Human Angiopoietin-2 DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human Angiopoietin-2 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: ANGPT2

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Angiopoietin-2 DuoSet ELISA, 15 Plate

• AGPT2
• ANG2
• ANG-2
• angiopoietin 2
• Angiopoietin-2
• angiopoietin-2a
• angiopoietin-2B
• angiopoitin 2
• ANGPT2
• Tie2-ligand

Background

Angiopoietins, Ang-1, Ang-2, and Ang-3 (mouse)/Ang-4 (human), are natural agonists or antagonists of the Tie-2 receptor tyrosine kinase and are important modulators of angiogenesis. Two domains characterize the angiopoietin family of proteins: an N-terminal coiled-coil domain that mediates homo-oligomerization, and a C-terminal fibrinogen-like domain that binds Tie-2.

Publications for Angiopoietin-2 (DY623)(39)

Publications using DY623

• Mayhew, JA;Witten, AJ;Bond, CA;Opoka, RO;Bangirana, P;Conroy, AL;Hernandez-Alvarado, N;Schleiss, MR;John, CC; Cytomegalovirus reactivation and acute and chronic complications in children with cerebral malaria: a prospective cohort study Malaria journal 2025-02-17 [PMID: 39962580] (Human)
• Korde, A;Haslip, M;Pednekar, P;Khan, A;Chioccioli, M;Mehta, S;Lopez-Giraldez, F;Bermejo, S;Rojas, M;Dela Cruz, C;Matthay, MA;Pober, JS;Pierce, RW;Takyar, SS; MicroRNA-1 protects the endothelium in acute lung injury JCI insight 2023-09-22 [PMID: 37737266] (Human)
• S Macdonald, E Bosio, NI Shapiro, L Balmer, S Burrows, M Hibbs, T Jowitt, L Smart, G Arendts, D Fatovich No association between intravenous fluid volume and endothelial glycocalyx shedding in patients undergoing resuscitation for sepsis in the emergency department Scientific Reports, 2022-05-24;12(1):8733. 2022-05-24 [PMID: 35610344] (Human)
• M Reijrink, J van Ark, CPH Lexis, LM Visser, ME Lodewijk, ICC van der Ho, CJ Zeebregts, H van Goor, SCA de Jager, G Pasterkamp, BHR Wolffenbut, JL Hillebrand Increased frequency of proangiogenic tunica intima endothelial kinase 2 (Tie2) expressing monocytes in individuals with type 2 diabetes mellitus Cardiovascular Diabetology, 2022-05-12;21(1):72. 2022-05-12 [PMID: 35549955] (Human)
• J Villar, R Herrán-Mon, E González-H, M Prieto-Gon, A Ambrós, A Rodríguez-, A Muriel-Bom, R Solano, C Cuenca-Rub, A Vidal, C Flores, JM González-M, MI García-Lao, Genetics o Clinical and biological markers for predicting ARDS and outcome in septic patients Scientific Reports, 2021-11-22;11(1):22702. 2021-11-22 [PMID: 34811434] (Human)
• NV Bogatcheva, ME Coleman Concentrated Secretome of Adipose Stromal Cells Limits Influenza A Virus-Induced Lung Injury in Mice Cells, 2021-03-24;10(4):. 2021-03-24 [PMID: 33804896] (Human)
• H Dai, O Penack, A Radujkovic, D Schult, J Majer-Laut, IW Blau, L Bullinger, S Jiang, C Müller-Tid, P Dreger, T Luft Early bilirubinemia after allogeneic stem cell transplantation-an endothelial complication Bone marrow transplantation, 2021-01-30;0(0):. 2021-01-30 [PMID: 33517355] (Human)
• BJ Ouma, P Bangirana, JM Ssenkusu, D Datta, RO Opoka, R Idro, KC Kain, CC John, AL Conroy Plasma angiopoietin-2 is associated with age-related deficits in cognitive sub-scales in Ugandan children following severe malaria Malaria Journal, 2021-01-06;20(1):17. 2021-01-06 [PMID: 33407493] (Human)
• HK Pihlstrøm, T Ueland, AE Michelsen, P Aukrust, F Gatti, C Hammarströ, M Kasprzycka, J Wang, G Haraldsen, G Mjøen, DO Dahle, K Midtvedt, IA Eide, A Hartmann, H Holdaas Exploring the potential effect of paricalcitol on markers of inflammation in de novo renal transplant recipients PLoS ONE, 2020-12-16;15(12):e0243759. 2020-12-16 [PMID: 33326471] (Human)
• J Ohmes, Y Xiao, F Wang, MD Mikkelsen, TT Nguyen, H Schmidt, A Seekamp, AS Meyer, S Fuchs Effect of Enzymatically Extracted Fucoidans on Angiogenesis and Osteogenesis in Primary Cell Culture Systems Mimicking Bone Tissue Environment Mar Drugs, 2020-09-21;18(9):. 2020-09-21 [PMID: 32967359] (Human)
