hnRNP H Antibody - Azide and BSA Free

Western Blot: hnRNP H Antibody [NBP2-93590] - Western blot analysis of extracts of various cell lines, using hnRNP H antibody (NBP2-93590) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunocytochemistry/Immunofluorescence: hnRNP H Antibody [NBP2-93590] - Immunofluorescence analysis of 293T cells using hnRNP H antibody (NBP2-93590) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: hnRNP H Antibody [NBP2-93590] - Immunohistochemistry of paraffin-embedded rat ovary using hnRNP H Rabbit pAb (NBP2-93590) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Western Blot: hnRNP H Antibody [NBP2-93590] - Western blot analysis of extracts of various cell lines, using hnRNP H Rabbit pAb (NBP2-93590) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunocytochemistry/Immunofluorescence: hnRNP H Antibody [NBP2-93590] - Analysis of 293T cells using hnRNP H . Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: hnRNP H Antibody [NBP2-93590] - Immunohistochemistry of paraffin-embedded human colon carcinoma using hnRNP H Rabbit pAb (NBP2-93590) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: hnRNP H Antibody [NBP2-93590] - Immunohistochemistry of paraffin-embedded mouse kidney using hnRNP H Rabbit pAb (NBP2-93590) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

hnRNP H Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit hnRNP H Antibody - Azide and BSA Free (NBP2-93590) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 191-449 of human hnRNP H (NP_005511.1). VRTHYDPPRKLMAMQRPGPYDRPGAGRGYNSIGRGAGFERMRRGAYGGGYGGYDDYNGYNDGYGFGSDRFGRDLNYCFSGMSDHRYGDGGSTFQSTTGHCVHMRGLPYRATENDIYNFFSPLNPVRVHIEIGPDGRVTGEADVEFATHEDAVAAMSKDKANMQHRYVELFLNSTAGASGGAYEHRYVELFLNSTAGASGGAYGSQMMGGMGLSNQSSYGGPASQQLSGGYGGGYGGQSSMSGYDQVLQENSSDFQSNIA
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HNRNPH1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000 - 1:5000
• Theoretical MW: 49 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for hnRNP H Antibody - Azide and BSA Free

• DKFZp686A15170
• heterogeneous nuclear ribonucleoprotein H
• heterogeneous nuclear ribonucleoprotein H1 (H)
• hnRNP H
• HNRPH
• HNRPH1hnRNPH

Background

The heterogeneous nuclear Ribonucleoprotein (hnRNP) H protein family is involved in pre-mRNA processing. It is essential for enhancer activity. The sequence GGGA is recognized by all hnRNP H family proteins. Mutant exon 6D sequences function as a splicing enhancer when inserted into an enhancer-dependent splicing construct. hnRNP H family proteins bind to the enhancer and their binding is dependent on the sequence GGGA, located just downstream of the point of mutation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: HNRNPH1