HO-1/HMOX1/HSP32 Antibody (GTS-1)

Product Discontinued

HO-1/HMOX1/HSP32 Antibody (GTS-1) Summary

• Immunogen: Rat heme oxygenase 1.
• Localization: Microsome. Endoplasmic reticulum
• Marker: Oxidative Stress Marker
• Specificity: It does not react with heme oxygenase 2.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HMOX1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • ELISA 1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 5-10 ug/ml
  • Immunohistochemistry-Paraffin 5-10 ug/ml
  • Western Blot 5-10 ug/ml
• Application Notes: This antibody will inhibit the activity of human or rat heme oxygenase 1.
• Control:

  HO-1/HMOX1/HSP32 Overexpression Lysate

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.4) and 1.0% BSA
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for HO-1/HMOX1/HSP32 Antibody (GTS-1)

• bK286B10
• EC 1.14.99.3
• heat shock protein, 32-kD
• heme oxygenase (decycling) 1
• HMOX1
• HO
• HO1
• HO-1
• HO-1heme oxygenase 1
• HSP32

Background

Heme oxygenase (HO) is a microsomal enzyme that catalyzes the oxidation of heme to the antioxidant molecules, biliverdin and carbon monoxide. HO consists of two homologous isozymes, an inducible HO1 and a constitutively expressed HO2. HO1 is induced by a wide variety of stimuli including conditions of oxidative stress, inflammatory agents, transforming growth factor beta and heat shock. The increase in expression of HO1 is thought to be a cellular defense mechanism against oxidative stress since elevated HO could eventually generate more bilirubin, an anti-oxidant. HO1 expression is greatly increased in neurofibrillary tangles in Alzheimer's disease and other neurodegenerative diseases such as progressive supranuclear palsy and subacute sclerosing panencephalitis. Inhibition of HO1 is associated with tissue pathology in atherosclerosis and other inflammatory conditions associated with intravascular thrombosis such as septic shock, hypoxia, and graft rejection.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HO-1/HMOX1/HSP32 Antibody (NB110-10991)(3)

Publications using NB110-10991

• Krautbauer S, Eisinger K, Hader Y et al. Manganese superoxide dismutase knock-down in 3T3-L1 preadipocytes impairs subsequent adipogenesis. Mol. Cell. Biochem. 2014-04-17 [PMID: 24740755] (WB, Mouse)
• Machado C, Malheiros DM, Adamy A et al. Protective response in renal transplantation: no clinical or molecular differences between open and laparoscopic donor nephrectomy. Clinics (Sao Paulo). 2013-04-01 [PMID: 23778338] (IHC-P, Human)
• Zhang L, Gavin T, Barber DS, LoPachin RM. Role of the Nrf2-ARE pathway in acrylamide neurotoxicity. Toxicol Lett;205(1):1-7. 2011-08-10 [PMID: 21540084] (WB, Rat)

Bioinformatics

• Gene Symbol: HMOX1
• Entrez:
  • 3162(Human)
  • 24451(Mouse)
  • 15368(Rat)