Human ICAM-1/CD54 DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human ICAM-1/CD54 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: ICAM1

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human ICAM-1/CD54 DuoSet ELISA, 15 Plate

• BB2
• CD54 antigen
• CD54
• cell surface glycoprotein P3.58
• human rhinovirus receptor
• ICAM1
• ICAM-1
• intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
• intercellular adhesion molecule 1
• Major group rhinovirus receptor
• P3.58

Background

ICAM-1 (intercellular adhesion molecule-1), also known as CD54, is a transmembrane protein that is upregulated on endothelial and epithelial cells at sites of inflammation. It mediates the vascular adhesion and paracellular migration of leukocytes expressing activated LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). It also binds several non-integrin ligands including CD43/Sialophorin, Fibrinogen, Hyaluronan, rhinoviruses, and Plasmodium falciparum-infected erythrocytes. Soluble ICAM-1 promotes angiogenesis and serves an indicator of vascular endothelial cell activation or damage. Elevated levels of soluble ICAM-1 are associated with cardiovascular disease, type 2 diabetes, organ transplant dysfunction, oxidant stress, increased abdominal fat mass, hypertension, liver disease, certain malignancies, and cerebral malaria.

Publications for ICAM-1/CD54 (DY720)(50)

Publications using DY720

• Rezaei, A;Pourfarzam, S;Jamali, T;Mohammadi, NG;Andalib, A;Hassan, ZM;Ayubi, F;Ghazanfari, T; Exploring chemokines and soluble adhesion molecules in mustard lung pathogenesis International immunopharmacology 2025-02-08 [PMID: 39923581] (Human)
• Han, H;Lee, S;Gao, G;Yi, HG;Paek, SH;Jang, J; Cerebrovascular-Specific Extracellular Matrix Bioink Promotes Blood-Brain Barrier Properties Biomaterials research 2024-12-05 [PMID: 39641002] (Human)
• Hayderi, A;Zegeye, MM;Meydan, S;Sirsjö, A;Kumawat, AK;Ljungberg, LU; TNF Induces Laminin-332-Encoding Genes in Endothelial Cells and Laminin-332 Promotes an Atherogenic Endothelial Phenotype International journal of molecular sciences 2024-08-09 [PMID: 39201392] (Human)
• Ekelund, C;Dereke, J;Nilsson, C;Landin-Olsson, M; Are soluble E-selectin, ICAM-1, and VCAM-1 potential predictors for the development of diabetic retinopathy in young adults, 15-34 years of age? A Swedish prospective cohort study PloS one 2024-06-06 [PMID: 38843125] (Human)
• Rodríguez-Hernández, MÁ;Baena-Bustos, M;Carneros, D;Zurita-Palomo, C;Muñoz-Pinillos, P;Millán, J;Padillo, FJ;Smerdou, C;von Kobbe, C;Rose-John, S;Bustos, M; Targeting IL-6 trans-signalling by sgp130Fc attenuates severity in SARS-CoV-2 -infected mice and reduces endotheliopathy EBioMedicine 2024-04-26 [PMID: 38677182] (Transgenic Mouse)
• Alehagen, U;Alexander, J;Aaseth, JO;Larsson, A;Svensson, E;Opstad, TB; Effects of an Intervention with Selenium and Coenzyme Q10 on Five Selected Age-Related Biomarkers in Elderly Swedes Low in Selenium: Results That Point to an Anti-Ageing Effect-A Sub-Analysis of a Previous Prospective Double-Blind Placebo-Controlled Randomised Clinical Trial Cells 2023-07-04 [PMID: 37443807] (Human)
