HSP70/HSPA1A Antibody (998953) [Unconjugated]

Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 42° C heat shock for 30 minutes and allowed to recover for 3 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat HSP70/HSPA1A Monoclonal Antibody (Catalog # MAB16631) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HSP70/HSPA1A at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western blot shows lysates of human liver tissue, mouse liver tissue, and rat liver tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat HSP70/HSPA1A Monoclonal Antibody (Catalog # MAB16631) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HSP70/HSPA1A at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

HSP70/HSPA1A was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Mouse Anti-Human/Mouse/Rat HSP70/HSPA1A Monoclonal Antibody (Catalog # MAB16631) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

HSP70/HSPA1A was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Mouse Anti-Human/Mouse/Rat HSP70/HSPA1A Monoclonal Antibody (Catalog # MAB16631) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 42oC heat shocked (HS) for 30 minutes and allowed to recover for 3 hours, loaded at 0.2 mg/mL. A specific band was detected for HSP70/HSPA1A at approximately 70 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human HSP70/HSPA1A Monoclonal Antibody (Catalog # MAB16631) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, IHC, ICC/IF
• Clone: 998953
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

HSP70/HSPA1A Antibody (998953) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human HSP70/HSPA1A
  Met1-Asp641
  Accession # P0DMV8 and P0DMN9
• Specificity: Detects human HSP70/HSPA1A in direct ELISAs. Detects, human, mouse, and rat HSP70/HSPA1A in Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 5-25 ug/mL
  • Simple Western 20 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HSP70/HSPA1A Antibody (998953) [Unconjugated]

• dnaK-type molecular chaperone HSP70-1
• FLJ54303
• FLJ54370
• FLJ54392
• FLJ54408
• FLJ75127
• Heat shock 70 kDa protein 1/2
• heat shock 70 kDa protein 1A/1B
• heat shock 70kD protein 1A
• heat shock 70kDa protein 1A
• heat shock-induced protein
• HSP70
• HSP70.1/HSP70.2
• HSP70-1
• HSP70-1/HSP70-2
• HSP70-1A
• HSP70I
• HSP72
• HSPA1
• HSPA1A
• HSPA1B

Background

Heat shock proteins (HSPs) are a family of highly conserved stress response proteins. Heat shock proteins function primarily as molecular chaperones by facilitating the folding of other cellular proteins, preventing protein aggregation or targeting improperly folded proteins to specific degradative pathways. HSPs are typically expressed at low levels under normal physiological conditions but are dramatically up‑regulated in response to cellular stress. HSP70 is a 72 kDa member of the heat shock protein 70 family of proteins.  HSP70, also known as HSPA1A, HSP70-1, and HSP72 is a 641 amino acid (aa) heat shock protein. Over aa 1-641, human HSP70 shares 95% and 97% aa identity to mouse and rat HSP70.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P0DMV8 and P0DMN9()