HTRA2/Omi Antibody [Alexa Fluor™ Plus 594]

• Reactivity: Hu, Mu, Rt
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Alexa Fluor Plus 594

HTRA2/Omi Antibody [Alexa Fluor™ Plus 594] Summary

• Specificity: Detects human, mouse, and rat full length and mitochondria-processed HTRA2/Omi.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HTRA2/Omi Antibody [Alexa Fluor™ Plus 594]

• High temperature requirement protein A2
• HtrA serine peptidase 2
• HTRA2
• HtrA-like serine protease
• Omi
• OMIOmi stress-regulated endoprotease
• PARK13EC 3.4.21.108
• PRSS25
• PRSS25serine, 25
• Serine protease 25
• serine protease HTRA2, mitochondrial
• Serine proteinase OMI

Background

HtrA2/Omi is the mammalian homologue of bacterial high temperature requirement protein (HtrA). HtrA2/Omi localizes to the mitochondria and is processed to expose an amino-terminal Reaper-like motif similar to SMAC/Diablo. HtrA2/Omi is released from the mitochondria in response to apoptotic insult and can interact with the BIR2 or BIR3 domains of XIAP to relieve caspase-IAP inhibition. This effect can be measured by reversing XIAP-BIR2 (R&D Systems, Catalog # 786-XB) inhibition of Caspase-7 (R&D Systems, Catalog # 823-C7) cleavage of a fluorogenic peptide (DEVD-AFC, MP Bio, Catalog # AFC-138). IC₅₀ values for this effect are typically between 0.2 and 1.5 μM. HtrA2/Omi is trimeric and functions as a serine protease. The serine protease activity may play a more central role in apoptosis than its IAP antagonizing function. A PDZ domain regulates the serine protease activity by blocking access to the active site. The specificity of the protease is yet to be defined and no endogenous substrates are known to date.

1. Suzuki, Y. et al. (2001) Mol. Cell. 8:613.
2. van Loo, G. et al. (2002) Cell Death & Diff. 9:20.
3. Hedge, R. et al. (2001) J. Biol. Chem. 277:432.
4. Verhagen, A. et al. (2001) J. Biol. Chem. 277:445.
5. Martins, L. et al. (2002) J. Biol. Chem. 277:439.
6. Silke, J., and A. Verhagen (2002) Cell Death & Diff. 9:362.
7. Savopoulos, J. et al. (2000) Protein Expression & Purification 19:227.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.