HtrA3 Antibody - Azide and BSA Free

Western Blot: HtrA3 Antibody [NBP3-04967] - Western blot analysis of extracts of various cell lines, using HtrA3 antibody (NBP3-04967) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

HtrA3 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit HtrA3 Antibody - Azide and BSA Free (NBP3-04967) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 334-453 of human HTRA3 (NP_444272.1). SDRITRFLTEFQDKQIKDWKKRFIGIRMRTITPSLVDELKASNPDFPEVSSGIYVQEVAPNSPSQRGGIQDGDIIVKVNGRPLVDSSELQEAVLTESPLLLEVRRGNDDLLFSIAPEVVM
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HTRA3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.05% Proclin 300
• Purity: Affinity purified

Alternate Names for HtrA3 Antibody - Azide and BSA Free

• EC 3.4.21
• EC 3.4.21.-
• EC 3.4.21.108
• High-temperature requirement factor A3
• HTRA 3
• HtrA serine peptidase 3
• HTRA3
• Pregnancy-related serine protease
• probable serine protease HTRA3
• Prsp
• serine protease HTRA3
• Tasp

Background

HtrA3 belongs to the human high-temperature requirement factor A (HtrA) family. HtrA3 has two isoforms (long and short), which are produced by alternative splicing. Similar to HtrA1, HtrA3 binds to various TGF-beta proteins and inhibits the signaling of BMP-4, -2 and TGF-beta 1. HtrA3 does not inhibit signaling originated from a constitutively active BMP receptor, indicating that the inhibition occurs upstream of the cell surface receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: HTRA3