Human Amphiregulin DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human Amphiregulin DuoSet ELISA, 15 Plate Summary

• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: AREG

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Amphiregulin DuoSet ELISA, 15 Plate

• Amphiregulin
• AR
• AREG
• AREGB
• Colorectum cell-derived growth factor
• CRDGF
• MGC13647
• schwannoma-derived growth factor
• SDGF

Background

Amphiregulin (AR) is an EGF family growth factor that is released as a soluble protein following proteolytic cleavage of its transmembrane precursor. Amphiregulin is expressed by numerous carcinoma cell lines and epithelial cells of the colon, stomach, breast, ovary and kidney. It acts through ErbB family of receptor to stimulate the proliferation of keratinocytes, mammary epithelial cells, fibroblasts, astrocytes and glial cells. Amphiregulin can also inhibit the growth of certain tumor cells.

Publications for Amphiregulin (DY262)(41)

Publications using DY262

• B Jaeger, JC Schupp, L Plappert, O Terwolbeck, N Artysh, G Kayser, P Engelhard, TS Adams, R Zweigerdt, H Kempf, S Lienenklau, W Garrels, I Nazarenko, D Jonigk, M Wygrecka, D Klatt, A Schambach, N Kaminski, A Prasse Airway basal cells show a dedifferentiated KRT17highPhenotype and promote fibrosis in idiopathic pulmonary fibrosis Nature Communications, 2022;13(1):5637. 2022 [PMID: 36163190] (Human)
• M Patankar, M Li, A Khalatbari, JD Castle, L Hu, C Zhang, A Shaker Inflammatory and Proliferative Pathway Activation in Human Esophageal Myofibroblasts Treated with Acidic Bile Salts International Journal of Molecular Sciences, 2022;23(18):. 2022 [PMID: 36142285] (Human)
• J Villar, R Herrán-Mon, E González-H, M Prieto-Gon, A Ambrós, A Rodríguez-, A Muriel-Bom, R Solano, C Cuenca-Rub, A Vidal, C Flores, JM González-M, MI García-Lao, Genetics o Clinical and biological markers for predicting ARDS and outcome in septic patients Scientific Reports, 2021;11(1):22702. 2021 [PMID: 34811434] (Human)
• S Ghosh, NB Nataraj, A Noronha, S Patkar, A Sekar, S Mukherjee, S Winograd-K, L Kramarski, A Verma, M Lindzen, DD Garcia, J Green, G Eisenberg, H Gil-Henn, A Basu, Y Lender, S Weiss, M Oren, M Lotem, B Geiger, E Ruppin, Y Yarden PD-L1 recruits phospholipase C and enhances tumorigenicity of lung tumors harboring mutant forms of EGFR Cell Reports, 2021;35(8):109181. 2021 [PMID: 34038737] (Human)
• M Lindzen, S Ghosh, A Noronha, D Drago, NB Nataraj, O Leitner, S Carvalho, E Zmora, S Sapoznik, KB Shany, K Levanon, D Aderka, BS Ramírez, M Dahlhoff, I McNeish, Y Yarden Targeting autocrine amphiregulin robustly and reproducibly inhibits ovarian cancer in a syngeneic model: roles for wildtype p53 Oncogene, 2021;0(0):. 2021 [PMID: 33941851] (Human)
• C Schiffers, C van de Wet, RA Bauer, A Habibovic, M Hristova, CM Dustin, S Lambrichts, PM Vacek, EF Wouters, NL Reynaert, A van der Vl Downregulation of epithelial DUOX1 in Chronic Obstructive Pulmonary Disease contributes to disease pathogenesis JCI Insight, 2020;0(0):. 2020 [PMID: 33301419] (Human)
• SJ Wang, R Li, TSC Ng, G Luthria, MJ Oudin, M Prytyskach, RH Kohler, R Weissleder, DA Lauffenbur, MA Miller Efficient blockade of locally reciprocated tumor-macrophage signaling using a TAM-avid nanotherapy Sci Adv, 2020;6(21):eaaz8521. 2020 [PMID: 32494745] (Human)