• Kirsten, N;Ohmes, J;Mikkelsen, MD;Nguyen, TT;Blümel, M;Wang, F;Tasdemir, D;Seekamp, A;Meyer, AS;Fuchs, S; Impact of Enzymatically Extracted High Molecular Weight Fucoidan on Lipopolysaccharide-Induced Endothelial Activation and Leukocyte Adhesion Marine drugs 2023-05-31 [PMID: 37367664] (Human)
• VC Capetini, BJ Quintanilh, DC de Oliveir, AH Nishioka, LA de Matos, LRP Ferreira, FM Ferreira, GR Sampaio, NMA Hassimotto, FM Lajolo, RA Fock, MM Rogero Blood orange juice intake modulates plasma and PBMC microRNA expression in overweight and insulin resistance women: impact on MAPK and NFkappaB signaling pathways The Journal of nutritional biochemistry, 2022-11-26;0(0):109240. 2022-11-26 [PMID: 36442716] (Human)
• D Azzopardi, LE Haswell, J Frosina, M McEwan, N Gale, J Thissen, F Meichanetz, G Hardie Biomarkers of Exposure and Potential Harm in Exclusive Users of Nicotine Pouches and Current, Former, and Never Smokers: Protocol for a Cross-sectional Clinical Study JMIR research protocols, 2022-10-06;11(10):e39785. 2022-10-06 [PMID: 36201395] (Human)
• ML Sarangam, R Namazzi, D Datta, C Bond, CPB Vanderpool, RO Opoka, CC John, AL Conroy Intestinal Injury Biomarkers Predict Mortality in Pediatric Severe Malaria MBio, 2022-09-07;0(0):e0132522. 2022-09-07 [PMID: 36069443] (Human)
• S Rutecki, P Szulc, M Paku?a, P Uruski, A Radziemski, E Naumowicz, R Moszy?ski, A Tykarski, J Miku?a-Pie, K Ksi??ek Pro-cancerogenic effects of spontaneous and drug-induced senescence of ovarian cancer cells in vitro and in vivo: a comparative analysis Journal of ovarian research, 2022-07-26;15(1):87. 2022-07-26 [PMID: 35883110] (Human)
• J Ohmes, MD Mikkelsen, TT Nguyen, VHN Tran, S Meier, MS Nielsen, M Ding, A Seekamp, AS Meyer, S Fuchs Depolymerization of fucoidan with endo-fucoidanase changes bioactivity in processes relevant for bone regeneration Carbohydrate polymers, 2022-02-24;286(0):119286. 2022-02-24 [PMID: 35337530] (Human)
• D Sikorska, D Kami?ska, R Catar, M Banasik, H Heidecke, K Schulze-Fo, K Korybalska, R Rutkowski, J ?uczak, J Jab?ecki, A Oko, P Daroszewsk, M Kusztal, W Samborski Non-HLA Antibodies in Hand Transplant Recipients Are Connected to Multiple Acute Rejection Episodes and Endothelial Activation Journal of Clinical Medicine, 2022-02-04;11(3):. 2022-02-04 [PMID: 35160284] (Human)
• J Villar, R Herrán-Mon, E González-H, M Prieto-Gon, A Ambrós, A Rodríguez-, A Muriel-Bom, R Solano, C Cuenca-Rub, A Vidal, C Flores, JM González-M, MI García-Lao, Genetics o Clinical and biological markers for predicting ARDS and outcome in septic patients Scientific Reports, 2021-11-22;11(1):22702. 2021-11-22 [PMID: 34811434] (Human)
• HW Lee, V Nizet, JN An, HS Lee, YR Song, SG Kim, JK Kim Uremic serum damages endothelium by provoking excessive neutrophil extracellular trap formation Scientific Reports, 2021-11-02;11(1):21439. 2021-11-02 [PMID: 34728714] (Human)
• E Wirthgen, M Hornschuh, IM Wrobel, C Manteuffel, J Däbritz Mimicking of Blood Flow Results in a Distinct Functional Phenotype in Human Non-Adherent Classical Monocytes Biology, 2021-08-04;10(8):. 2021-08-04 [PMID: 34439980] (Human)
