PDZD11 Antibody

Western blot shows lysates of MOLT‑4 human acute lymphoblastic leukemia cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human PDZD11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5977) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PDZD11 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

PDZD11 Antibody Summary

• Immunogen: E. coli-derived recombinant human PDZD11
  Met1-His140
  Accession # Q5EBL8
• Specificity: Detects human PDZD11 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human PDZD2 and recombinant mouse PDZD2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PDZD11
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PDZD11 Antibody

• AIPP1
• ATPase-interacting PDZ protein
• PDZ domain containing 11
• PDZ domain-containing protein 11
• PDZD11
• PDZK11
• PISP

Background

PDZD11 (PDZ domain-containing protein 11; also AIPP1a and PISP) is a 17 kDa cytosolic protein that bears resemblance to members of the MALS/VELIS family of proteins. It is ubiquitously expressed, and appears to target calcium and copper ATPases to basolateral cell membranes. Human PDZD11 is 140 amino acids (aa) in length. It contains but one PDZ domain (aa 47-129) that apparently interacts with the C-terminus of partner proteins. One alternative start site exists that is 32 aa upstream of the standard site. This generates the AIPP1b isoform. Full-length human PDZD11 shares 97% aa identity with mouse PDZD11.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDZD11
• Uniprot:
  • Q5EBL8()