• JH Ko, HJ Kim, HJ Jeong, HJ Lee, JY Oh Mesenchymal Stem and Stromal Cells Harness Macrophage-Derived Amphiregulin to Maintain Tissue Homeostasis Cell Rep, 2020;30(11):3806-3820.e6. 2020 [PMID: 32187551] (Human)
• A Sampera, FJ Sánchez-Ma, O Arpí, L Visa, M Iglesias, S Menéndez, É Gaye, A Dalmases, S Clavé, M Gelabert-B, TT Poulsen, M Kragh, B Bellosillo, J Albanell, A Rovira, C Montagut HER-family ligands promote acquired resistance to trastuzumab in gastric cancer Mol. Cancer Ther., 2019;0(0):. 2019 [PMID: 31484705] (Human)
• J Ho, X Yang, SA Nikou, N Kichik, A Donkin, NO Ponde, JP Richardson, RL Gratacap, LS Archambaul, CP Zwirner, C Murciano, R Henley-Smi, S Thavaraj, CJ Tynan, SL Gaffen, B Hube, RT Wheeler, DL Moyes, JR Naglik Candidalysin activates innate epithelial immune responses via epidermal growth factor receptor Nat Commun, 2019;10(1):2297. 2019 [PMID: 31127085] (Human)
• AJ Lam, KN MacDonald, AM Pesenacker, SC Juvet, KA Morishita, B Bressler, , JG Pan, SS Sidhu, JD Rioux, MK Levings Innate Control of Tissue-Reparative Human Regulatory T Cells J. Immunol., 2019;0(0):. 2019 [PMID: 30850479] (Human)
• SP Kubli, C Bassi, C Roux, A Wakeham, C Göbl, W Zhou, SM Jafari, B Snow, L Jones, L Palomero, KL Thu, L Cassetta, D Soong, T Berger, P Ramachandr, SP Baniasadi, G Duncan, M Lindzen, Y Yarden, C Herranz, C Lazaro, MF Chu, J Haight, P Tinto, J Silvester, DW Cescon, A Petit, S Pettersson, JW Pollard, TW Mak, MA Pujana, P Cappello, C Gorrini AhR controls redox homeostasis and shapes the tumor microenvironment in BRCA1-associated breast cancer Proc. Natl. Acad. Sci. U.S.A., 2019;0(0):. 2019 [PMID: 30733286] (Human)
• S Schmidt, N Schumacher, J Schwarz, S Tangermann, L Kenner, M Schlederer, M Sibilia, M Linder, A Altendorf-, T Knösel, ES Gruber, G Oberhuber, J Bolik, A Rehman, A Sinha, J Lokau, P Arnold, AS Cabron, F Zunke, C Becker-Pau, A Preaudet, P Nguyen, J Huynh, S Afshar-Ste, AL Chand, J Westermann, PJ Dempsey, C Garbers, D Schmidt-Ar, P Rosenstiel, T Putoczki, M Ernst, S Rose-John ADAM17 is required for EGF-R-induced intestinal tumors via IL-6 trans-signaling J. Exp. Med., 2018;0(0):. 2018 [PMID: 29472497] (Mouse)
• TM Nordgren, KL Bailey, AJ Heires, D Katafiasz, DJ Romberger Effects of agricultural organic dusts on human lung-resident mesenchymal stem (stromal) cell function Toxicol. Sci., 2018;0(0):. 2018 [PMID: 29319804] (Human)
• Seyyed Mortaza Haghgoo Prognostic value of rare and complex mutations in EGFR and serum levels of soluble EGFR and its ligands in Iranian non-small cell lung carcinoma patients Clin. Biochem, 2016;0(0):. 2016 [PMID: 27923629] (Human)
• Epidermal growth factor (EGF) receptor-ligand based molecular staging predicts prognosis in head and neck squamous cell carcinoma partly due to deregulated EGF- induced amphiregulin expression J Exp Clin Cancer Res, 2016;35(1):151. 2016 [PMID: 27669890] (Human)
• ADAM17 substrate release in proximal tubule drives kidney fibrosis JCI Insight, 2016;1(13):. 2016 [PMID: 27642633] (Human)
• Local Epidermal Growth Factor Receptor Signaling Mediates the Systemic Pathogenic Effects of Staphylococcus aureus Toxic Shock Syndrome PLoS ONE, 2016;11(7):e0158969. 2016 [PMID: 27414801] (Human)
