was assumed to have come from the mouse cytotoxic lymphocyte cell (thus the CTL designation), whereas, in fact, it was a rat lymphocyte molecule. Human IL-17/17A is synthesized as a 155 amino acid (aa) precursor that contains a 23 aa signal sequence and a 133 aa mature region that possesses a cysteine-knot fold (4-6). In both human and mouse, there is one conserved N-linked glycosylation site that likely contributes 5 kDa to its native molecular weight. IL-17A forms both a 32-38 kDa disulfide-linked homodimer, and a 40-45 kDa covalent heterodimer with IL-17F (7-9). Most secreted IL-17A is in the form of the IL-17A:F heterodimer, however, the IL-17A:A homodimer is the most bioactive of the two forms (8). Mature human IL-17A is 61%, 74%, and 99% aa identical to mouse, porcine, and chimpanzee IL-17A, respectively (10-12). Mammalian cells known to produce IL-17 are the CD4+ Th17 T cells, Paneth cells, GR1+CD11b+ myeloid suppressor cells, CD27- gamma δ T cells, CD1+NK1.1- iNKT cells, and CD3- CD4+ LTi-like cells (9, 13-17). 
A high affinity receptor for human IL-17 has been reported, and appears to be a heteromultimer of IL-17RA and IL-17RC, likely in a 2:1 ratio (1, 18). IL-17RA is a 130 kDa, type I transmembrane glycoprotein that bears no resemblance to members of the cytokine, TNF or immunoglobulin receptor superfamily (2, 10, 15). IL-17RC is also a type I transmembrane protein, approximately 90-95 kDa in size, that shares less than 30% aa identity with IL-17RA (19, 20). Both receptors are needed for IL-17A and IL-17A:F activity. The two receptors appear to form a functional association following ligand binding to IL-17RA (1, 21, 22). 
IL-17 is best known for its participation in the recruitment and survival of neutrophils (14, 15, 23, 24, 25). Its induction was initially described to be the result of antigen stimulation of dendritic cells, resulting in IL-23 secretion. In a T cell receptor-independent event, IL-23 induces T cell production of IL-17 (14). Once secreted, IL-17 in the bone marrow would seem to induce stromal/fibroblast expression of both G-CSF and stem cell factor (membrane form), an effect that increases polymorphonuclear neutrophils (PMN) differentiation and production. IL-17 may complement this by directly blocking neutrophil apoptosis, promoting greater circulating PMN numbers (23). In the tissues, IL-17 would also seem to promote neutrophil extravasation, principally through its effects on macrophages and endothelial cells (EC). On macrophages, IL-17 induces TNF-alpha, IL-1b and IL-6 production (26). TNF-alpha and IL-1b then act on local ECs to induce G-CSF secretion, an effect that is potentiated by IL-17 (27). IL-17 further contributes to PMN influx by inducing EC CXC chemokine release and NO production, which may increase vascular permeability (14, 28). IL-17 effects are not limited to inflammation. In synovial joints, IL-17 upregulates RANKL expression on osteoblasts. This provides a stimulus for osteoclast formation and subsequent bone resorption (24). In conjunction with IL-4 and CD40L, IL-17A also promotes the generation of IgE secreting cells (29). And in white fat, IL-17A inhibits adipocyte differentiation from preadipocytes, and impairs glucose uptake by mature adipocytes (30).

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Publications for IL-17/IL-17A (DY317)(46)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Neutralization.