• A Chatterjee, R Eshwaran, H Huang, D Zhao, M Schmidt, T Wieland, Y Feng Role of the Ang2-Tie2 Axis in Vascular Damage Driven by High Glucose or Nucleoside Diphosphate Kinase B Deficiency Int J Mol Sci, 2020-05-25;21(10):. 2020-05-25 [PMID: 32466219] (Human)
• PP Singh, S Bhandari, RK Sharma, N Singh, PK Bharti Association of Angiopoietin Dysregulation in Placental Malaria with Adverse Birth Outcomes Dis. Markers, 2020-01-13;2020(0):6163487. 2020-01-13 [PMID: 32399088] (Human)
• A Merz, U Germing, G Kobbe, J Kaivers, A Jauch, A Radujkovic, M Hummel, A Benner, M Merz, P Dreger, T Luft EASIX for prediction of survival in lower-risk myelodysplastic syndromes Blood Cancer J, 2019-11-11;9(11):85. 2019-11-11 [PMID: 31712595] (Human)
• L Tiainen, EA Korhonen, VM Leppänen, T Luukkaala, M Hämäläinen, M Tanner, O Lahdenperä, P Vihinen, A Jukkola, P Karihtala, S Aho, E Moilanen, K Alitalo, PL Kellokumpu High baseline Tie1 level predicts poor survival in metastatic breast cancer BMC Cancer, 2019-07-24;19(1):732. 2019-07-24 [PMID: 31340773] (Human)
• H Murata, T Tsuzuki, T Kido, M Kakita-Kob, N Kida, Y Hisamatsu, H Okada Progestin-induced heart and neural crest derivatives-expressed transcript 2 inhibits angiopoietin 2 via fibroblast growth factor 9 in human endometrial stromal cells Reprod Biol, 2019-03-06;0(0):. 2019-03-06 [PMID: 30852242] (Human)
• MMS Chaves, MS Mendes, MP Schwermann, R Queiroz, RF Coelho, FTS Salmito, GC Meneses, AMC Martins, ACOM Moreira, AB Libório Angiopoietin-2: A Potential Mediator of the Glycocalyx Injury in Adult Nephrotic Patients J Clin Med, 2018-10-31;7(11):. 2018-10-31 [PMID: 30384404] (Human)
• J Bourland, J Fradette, FA Auger Tissue-engineered 3D melanoma model with blood and lymphatic capillaries for drug development Sci Rep, 2018-09-04;8(1):13191. 2018-09-04 [PMID: 30181613] (Human)
• JK Wright, K Hayford, V Tran, GM Al Kibria, A Baqui, A Manajjir, A Mahmud, N Begum, M Siddiquee, KC Kain, A Farzin Biomarkers of endothelial dysfunction predict sepsis mortality in young infants: a matched�case-control study BMC Pediatr, 2018-03-23;18(1):118. 2018-03-23 [PMID: 29571293] (Human)
• E Shabani, B Hanisch, RO Opoka, T Lavstsen, CC John Plasmodium falciparum EPCR-binding PfEMP1 expression increases with malaria disease severity and is elevated in retinopathy negative cerebral malaria BMC Med, 2017-10-13;15(1):183. 2017-10-13 [PMID: 29025399] (Human)
• J Retzlaff, K Thamm, CC Ghosh, W Ziegler, H Haller, SM Parikh, S David Flunarizine suppresses endothelial Angiopoietin-2 in a calcium - dependent fashion in sepsis Sci Rep, 2017-03-09;7(0):44113. 2017-03-09 [PMID: 28276491] (Human)
• Estela Shabani The endothelial protein C receptor rs867186-GG genotype is associated with increased soluble EPCR and could mediate protection against severe malaria Sci Rep, 2016-06-03;6(0):27084. 2016-06-03 [PMID: 27255786] (Human)
• Hawkes M, Conroy A, Opoka R, Hermann L, Thorpe K, McDonald C, Kim H, Higgins S, Namasopo S, John C, Miller C, Liles W, Kain K Inhaled nitric oxide as adjunctive therapy for severe malaria: a randomized controlled trial. Malar J, 2015-10-29;14(1):421. 2015-10-29 [PMID: 26510464] (Human)
• McDonald C, Darling A, Conroy A, Tran V, Cabrera A, Liles W, Wang M, Aboud S, Urassa W, Fawzi W, Kain K Inflammatory and Angiogenic Factors at Mid-Pregnancy Are Associated with Spontaneous Preterm Birth in a Cohort of Tanzanian Women. PLoS ONE, 2015-08-06;10(8):e0134619. 2015-08-06 [PMID: 26247200] (Human)
• Ataide R, Murillo O, Dombrowski J, Souza R, Lima F, Lima G, Hristov A, Valle S, Di Santi S, Epiphanio S, Marinho C Malaria in Pregnancy Interacts with and Alters the Angiogenic Profiles of the Placenta. PLoS Negl Trop Dis, 2015-06-19;9(6):e0003824. 2015-06-19 [PMID: 26090803] (Human)
• Kim Aubin, Caroline Vincent, Maryse Proulx, Dominique Mayrand, Julie Fradette Creating capillary networks within human engineered tissues: Impact of adipocytes and their secretory products Acta Biomaterialia 2015-01-01 [PMID: 25278444] (Human)
