HSP60 Antibody

Images

 
Western Blot: HSP60 Antibody [NBP2-12734] - analysis of Parasite Lysates showing detection of HSP60 protein using Rabbit Anti-HSP60 Polyclonal Antibody. Primary Antibody: Rabbit Anti-HSP60 Polyclonal Antibody at 1:1000.

Product Details

Summary
Reactivity PfaSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Concentration
1.83 mg/ml

Order Details

HSP60 Antibody Summary

Immunogen
Recombinant full length of HSP60 protein antibody detects an 60kD protein, corresponding to P. Falciparum Hsp60 on SDS PAGE immunoblot; in samples from P. Falciparum origin.
Localization
Mitochondrion, Mitochondrion Matrix
Marker
Mitochondria Marker
Specificity
Detects approx 60kDa. Cross-reacts with E.coli HSP60, GroEl.
Clonality
Polyclonal
Host
Rabbit
Gene
HSPD1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
PBS (pH 7.4) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1.83 mg/ml
Purity
Protein A purified

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
0.9 ug/ml of HSP60 (P. falciparum) Antibody was sufficient for detection of PfHSP60 in 20 ug of P. falciparum lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary Antibody.

Reactivity Notes

Based on homology, It's predicted to detect Bacteria E. coli (Escherichia coli).

Alternate Names for HSP60 Antibody

  • 60 kDa chaperonin
  • Chaperonin 60
  • cpn60
  • GROEL
  • heat shock 60kD protein 1 (chaperonin)
  • heat shock 60kDa protein 1 (chaperonin)
  • Heat shock protein 60
  • heat shock protein 65
  • HLD4
  • HSP60
  • HSP-60
  • HSP60SPG13
  • HSP65
  • HSPD1
  • HuCHA60
  • Mitochondrial matrix protein P1,60 kDa heat shock protein, mitochondrial
  • P60 lymphocyte protein
  • short heat shock protein 60 Hsp60s1
  • spastic paraplegia 13 (autosomal dominant)
  • SPG13

Background

Certificate of Analysis, 0.9 ug/mL was sufficient for detection of PfHsp60 in 20ug of P. falciparum lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG: HRP as the secondary antibody.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HSP60 Antibody (NBP2-12734) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for HSP60 Antibody (NBP2-12734)

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Blogs on HSP60.

TLR4 - A Guardian of Innate Immunity
Toll-like receptor 4 (TLR4) belongs to the family of Toll-like receptors (TLR), and plays a main role in pathogen recognition and innate immunity system activation. The TLR family members are highly conserved proteins that all contain a high degree of...  Read full blog post.

Customer Experience using HSP60 Antibody
I began using the HSP60 antibody (NB110-57063) in June of 2010 and it worked well. I do not like to buy antibodies that have not been tested in the species for which I will use them, so I picked this antibody because it had already been tested in rat...  Read full blog post.

Heat Shock Proteins: An Overview
Heat Shock Proteins (HSPs) are a ubiquitous group of molecular chaperone proteins that have evolved unique mechanisms, within their host cells, to facilitate survival in hostile environments such as heat, oxidative (hypoxia), pH and cold. Under permis...  Read full blog post.

The Heat is On: Heat Shock Proteins and the Link to Cancer
Novus Biologicals offers an extensive antibody catalog targeting heat shock proteins (HSPs). A large protein group covering a number of families, the HSPs are functionally related by their dramatic upregulation in response to stress. Stress triggers m...  Read full blog post.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol HSPD1
Entrez

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