HSP70/HSPA1A Antibody (BB70)

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Western Blot: HSP70/HSPA1A Antibody (BB70) [NB110-96427] - analysis of Human HeLa cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Primary Antibody: Mouse ...read more
Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (BB70) [NB110-96427] - Tissue: hepatocytes. Species: Rat. Fixation: Paraffin Embedded. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:200. Courtesy of: ...read more
Western Blot: HSP70/HSPA1A Antibody (BB70) [NB110-96427] - analysis of Bovine MDBK cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Primary Antibody: Mouse ...read more
Immunohistochemistry: HSP70/HSPA1A Antibody (BB70) [NB110-96427] - Detection in paraffin embedded liver sections and nuclear sections of rat hepatocytes by staining with BB70. First pictures in series show two hours ...read more
Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (BB70) [NB110-96427] - analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: ...read more
Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (BB70) [NB110-96427] - Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:10000 for 12 hours at ...read more

Product Details

Summary
Reactivity ChSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clone
BB70
Clonality
Monoclonal
Host
Mouse
Concentration
1 mg/ml

Order Details

HSP70/HSPA1A Antibody (BB70) Summary

Immunogen
Proteins corresponding to the molecular mass of inducible Hsp and Hsc on SDS PAGE immunoblots.HSP70/HSP90 complex (NP_001006686.1)
Localization
Cytoplasm
Specificity
Detects approx 72 (HSP) and approx 73kDa (HSC).
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
HSPA1A
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
PBS (pH 7.2) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
1 ug/ml of HSP70/HSC70 Antibody was sufficient for detection of HSP70 and HSC70 in 20 ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Based on homology, It is predicted to detect Human, Mouse, Rat, Bovine, Sheep, Canine, Beluga, Fish, Guinea Pig, Porcine, Hamster, Rabbit, Xenopus, Drosophila and Yeast

Alternate Names for HSP70/HSPA1A Antibody (BB70)

  • dnaK-type molecular chaperone HSP70-1
  • FLJ54303
  • FLJ54370
  • FLJ54392
  • FLJ54408
  • FLJ75127
  • Heat shock 70 kDa protein 1/2
  • heat shock 70 kDa protein 1A/1B
  • heat shock 70kD protein 1A
  • heat shock 70kDa protein 1A
  • heat shock-induced protein
  • HSP70.1/HSP70.2
  • HSP70/HSPA1A
  • HSP70-1
  • HSP70-1/HSP70-2
  • HSP70-1A
  • HSP70I
  • HSP72
  • HSPA1
  • HSPA1A
  • HSPA1B

Background

Hsp70 genes encode abundant heat-inducible 70-kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (2). The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport..

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol HSPA1A
Entrez
Uniprot

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