Rat HO-1/HMOX1/HSP32 ELISA Kit (Colorimetric)

ELISA: Rat HO-1/HMOX1/HSP32 ELISA Kit (Colorimetric) [NBP2-62148] - A typical standard curve for HO-1/HMOX1/HSP32 ELISA kit. These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Product Discontinued

Rat HO-1/HMOX1/HSP32 ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 0.195 - 12.5 ng/ml
• Sensitivity: 0.036 ng/ml
• Assay Type: Sandwich ELISA
• Inter-Assay: The Inter-Assay Coefficient of Variation of the HO-1 (rat), ELISA kit has been determined to be < 10%
• Intra-Assay: The Intra-Assay Coefficient of Variation of the HO-1 (rat), ELISA kit has been determined to be < 4%.
• Spike Recovery: The % recoveries for cell lysates, tissue extracts, serum, and plasma were > 90%.
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: HMOX1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat HO-1/HMOX1/HSP32 ELISA Kit (Colorimetric)

• bK286B10
• EC 1.14.99.3
• heat shock protein, 32-kD
• heme oxygenase (decycling) 1
• HMOX1
• HO
• HO1
• HO-1
• HO-1heme oxygenase 1
• HSP32

Background

Stressed mammalian cells up-regulate heme oxygenase 1 (HO-1), which catabolizes heme to biliverdin, carbon monoxide, and free iron. Results provide genetic evidence that up-regulation of HO-1 serves as an adaptive mechanism to protect cells from oxidative damage during stress (1). When cells are injured they release their contents, resulting in a local accumulation of free heme proteins and heme. HO-1 plays a crucial role in the inflammatory process during wound healing (2). TNFalpha accelerates inflammatory responses by down-regulating HO-1 expression in human monocytes. TNF antagonists may block this TNF-dependent suppression of HO-1 expression, resulting in an amelioration of inflammation (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for HO-1/HMOX1/HSP32 ELISA Kit (NBP2-62148)(1)

Publication using NBP2-62148

• Tigges J, Worek F, Thiermann H, Wille T Organophosphorus pesticides exhibit compound specific effects in rat precision-cut lung slices (PCLS): mechanisms involved in airway response, cytotoxicity, inflammatory activation and antioxidative defense Archives of toxicology 2021-11-15 [PMID: 34778934] (ELISA)

FAQs for HO-1/HMOX1/HSP32 ELISA Kit (NBP2-62148). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: HMOX1