HspA1B Antibody - BSA Free

Western Blot: HspA1B Antibody [NBP2-16896] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hsp70 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: HspA1B Antibody [NBP2-16896] - Mouse testis. Hsp70 stained by Hsp70 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: HspA1B Antibody [NBP2-16896] - Human breast cancer. Hsp70 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: HspA1B Antibody [NBP2-16896] - Rat brain. Hsp70 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry Whole-Mount : HspA1B Antibody [NBP2-16896] -Paraformaldehyde-fixed 1 day-post-fertilization zebrafish embryo.Green: Hsp70 stained by Hsp70 antibody diluted at 1:100.Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

Immunocytochemistry/ Immunofluorescence: HspA1B Antibody [NBP2-16896] - HspA1B antibody detects HspA1B protein at cytoplasm by immunofluorescent analysis.Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HspA1B stained by HspA1B antibody (NBP2-16896) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .

Immunohistochemistry Whole-Mount: HspA1B Antibody [NBP2-16896] -Paraformaldehyde-fixed 1 day-post-fertilization zebrafish embryo.Green: Hsp70 stained by Hsp70 antibody diluted at 1:100.Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

• Reactivity: Hu, Mu, Rt, Ze
• Applications: WB, ICC/IF, IHC, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

HspA1B Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human HspA1B. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HSPA1B
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Assay dependent
  • Immunohistochemistry Whole-Mount Assay dependent
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (89%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for HspA1B Antibody - BSA Free

• FLJ54328
• Heat shock 70 kDa protein 1/2
• heat shock 70 kDa protein 1A/1B
• heat shock 70kD protein 1B
• heat shock 70kDa protein 1B
• HSP70.1/HSP70.2
• HSP70-1/HSP70-2
• HSP70-1B
• HSP70-2
• HSPA1
• HSPA1A

Background

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for HspA1B Antibody (NBP2-16896)(3)

Publications using NBP2-16896

• Onay Ucar E, Sengelen A, Mertoglu Kamali E Hsp27, Hsp60, Hsp70, or Hsp90 depletion enhances the antitumor effects of resveratrol via oxidative and ER stress response in human glioblastoma cells Biochemical Pharmacology 2023-02-01 [PMID: 36603687] (WB, Human)
  
  Details:
  1:1000 WB dilution
• Kiyga E, Adıguzel Z, Onay Ucar E Temozolomide increases heat shock proteins in extracellular vesicles released from glioblastoma cells Molecular biology reports 2022-06-25 [PMID: 35752701] (WB, Human)
• Xia S, Li X, Xu S et al. Sublethal heat treatment promotes breast cancer metastasis and its molecular mechanism revealed by quantitative proteomic analysis Aging 2022-02-12 [PMID: 35150481] (IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: HSPA1B
• Uniprot:
  • P08107()