• J Budzianows, J Hiczkiewic, K ?ojewska, E Kawka, R Rutkowski, K Korybalska Predictors of Early-Recurrence Atrial Fibrillation after Catheter Ablation in Women and Men with Abnormal Body Weight Journal of Clinical Medicine, 2021-06-18;10(12):. 2021-06-18 [PMID: 34207297] (Human)
• RJ Playford, N Choudhry, P Kelly, T Marchbank Effects of Bovine Colostrum with or without Egg on In Vitro Bacterial-Induced Intestinal Damage with Relevance for SIBO and Infectious Diarrhea Nutrients, 2021-03-22;13(3):. 2021-03-22 [PMID: 33809940] (Human)
• AE Wyman, TTT Nguyen, P Karki, ME Tulapurkar, CO Zhang, J Kim, TG Feng, AJ Dabo, NW Todd, IG Luzina, P Geraghty, RF Foronjy, JD Hasday, AA Birukova, SP Atamas, KG Birukov SIRT7 deficiency suppresses inflammation, induces EndoMT, and increases vascular permeability in primary pulmonary endothelial cells Sci Rep, 2020-07-27;10(1):12497. 2020-07-27 [PMID: 32719338] (Human)
• EB Volokhina, WJC Feitz, LM Elders, TJAM van der Ve, NCAJ van de Kar, LPWJ van den He Shiga Toxin Selectively Upregulates Expression of Syndecan-4 and Adhesion Molecule ICAM-1 in Human Glomerular Microvascular Endothelium Toxins (Basel), 2020-07-03;12(7):. 2020-07-03 [PMID: 32635212] (Human)
• CM Weight, C Venturini, S Pojar, SP Jochems, J Reiné, E Nikolaou, C Solórzano, M Noursadegh, JS Brown, DM Ferreira, RS Heyderman Microinvasion by Streptococcus pneumoniae induces epithelial innate immunity during colonisation at the human mucosal surface Nat Commun, 2019-07-16;10(1):3060. 2019-07-16 [PMID: 31311921] (Human)
• M Paku?a, P Uruski, A Niklas, A Wo?niak, D Szpurek, A Tykarski, J Miku?a-Pie, K Ksi??ek A Unique Pattern of Mesothelial-Mesenchymal Transition Induced in the Normal Peritoneal Mesothelium by High-Grade Serous Ovarian Cancer Cancers (Basel), 2019-05-13;11(5):. 2019-05-13 [PMID: 31086083] (Human)
• CR McDonald, AM Weckman, AL Conroy, P Olwoch, P Natureeba, MR Kamya, DV Havlir, G Dorsey, KC Kain Systemic inflammation is associated with malaria and preterm birth in women living with HIV on antiretrovirals and co-trimoxazole Sci Rep, 2019-05-01;9(1):6758. 2019-05-01 [PMID: 31043691] (Human)
• I Govorov, K Bremme, A Larsson, M Holmström, E Komlichenk, R Chaireti, M Mints Blood inflammatory and endothelial markers in women with von Willebrand disease PLoS ONE, 2019-01-10;14(1):e0210544. 2019-01-10 [PMID: 30629692] (Human)
• MC Chang, SI Lin, YH Pan, LD Lin, YL Wang, SY Yeung, HH Chang, JH Jeng IL-1β-induced ICAM-1 and IL-8 expression/secretion of dental pulp cells is differentially regulated by IRAK and p38 J. Formos. Med. Assoc., 2018-12-14;118(8):1247-1254. 2018-12-14 [PMID: 30558829] (Human)
• H Leeman, E Kaminska, D Green, M Bodman-Smi, A Gravett, K Bodman-Smi, J Copier, G Coulton, A Fusi, AG Dalgleish Serum Apolipoprotein E and Other Inflammatory Markers Can Identify Non-Responding Patients to a Dendritic Cell Vaccine Transl Oncol, 2018-12-08;12(3):397-403. 2018-12-08 [PMID: 30530187] (Human)
• A Bokslag, C Franssen, LJ Alma, I Kovacevic, FV Kesteren, PW Teunissen, O Kamp, W Ganzevoort, PL Hordijk, CJM Groot, WJ Paulus Early-onset preeclampsia predisposes to preclinical diastolic left ventricular dysfunction in the fifth decade of life: An observational study PLoS ONE, 2018-06-12;13(6):e0198908. 2018-06-12 [PMID: 29894501] (Human)