Filter By Application
Neutralization
(1)
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Human
(46)
All Species
Showing Publications 1 - 10 of 46. Show All 46 Publications.
Publications using DY317 Applications Species
T Maekawa, H Tamura, H Domon, T Hiyoshi, T Isono, D Yonezawa, N Hayashi, N Takahashi, K Tabeta, T Maeda, M Oda, A Ziogas, V? Alexaki, T Chavakis, Y Terao, G Hajishenga Erythromycin inhibits neutrophilic inflammation and mucosal disease by upregulating DEL-1 JCI Insight, 2020;0(0):. 2020 [PMID: 32603314] (Human) Human
M Herrera, C Vera, Y Keynan, ZV Rueda Gaps in Study Design for Immune Parameter Research for Latent Tuberculosis Infection: A Systematic Review J Immunol Res, 2020;2020(0):8074183. 2020 [PMID: 32377537] (Human) Human
PX Losada, F Perdomo-Ce, M Castro, C Salcedo, A Salcedo, I DeLaura, G Lastra, CF Narváez Locally-secreted interleukin-6 is related with radiological severity in smear-negative pulmonary tuberculosis Cytokine, 2019;127(0):154950. 2019 [PMID: 31864093] (Human) Human
J Qi, L Du, J Deng, Y Qin, G Su, S Hou, M Lv, Q Zhang, A Kijlstra, P Yang Replication of Genome-Wide Association Analysis Identifies New Susceptibility Loci at Long Noncoding RNA Regions for Vogt-Koyanagi-Harada Disease Invest. Ophthalmol. Vis. Sci., 2019;60(14):4820-4829. 2019 [PMID: 31747682] (Human) Human
S Martínez-H, V Sánchez-Ga, A Herrero-Ce, Á Vinué, JT Real, JF Ascaso, DJ Burks, H González-N Type 1 diabetic mellitus patients with increased atherosclerosis risk display decreased CDKN2A/2B/2BAS gene expression in leukocytes J Transl Med, 2019;17(1):222. 2019 [PMID: 31299986] (Human) Human
T Lopatina, E Favaro, C Grange, M Cedrino, A Ranghino, S Occhipinti, S Fallo, F Buffolo, DA Gaykalova, MM Zanone, R Romagnoli, G Camussi PDGF enhances the protective effect of adipose stem cell-derived extracellular vesicles in a model of acute hindlimb ischemia Sci Rep, 2018;8(1):17458. 2018 [PMID: 30514962] (Human) Human
S Yi, R Chang, J Hu, Y Qiu, Q Wang, Q Cao, G Yuan, G Su, C Zhou, Y Wang, A Kijlstra, P Yang Disabled-2 (DAB2) Overexpression Inhibits Monocyte-Derived Dendritic Cells' Function in Vogt-Koyanagi-Harada Disease Invest. Ophthalmol. Vis. Sci., 2018;59(11):4662-4669. 2018 [PMID: 30267088] (Human) Human
Q Zhang, H Li, S Hou, H Yu, G Su, B Deng, J Qi, C Zhou, A Kijlstra, P Yang Association of genetic variations in PTPN2 and CD122 with ocular Behcet's disease Br J Ophthalmol, 2018;0(0):. 2018 [PMID: 29502070] (Human) Human
HC Tsa, S Velichko, S Lee, R Wu Cholera toxin enhances IL-17A production in both CD4and CD8cells via a cAMP/PKA-mediated IL-17A promoter activation Immunology, 2018;0(0):. 2018 [PMID: 29377102] (Human) Human
M Manetti, S Pratesi, E Romano, S Bellando-R, I Rosa, S Guiducci, BS Fioretto, L Ibba-Manne, E Maggi, M Matucci-Ce Angiogenic T cell expansion correlates with severity of peripheral vascular damage in systemic sclerosis PLoS ONE, 2017;12(8):e0183102. 2017 [PMID: 28797111] (Human) Human
Show All 46 Publications.

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Product General Protocols

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FAQs for IL-17/IL-17A (DY317). (Showing 1 - 3 of 3 FAQs).

  1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
    • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
  2. I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.
    • Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the following link to see the available fluors: http://www.novusbio.com/support/custom-antibody-labeling.html The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).
  3. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Other Available Formats

Biotin DY317
Biotin DY317

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Bioinformatics

Gene Symbol IL17A