Polycystin-1/PKD1 Antibody [Unconjugated]

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Polycystin‑1/PKD1 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Sheep Anti-Human Polycystin‑1/PKD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6270) at 10 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

Polycystin-1/PKD1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Polycystin-1/PKD1
Gly4142-Thr4303
Accession # P98161
Specificity
Detects human Polycystin-1/PKD1 in direct ELISAs.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
PKD1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Polycystin-1/PKD1 Antibody [Unconjugated]

  • Autosomal dominant polycystic kidney disease 1 protein
  • PBP
  • Pc-1
  • PKD1
  • polycystic kidney disease 1 (autosomal dominant)
  • polycystic kidney disease-associated protein
  • polycystin 1
  • Polycystin1
  • Polycystin-1
  • transient receptor potential cation channel, subfamily P, member 1
  • TRPP1

Background

Polycystin-1 (also PKD1) is a 500-550 kDa member of the polycystin family of proteins. It is expressed in renal tubule primary cilia, and the membrane region that forms adherens junctions. Polycystin-1 binds to polycystin-2, promoting its insertion into the cell membrane, and regulating its calcium channel activity. In conjunction with polycystin-2, it detects fluid flow and converts this information into calcium signals. It also exists in the ER, where it negatively modulates polycystin-2 mediated calcium release. Mature human polycystin-1 is a 4280 amino acid (aa), 11 transmembrane glycoprotein. Its N-terminal extracellular region (aa 24-3074) is highly modular, and contains a C-type lecin domain, multiple Leu-rich repeats and PKD domains, one GPS region and a PLAT, REJ, WSC and LDLR domain. The cytoplasmic C-terminus (aa 4107-4303) contains a polycystin-2 coiled-coil interaction domain (aa 4220-4251). A percentage of polycystin-1 undergoes autoproteolysis after Leu3048, generating a presumed N-terminal fragment of ~ 350-400 kDa, and a membrane-bound C-terminal fragment of 150 kDa. Over aa 4141-4303, human polycystin-1 shares 71% aa identity with mouse polycystin-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PKD1
Uniprot