Human Amphiregulin Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human Amphiregulin Quantikine ELISA Kit Summary

• Background: The Quantikine Human Amphiregulin Immunoassay is a 4.5 hour solid phase ELISA designed to measure human Amphiregulin in cell culture supernates, cell lysates serum, plasma, saliva, and urine. It contains E. coli-derived recombinant human Amphiregulin and has been shown to accurately quantitate the recombinant factor. Results obtained with natural human Amphiregulin showed ...linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Amphiregulin.
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• Specificity: Recombinant and natural human Amphiregulin
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: AREG

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Amphiregulin Quantikine ELISA Kit

• Amphiregulin
• AR
• AREG
• AREGB
• Colorectum cell-derived growth factor
• CRDGF
• MGC13647
• schwannoma-derived growth factor
• SDGF

Background

Amphiregulin (AR) is an EGF family growth factor that is released as a soluble protein following proteolytic cleavage of its transmembrane precursor. Amphiregulin is expressed by numerous carcinoma cell lines and epithelial cells of the colon, stomach, breast, ovary and kidney. It acts through ErbB family of receptor to stimulate the proliferation of keratinocytes, mammary epithelial cells, fibroblasts, astrocytes and glial cells. Amphiregulin can also inhibit the growth of certain tumor cells.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Amphiregulin (DAR00)(18)