• Aderhold C, Faber A, Umbreit C, Birk R, Weiss C, Sommer J, Hormann K, Schultz J Targeting mTOR and AREG with everolimus, sunitinib and sorafenib in HPV-positive and -negative SCC. Anticancer Res, 2015;35(4):1951-9. 2015 [PMID: 25862847] (Human)
• Noguchi S, Saito A, Horie M, Mikami Y, Suzuki H, Morishita Y, Ohshima M, Abiko Y, Mattsson J, Konig H, Lohr M, Edlund K, Botling J, Micke P, Nagase T An integrative analysis of the tumorigenic role of TAZ in human non-small cell lung cancer. Clin Cancer Res, 2014;20(17):4660-72. 2014 [PMID: 24951773] (Human)
• Parnia S, Hamilton L, Puddicombe S, Holgate S, Frew A, Davies D Autocrine ligands of the epithelial growth factor receptor mediate inflammatory responses to diesel exhaust particles. Respir Res, 2014;15(0):22. 2014 [PMID: 24555532] (Human)
• Chen , Z, Chen , J, Gu , Y, Hu , C, Li , J-L, Lin , S, Shen , H, Cao , C, Gao , R, Li , J, Ha , P K, Kaye , F J, Griffin , J D, Wu , L Aberrantly activated AREG-EGFR signaling is required for the growth and survival of CRTC1-MAML2 fusion-positive mucoepidermoid carcinoma cells. Oncogene, 2014;33(29):3869-77. 2014 [PMID: 23975434] (Human)
• Tanizaki J, Okamoto I, Okabe T, Sakai K, Tanaka K, Hayashi H, Kaneda H, Takezawa K, Kuwata K, Yamaguchi H, Hatashita E, Nishio K, Nakagawa K Activation of HER family signaling as a mechanism of acquired resistance to ALK inhibitors in EML4-ALK-positive non-small cell lung cancer. Clin Cancer Res, 2012;18(22):6219-26. 2012 [PMID: 22843788] (Human)
• Breshears L, Schlievert P, Peterson M A disintegrin and metalloproteinase 17 (ADAM17) and epidermal growth factor receptor (EGFR) signaling drive the epithelial response to Staphylococcus aureus toxic shock syndrome toxin-1 (TSST-1). J Biol Chem, 2012;287(39):32578-87. 2012 [PMID: 22833676] (Human)
• Richards FM, Tape CJ, Jodrell DI, Murphy G Anti-Tumour Effects of a Specific Anti-ADAM17 Antibody in an Ovarian Cancer Model In Vivo. PLoS ONE, 2012;7(7):e40597. 2012 [PMID: 22792380] (Human)
• Yasumoto K, Yamada T, Kawashima A, Wang W, Li Q, Donev IS, Tacheuchi S, Mouri H, Yamashita K, Ohtsubo K, Yano S The EGFR ligands amphiregulin and heparin-binding egf-like growth factor promote peritoneal carcinomatosis in CXCR4-expressing gastric cancer. Clin. Cancer Res., 2011;17(11):3619-30. 2011 [PMID: 21482691] (Human)
• Hickinson DM, Marshall GB, Beran GJ Identification of biomarkers in human head and neck tumor cell lines that predict for in vitro sensitivity to gefitinib. Clin Transl Sci, 2009;2(3):183-92. 2009 [PMID: 20443891] (Human)
• Stoll SW, Johnson JL, Li Y, Rittie L, Elder JT Amphiregulin carboxy-terminal domain is required for autocrine keratinocyte growth. J. Invest. Dermatol., 2010;130(8):2031-40. 2010 [PMID: 20428186] (Human)
• Egloff AM, Rothstein ME, Seethala R, Siegfried JM, Grandis JR, Stabile LP Cross-talk between estrogen receptor and epidermal growth factor receptor in head and neck squamous cell carcinoma. Clin. Cancer Res., 2009;15(21):6529-40. 2009 [PMID: 19825947] (Human)
• Han SW, Oh DY, Im SA, Park SR, Lee KW, Song HS, Lee NS, Lee KH, Choi IS, Lee MH, Kim MA, Kim WH, Bang YJ, Kim TY Phase II study and biomarker analysis of cetuximab combined with modified FOLFOX6 in advanced gastric cancer. Br. J. Cancer, 2009;100(2):298-304. 2009 [PMID: 19127259] (Human)
