HR6A/UBE2A Antibody

Western Blot: HR6A/UBE2A Antibody [NB100-555] - Detection of Human Rad6 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rad6 antibody NB100-555 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Western Blot: HR6A/UBE2A Antibody [NB100-555] - Whole cell lysate (50 ug) from 293T cells.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

HR6A/UBE2A Antibody Summary

• Immunogen: A synthetic peptide which maps to a region between residues 100 and 150 of human ubiquitin-conjugating enzyme E2A using the numbering given in Swiss-Prot entry P49459 (GeneID 7319).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UBE2A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:5000-1:25000
• Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: 1:5,000 - 1:25,000 Immunoprecipitation: not tested Histo/Cytochemistry: not tested *In some cases, the antibody may be diluted further than indicated.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HR6A/UBE2A Antibody

• EC 6.3.2.19
• HHR6A
• HR6A
• RAD6A
• RAD6ARAD6 homolog A
• UBC2
• UBE2A
• Ubiquitin carrier protein A
• ubiquitin-conjugating enzyme E2 A
• ubiquitin-conjugating enzyme E2A (RAD6 homolog)
• Ubiquitin-protein ligase A

Background

The rad6 protein catalyzes the monoubiquitination of H2B. Rad6-directed H2B ubiquitination defines regions of active chromatin. The RAD6 pathway is also central to post-replicative DNA repair in eukaryotic cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UBE2A
• Entrez:
  • 7319(Human)
• Uniprot:
  • P49459(Human)