HSPA2 Antibody (2D11) - Azide and BSA Free

Western Blot: HSPA2 Antibody (2D11) [H00003306-M03] - Analysis of HSPA2 expression in HepG2 (Cat # L019V1).

Immunoprecipitation: HSPA2 Antibody (2D11) [H00003306-M03] - Analysis of HSPA2 transfected lysate using anti-HSPA2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HSPA2 rabbit polyclonal antibody.

Sandwich ELISA: HSPA2 Antibody (2D11) [H00003306-M03] - Detection limit for recombinant GST tagged HSPA2 is approximately 3ng/ml as a capture antibody.

• Reactivity: Hu, Rt
• Applications: WB, ELISA, IP
• Clone: 2D11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

HSPA2 Antibody (2D11) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: HSPA2 (AAH01752, 1 a.a. ~ 639 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSARGPAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTIFDAKRLIGRKFGDATVQSDMKHWPFRVVSEGGKPKVQVEYKGETKTFFPEEISSMVLTKMKEIAEAYLGGKVHSAVITVPAYFNDSQRQATKDAGTITGLNVLRIINEPTAAAIAYGLDKKGCAGGEKNVLIFDLGGGTFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVSHLAEEFKRKHKKDIGPNKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGVDFYTSITRARFEELNADLFRGTLEPVEKALRDAKLDKGQIQEIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILIGDKSENVQDLLLLDVTPLSLGIETAGGVMTPLIKRNTTIPTKQTQTFTTYSDNQSSVLVQVYEGERAMTKDNNLLGKFDLTGIPPAPRGVPQIEVTFDIDANGILNVTAADKSTGKENKITITNDKGRLSKDDIDRMVQEAERYKSEDEANRDRVAAKNALESYTYNIKQTVEDEKLRGKISEQDKNKILDKCQEVINWLDRNQMAEKDEYEHKQKELERVCNPIISKLYQGGPGGGSGGGGSGASGGPTIEEVD
• Specificity: HSPA2 - heat shock 70kDa protein 2
• Isotype: IgG3 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HSPA2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for HSPA2 Antibody (2D11) - Azide and BSA Free

• Heat shock 70 kDa protein 2
• heat shock 70kD protein 2
• heat shock 70kDa protein 2
• heat shock-related 70 kDa protein 2
• HSP70-2
• HSP70-3
• HSPA2

Background

In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediatethe folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participatein all these processes through their ability to recognize nonnative conformations of other proteins. They bindextended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membranetranslocation, or following stress-induced damage

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HSPA2 Antibody (H00003306-M03)(1)

Publication using H00003306-M03

• Han DY, Kang SR, Park OS et al. Polychlorinated biphenyls have inhibitory effect on testicular steroidogenesis by downregulation of P45017{alpha} and P450scc. Toxicol Ind Health. 2010-03-31 [PMID: 20356859]

Bioinformatics

• Gene Symbol: HSPA2
• Entrez:
  • 3306(Human)
• OMIM:
  • 140560(Human)
• Uniprot:
  • AAH01752(Human)