HO-1/HMOX1/HSP32 Antibody (6B8-2F2) [Alexa Fluor® 647]

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: 6B8-2F2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

HO-1/HMOX1/HSP32 Antibody (6B8-2F2) [Alexa Fluor® 647] Summary

• Immunogen: His-tagged Rat HO-1
• Localization: Microsome , Endoplasmic Reticulum
• Specificity: Detects 32kDa. Does not cross-react with HO-2.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HMOX1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for HO-1/HMOX1/HSP32 Antibody (6B8-2F2) [Alexa Fluor® 647]

• bK286B10
• EC 1.14.99.3
• heat shock protein, 32-kD
• heme oxygenase (decycling) 1
• HMOX1
• HO
• HO1
• HO-1
• HO-1heme oxygenase 1
• HSP32

Background

Stressed mammalian cells up-regulate heme oxygenase 1 (HO-1), which catabolizes heme to biliverdin, carbon monoxide, and free iron. Results provide genetic evidence that up-regulation of HO-1 serves as an adaptive mechanism to protect cells from oxidative damage during stress (1). When cells are injured they release their contents, resulting in a local accumulation of free heme proteins and heme. HO-1 plays a crucial role in the inflammatory process during wound healing (2). TNFalpha accelerates inflammatory responses by down-regulating HO-1 expression in human monocytes. TNF antagonists may block this TNF-dependent suppression of HO-1 expression, resulting in an amelioration of inflammation (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HMOX1