Publications using DAR00

• Das, A;Ariyakumar, G;Gupta, N;Kamdar, S;Barugahare, A;Deveson-Lucas, D;Gee, S;Costeloe, K;Davey, MS;Fleming, P;Gibbons, DL; Identifying immune signatures of sepsis to increase diagnostic accuracy in very preterm babies Nature communications 2024-01-09 [PMID: 38195661] (Human)
• Kamentseva, RS;Kharchenko, MV;Gabdrahmanova, GV;Kotov, MA;Kosheverova, VV;Kornilova, ES; EGF, TGF-? and Amphiregulin Differently Regulate Endometrium-Derived Mesenchymal Stromal/Stem Cells International journal of molecular sciences 2023-08-29 [PMID: 37686213] (Human)
• Y Liu, Y Zhong, X Shen, X Guo, R Wu, T Yang, M Chen Luteinizing hormone stimulates the expression of amphiregulin in human theca cells Journal of ovarian research, 2022-12-07;15(1):129. 2022-12-07 [PMID: 36476625] (Human)
• B Sieber, F Lu, SM Stribbling, AG Grieve, AJ Ryan, M Freeman iRhom2 regulates ERBB signalling to promote KRAS-driven tumour growth of lung cancer cells Journal of Cell Science, 2022-09-08;0(0):. 2022-09-08 [PMID: 35971826] (Human)
• C Vogeley, NC Sondermann, S Woeste, AA Momin, V Gilardino, F Hartung, M Heinen, SK Maa beta , M Mescher, M Pollet, KM Rolfes, CFA Vogel, A Rossi, D Lang, ST Arold, M Nakamura, T Haarmann-S Unraveling the differential impact of PAHs and dioxin-like compounds on AKR1C3 reveals the EGFR extracellular domain as a critical determinant of the AHR response Environment international, 2021-11-20;158(0):106989. 2021-11-20 [PMID: 34991250] (Human)
• SA Kim, H Park, KJ Kim, JW Kim, JH Sung, M Nam, JH Lee, EH Jung, KJ Suh, JY Lee, SH Kim, JO Lee, JW Kim, YJ Kim, JH Kim, SM Bang, JS Lee, KW Lee Amphiregulin can predict treatment resistance to palliative first-line cetuximab plus FOLFIRI chemotherapy in patients with RAS wild-type metastatic colorectal cancer Scientific Reports, 2021-12-10;11(1):23803. 2021-12-10 [PMID: 34893673] (Human)
• K Fukushima, N Itaba, Y Kono, S Okazaki, S Enokida, N Kuranobu, J Murakami, M Enokida, H Nagashima, S Kanzaki, N Namba, G Shiota Secreted matrix metalloproteinase-14 is a predictor for antifibrotic effect of IC-2-engineered mesenchymal stem cell sheets on liver fibrosis in mice Regenerative Therapy, 2021-08-26;18(0):292-301. 2021-08-26 [PMID: 34504910] (Human)
• IT Stancil, JE Michalski, D Davis-Hall, HW Chu, JA Park, CM Magin, IV Yang, BJ Smith, E Dobrinskik, DA Schwartz Pulmonary fibrosis distal airway epithelia are dynamically and structurally dysfunctional Nature Communications, 2021-07-27;12(1):4566. 2021-07-27 [PMID: 34315881] (Human)
• A Wiedemann, E Foucat, H Hocini, C Lefebvre, BP Hejblum, M Durand, M Krüger, AK Keita, A Ayouba, S Mély, JC Fernandez, A Touré, S Fourati, C Lévy-March, H Raoul, E Delaporte, L Koivogui, R Thiébaut, C Lacabaratz, Y Lévy Long-lasting severe immune dysfunction in Ebola virus disease survivors Nat Commun, 2020-07-24;11(1):3730. 2020-07-24 [PMID: 32709840] (Human)
• L Wang, L Wang, H Zhang, J Lu, Z Zhang, H Wu, Z Liang AREG mediates the epithelial?mesenchymal transition in pancreatic cancer cells via the EGFR/ERK/NF?&kappaB signalling pathway Oncol. Rep., 2020-02-27;0(0):. 2020-02-27 [PMID: 32323797] (Human)
• Q Xu, Q Long, D Zhu, D Fu, B Zhang, L Han, M Qian, J Guo, J Xu, L Cao, YE Chin, JP Coppé, EW Lam, J Campisi, Y Sun Targeting amphiregulin (AREG) derived from senescent stromal cells diminishes cancer resistance and averts programmed cell death 1 ligand (PD-L1)-mediated immunosuppression Aging Cell, 2019-09-07;0(0):e13027. 2019-09-07 [PMID: 31493351] (Human)
• J Jia, C Li, J Yang, X Wang, R Li, S Luo, Z Li, J Liu, Z Liu, Y Zheng Yes-associated protein promotes the abnormal proliferation of psoriatic keratinocytes via an amphiregulin dependent pathway Sci Rep, 2018-10-15;8(1):14513. 2018-10-15 [PMID: 30323299] (Human)
• P Mésange, A Bouygues, N Ferrand, M Sabbah, AE Escargueil, A Savina, B Chibaudel, C Tournigand, T Andre, A de Gramont, AK Larsen Combinations of Bevacizumab and Erlotinib show activity in colorectal cancer independent of RAS status Clin. Cancer Res., 2018-02-28;0(0):. 2018-02-28 [PMID: 29490990] (Human)
• D Alvarado, GF Ligon, JS Lillquist, SB Seibel, G Wallweber, VM Neumeister, DL Rimm, G McMahon, TM LaVallee ErbB activation signatures as potential biomarkers for anti-ErbB3 treatment in HNSCC PLoS ONE, 2017-07-19;12(7):e0181356. 2017-07-19 [PMID: 28723928] (Human)
• Late acute graft versus host disease: a prospective analysis of clinical outcomes and circulating angiogenic factors Blood, 2016-09-13;0(0):. 2016-09-13 [PMID: 27625357] (Human)
• Trussoni C, Tabibian J, Splinter P, O'Hara S Lipopolysaccharide (LPS)-Induced Biliary Epithelial Cell NRas Activation Requires Epidermal Growth Factor Receptor (EGFR). PLoS ONE, 2015-04-27;10(4):e0125793. 2015-04-27 [PMID: 25915403] (Human)
• Yonesaka , Kimio, Satoh , Taroh, Ueda , Shinya, Yoshida , Takeshi, Takeda , Masayuki, Shimizu , Toshio, Okamoto , Isamu, Nishio , Kazuto, Tamura , Takao, Nakagawa , Kazuhiko Circulating hepatocyte growth factor is correlated with resistance to cetuximab in metastatic colorectal cancer. Anticancer Res, 2015-03-01;35(3):1683-9. 2015-03-01 [PMID: 25750328] (Human)
• Zhang Z, Dong Z, Lauxen I, Filho M, Nor J Endothelial cell-secreted EGF induces epithelial to mesenchymal transition and endows head and neck cancer cells with stem-like phenotype. Cancer Res, 2014-03-31;74(10):2869-81. 2014-03-31 [PMID: 24686166] (Human)

Bioinformatics

• Gene Symbol: AREG