• Conroy A, Gelvez M, Hawkes M, Rajwans N, Liles W, Villar-Centeno L, Kain K Host biomarkers distinguish dengue from leptospirosis in Colombia: a case-control study. BMC Infect Dis, 2014-01-20;14(0):35. 2014-01-20 [PMID: 24444080] (Human)
• Deleuze V, El-Hajj R, Chalhoub E, Dohet C, Pinet V, Couttet P, Mathieu D Angiopoietin-2 Is a Direct Transcriptional Target of TAL1, LYL1 and LMO2 in Endothelial Cells. PLoS ONE, 2012-07-06;7(7):e40484. 2012-07-06 [PMID: 22792348] (Human)
• Highly invasive melanoma cells activate the vascular endothelium via an MMP-2/integrin alphavbeta5-induced secretion of VEGF-A. Am. J. Pathol., 2012-05-31;181(2):693-705. 2012-05-31 [PMID: 22659470] (Human)
• Conroy AL, Liles WC, Molyneux ME Performance characteristics of combinations of host biomarkers to identify women with occult placental malaria: a case-control study from Malawi. PLoS ONE, 2011-12-12;6(12):e28540. 2011-12-12 [PMID: 22174834] (Human)
• Erdman LK, Dhabangi A, Musoke C, Conroy AL, Hawkes M, Higgins S, Rajwans N, Wolofsky KT, Streiner DL, Liles WC, Cserti-Gazdewich CM, Kain KC Combinations of Host Biomarkers Predict Mortality among Ugandan Children with Severe Malaria: A Retrospective Case-Control Study. PLoS ONE, 2011-02-25;6(2):e17440. 2011-02-25 [PMID: 21364762] (Human)
• Conroy AL, Phiri H, Hawkes M, Glover S, Mallewa M, Seydel KB, Taylor TE, Molyneux ME, Kain KC Endothelium-based biomarkers are associated with cerebral malaria in Malawian children: a retrospective case-control study. PLoS ONE, 2010-12-29;5(12):e15291. 2010-12-29 [PMID: 21209923] (Human)
• Koenecke C, Kumpers P, Lukasz A, Dammann E, Verhagen W, Gohring G, Buchholz S, Krauter J, Eder M, Schlegelberger B, Ganser A Shedding of the endothelial receptor tyrosine kinase Tie2 correlates with leukemic blast burden and outcome after allogeneic hematopoietic stem cell transplantation for AML. Ann. Hematol., 2010-05-01;89(5):459-67. 2010-05-01 [PMID: 20054548] (Human)
• Bolin M, Wiberg-Itzel E, Wikstrom AK, Goop M, Larsson A, Olovsson M, Akerud H Angiopoietin-1/angiopoietin-2 ratio for prediction of preeclampsia. Am. J. Hypertens., 2009-05-28;22(8):891-5. 2009-05-28 [PMID: 19478794] (Human)
• Szarvas T, Jager T, Droste F, Becker M, Kovalszky I, Romics I, Ergun S, Rubben H Serum levels of angiogenic factors and their prognostic relevance in bladder cancer. Pathol. Oncol. Res., 2008-09-20;15(2):193-201. 2008-09-20 [PMID: 18807212] (Human)
• van der Heijden M, van Nieuw Amerongen GP, Koolwijk P, van Hinsbergh VW, Groeneveld AB Angiopoietin-2, permeability oedema, occurrence and severity of ALI/ARDS in septic and non-septic critically ill patients. Thorax, 2008-06-17;63(10):903-9. 2008-06-17 [PMID: 18559364] (Human)
• Orfanos SE, Kotanidou A, Glynos C, Athanasiou C, Tsigkos S, Dimopoulou I, Sotiropoulou C, Zakynthinos S, Armaganidis A, Papapetropoulos A, Roussos C Angiopoietin-2 is increased in severe sepsis: correlation with inflammatory mediators. Crit. Care Med., 2007-01-01;35(1):199-206. 2007-01-01 [PMID: 17110873] (Bovine, Human)
• Kalomenidis I, Kollintza A, Sigala I, Papapetropoulos A, Papiris S, Light RW, Roussos C Angiopoietin-2 levels are elevated in exudative pleural effusions. Chest, 2006-05-01;129(5):1259-66. 2006-05-01 [PMID: 16685017] (Human)
• Tsigkos S, Zhou Z, Kotanidou A, Fulton D, Zakynthinos S, Roussos C, Papapetropoulos A Regulation of Ang2 release by PTEN/PI3-kinase/Akt in lung microvascular endothelial cells. J. Cell. Physiol., 2006-05-01;207(2):506-11. 2006-05-01 [PMID: 16447257] (Bovine)
• Caine GJ, Blann AD, Stonelake PS, Ryan P, Lip GY Plasma angiopoietin-1, angiopoietin-2 and Tie-2 in breast and prostate cancer: a comparison with VEGF and Flt-1. Eur. J. Clin. Invest., 2003-10-01;33(10):883-90. 2003-10-01 [PMID: 14511360] (Human)

FAQs for Angiopoietin-2 (DY623). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: ANGPT2