• S Liong, R Lim, C Nguyen-Ngo, G Barker, HC Parkington, M Lappas The immunoproteasome inhibitor ONX-0914 regulates inflammation and expression of contraction associated proteins in myometrium Eur. J. Immunol., 2018-06-28;0(0):. 2018-06-28 [PMID: 29883518] (Human)
• A Rundblad, KB Holven, I Bruheim, MC Myhrstad, SM Ulven Effects of fish and krill oil on gene expression in peripheral blood mononuclear cells and circulating markers of inflammation: a randomised controlled trial J Nutr Sci, 2018-03-21;7(0):e10. 2018-03-21 [PMID: 29599972] (Human)
• JK Wright, K Hayford, V Tran, GM Al Kibria, A Baqui, A Manajjir, A Mahmud, N Begum, M Siddiquee, KC Kain, A Farzin Biomarkers of endothelial dysfunction predict sepsis mortality in young infants: a matched�case-control study BMC Pediatr, 2018-03-23;18(1):118. 2018-03-23 [PMID: 29571293] (Human)
• M Oleszowsky, MF Seidel Serum Soluble Vascular Cell Adhesion Molecule-1 Overexpression Is a Disease Marker in Patients with First-Time Diagnosed Antinuclear Antibodies: A Prospective, Observational Pilot Study Biomed Res Int, 2018-02-01;2018(0):8286067. 2018-02-01 [PMID: 29487871] (Human)
• A Paunel-Gör, M Wacker, M El Aita, S Hassan, G Schlachten, A Deppe, YH Choi, E Kuhn, TO Mehler, T Wahlers cfDNA correlates with endothelial damage after cardiac surgery with prolonged cardiopulmonary bypass and amplifies NETosis in an intracellular TLR9-independent manner Sci Rep, 2017-12-12;7(1):17421. 2017-12-12 [PMID: 29234042] (Human)
• ME Soto, JL Salas, J Vargas-Bar, R Marquez, A Rodriguez-, R Bojalil-Pa, I Pérez-Torr, V Guarner-La Pre- and post-surgical evaluation of the inflammatory response in patients with aortic stenosis treated with different types of prosthesis BMC Cardiovasc Disord, 2017-04-14;17(1):100. 2017-04-14 [PMID: 28410571] (Human)
• M Du, A Martin, F Hays, J Johnson, RA Farjo, KM Farjo Serum retinol-binding protein-induced endothelial inflammation is mediated through the activation of toll-like receptor 4 Mol. Vis., 2017-03-31;23(0):185-197. 2017-03-31 [PMID: 28400700] (Human)
• E Fernlund, T Gyllenhamm, R Jablonowsk, M Carlsson, A Larsson, J Ärnlöv, P Liuba Serum Biomarkers of Myocardial Remodeling and Coronary Dysfunction in Early Stages of Hypertrophic Cardiomyopathy in the Young Pediatr Cardiol, 2017-03-30;0(0):. 2017-03-30 [PMID: 28361263] (Human)
• V Selvarani, S Roland, S Andy, H James Prevention of influenza virus induced bacterial superinfection by standardized Echinacea purpurea, via regulation of surface receptor expression in human bronchial epithelial cells Virus Res, 2017-03-07;0(0):. 2017-03-07 [PMID: 28279802] (Human)
• Serum from Varicose Patients Induces Senescence-Related Dysfunction of Vascular Endothelium Generating Local and Systemic Proinflammatory Conditions Oxid Med Cell Longev, 2016-11-23;2016(0):2069290. 2016-11-23 [PMID: 27994710] (Human)
• ADAM17 substrate release in proximal tubule drives kidney fibrosis JCI Insight, 2016-08-18;1(13):. 2016-08-18 [PMID: 27642633] (Human)
• Modulation of Endothelial Inflammation by Low and High Magnitude Cyclic Stretch PLoS ONE, 2016-04-29;11(4):e0153387. 2016-04-29 [PMID: 27128976] (Human)