• Inoue Y, Miyamoto S, Fukami T, Shirota K, Yotsumoto F, Kawarabayashi T Amphiregulin is much more abundantly expressed than transforming growth factor-alpha and epidermal growth factor in human follicular fluid obtained from patients undergoing in vitro fertilization-embryo transfer. Fertil. Steril., 2009;91(4):1035-41. 2009 [PMID: 18325497] (Human)
• Yotsumoto F, Yagi H, Suzuki SO, Oki E, Tsujioka H, Hachisuga T, Sonoda K, Kawarabayashi T, Mekada E, Miyamoto S Validation of HB-EGF and amphiregulin as targets for human cancer therapy. Biochem. Biophys. Res. Commun., 2007;365(3):555-61. 2007 [PMID: 18023415] (Human)
• Eckstein N, Servan K, Girard L, Cai D, von Jonquieres G, Jaehde U, Kassack MU, Gazdar AF, Minna JD, Royer HD Epidermal growth factor receptor pathway analysis identifies amphiregulin as a key factor for cisplatin resistance of human breast cancer cells. J. Biol. Chem., 2007;283(2):739-50. 2007 [PMID: 17942395] (Human)
• Moss ML, Bomar M, Liu Q, Sage H, Dempsey P, Lenhart PM, Gillispie PA, Stoeck A, Wildeboer D, Bartsch JW, Palmisano R, Zhou P The ADAM10 prodomain is a specific inhibitor of ADAM10 proteolytic activity and inhibits cellular shedding events. J. Biol. Chem., 2007;282(49):35712-21. 2007 [PMID: 17895248] (Human)
• Rumelhard M, Ramgolam K, Hamel R, Marano F, Baeza-Squiban A Expression and role of EGFR ligands induced in airway cells by PM2.5 and its components. Eur. Respir. J., 2007;30(6):1064-73. 2007 [PMID: 17804444] (Human)
• Lemos-Gonzalez Y, Rodriguez-Berrocal FJ, Cordero OJ, Gomez C, Paez de la Cadena M Alteration of the serum levels of the epidermal growth factor receptor and its ligands in patients with non-small cell lung cancer and head and neck carcinoma. Br. J. Cancer, 2007;96(10):1569-78. 2007 [PMID: 17453000] (Human)
• Zhou BB, Peyton M, He B, Liu C, Girard L, Caudler E, Lo Y, Baribaud F, Mikami I, Reguart N, Yang G, Li Y, Yao W, Vaddi K, Gazdar AF, Friedman SM, Jablons DM, Newton RC, Fridman JS, Minna JD, Scherle PA Targeting ADAM-mediated ligand cleavage to inhibit HER3 and EGFR pathways in non-small cell lung cancer. Cancer Cell, 2006;10(1):39-50. 2006 [PMID: 16843264] (Human)
• Janes KA, Gaudet S, Albeck JG, Nielsen UB, Lauffenburger DA, Sorger PK The response of human epithelial cells to TNF involves an inducible autocrine cascade. Cell, 2006;124(6):1225-39. 2006 [PMID: 16564013] (Human)
• Baginski TK, Dabbagh K, Satjawatcharaphong C, Swinney DC Cigarette smoke synergistically enhances respiratory mucin induction by proinflammatory stimuli. Am. J. Respir. Cell Mol. Biol., 2006;35(2):165-74. 2006 [PMID: 16543607] (Human)
• Miyamoto S, Hirata M, Yamazaki A, Kageyama T, Hasuwa H, Mizushima H, Tanaka Y, Yagi H, Sonoda K, Kai M, Kanoh H, Nakano H, Mekada E Heparin-binding EGF-like growth factor is a promising target for ovarian cancer therapy. Cancer Res., 2004;64(16):5720-7. 2004 [PMID: 15313912] (Human)
• Kansra S, Johnson JL Autocrine extracellular signal-regulated kinase (ERK) activation in normal human keratinocytes: metalloproteinase-mediated release of amphiregulin triggers signaling from ErbB1 to ERK. Mol. Biol. Cell, 2004;15(9):4299-309. 2004 [PMID: 15254267] (Human)

Bioinformatics

• Gene Symbol: AREG