• McDonald C, Darling A, Conroy A, Tran V, Cabrera A, Liles W, Wang M, Aboud S, Urassa W, Fawzi W, Kain K Inflammatory and Angiogenic Factors at Mid-Pregnancy Are Associated with Spontaneous Preterm Birth in a Cohort of Tanzanian Women. PLoS ONE, 2015-08-06;10(8):e0134619. 2015-08-06 [PMID: 26247200] (Human)
• Kearney C, Cullen S, Tynan G, Henry C, Clancy D, Lavelle E, Martin S Necroptosis suppresses inflammation via termination of TNF- or LPS-induced cytokine and chemokine production. Cell Death Differ, 2015-01-23;22(8):1313-27. 2015-01-23 [PMID: 25613374] (Human)
• Greulich T, Nell C, Koepke J, Fechtel J, Franke M, Schmeck B, Haid D, Apelt S, Filipovic S, Kenn K, Janciauskiene S, Vogelmeier C, Koczulla A Benefits of whole body vibration training in patients hospitalised for COPD exacerbations - a randomized clinical trial. BMC Pulm Med, 2014-04-11;14(0):60. 2014-04-11 [PMID: 24725369] (Human)
• Conroy A, Gelvez M, Hawkes M, Rajwans N, Liles W, Villar-Centeno L, Kain K Host biomarkers distinguish dengue from leptospirosis in Colombia: a case-control study. BMC Infect Dis, 2014-01-20;14(0):35. 2014-01-20 [PMID: 24444080] (Human)
• Vasquez A, Segura C, Blair S Induction of pro-inflammatory response of the placental trophoblast by Plasmodium falciparum infected erythrocytes and TNF. Malar J, 2013-11-15;12(0):421. 2013-11-15 [PMID: 24237643] (Human)
• Fan , Yuqi, Wang , Lianshen, Li , Yuanmin, Yin , Zhaofang, Xu , Zuojun, Wang , Changqia Quantification of endothelial microparticles on modified cytometric bead assay and prognosis in chest pain patients. Circ J, 2013-11-08;78(1):206-14. 2013-11-08 [PMID: 24200872] (Human)
• Erdman LK, Dhabangi A, Musoke C, Conroy AL, Hawkes M, Higgins S, Rajwans N, Wolofsky KT, Streiner DL, Liles WC, Cserti-Gazdewich CM, Kain KC Combinations of Host Biomarkers Predict Mortality among Ugandan Children with Severe Malaria: A Retrospective Case-Control Study. PLoS ONE, 2011-02-25;6(2):e17440. 2011-02-25 [PMID: 21364762] (Human)
• Conroy AL, Phiri H, Hawkes M, Glover S, Mallewa M, Seydel KB, Taylor TE, Molyneux ME, Kain KC Endothelium-based biomarkers are associated with cerebral malaria in Malawian children: a retrospective case-control study. PLoS ONE, 2010-12-29;5(12):e15291. 2010-12-29 [PMID: 21209923] (Human)
• Hawn TR, Scholes D, Wang H, Li SS, Stapleton AE, Janer M, Aderem A, Stamm WE, Zhao LP, Hooton TM Genetic variation of the human urinary tract innate immune response and asymptomatic bacteriuria in women. PLoS ONE, 2009-12-15;4(12):e8300. 2009-12-15 [PMID: 20016852] (Human)
• Suri SS, Rakotondradany F, Myles AJ, Fenniri H, Singh B The role of RGD-tagged helical rosette nanotubes in the induction of inflammation and apoptosis in human lung adenocarcinoma cells through the P38 MAPK pathway. Biomaterials, 2009-02-27;30(17):3084-90. 2009-02-27 [PMID: 19250666] (Human)
• Hsu HY, Wittemann S, Schneider EM, Weiss M, Joos TO Suspension microarrays for the identification of the response patterns in hyperinflammatory diseases. Med Eng Phys, 2008-03-03;30(8):976-83. 2008-03-03 [PMID: 18313970] (Human)

FAQs for ICAM-1/CD54 (DY720). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: ICAM1