hnRNP F Antibody (OTI5F5) - Azide and BSA Free

Western Blot: hnRNP F Antibody (OTI5F5) - Azide and BSA Free [NBP2-70907] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY hnRNP F (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-hnRNP F.

Immunocytochemistry/Immunofluorescence: hnRNP F Antibody (OTI5F5) - Azide and BSA Free [NBP2-70907] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY hnRNP F.

Immunohistochemistry: hnRNP F Antibody (OTI5F5) - Azide and BSA Free [NBP2-70907] - Staining of paraffin-embedded Human liver tissue using anti-hnRNP F mouse monoclonal antibody.

Flow Cytometry: hnRNP F Antibody (OTI5F5) - Azide and BSA Free [NBP2-70907] - Analysis of Hela cells, using anti-hnRNP F antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Immunocytochemistry/Immunofluorescence: hnRNP F Antibody (OTI5F5) - Azide and BSA Free [NBP2-70907] - Staining of HepG2 cells using anti-hnRNP F mouse monoclonal antibody.

Immunohistochemistry: hnRNP F Antibody (OTI5F5) - Azide and BSA Free [NBP2-70907] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-hnRNP F mouse monoclonal antibody.

Immunohistochemistry: hnRNP F Antibody (OTI5F5) - Azide and BSA Free [NBP2-70907] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-hnRNP F mouse monoclonal antibody.

Immunohistochemistry: hnRNP F Antibody (OTI5F5) - Azide and BSA Free [NBP2-70907] - Staining of paraffin-embedded Human Kidney tissue using anti-hnRNP F mouse monoclonal antibody.

Immunohistochemistry: hnRNP F Antibody (OTI5F5) - Azide and BSA Free [NBP2-70907] - Staining of paraffin-embedded Human prostate tissue using anti-hnRNP F mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI5F5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

hnRNP F Antibody (OTI5F5) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human HNRNPF (NP_004957) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: HNRNPF
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 45.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for hnRNP F Antibody (OTI5F5) - Azide and BSA Free

• heterogeneous nuclear ribonucleoprotein F
• hnRNP F
• HNRPFHnRNP F protein
• mcs94-1
• MGC110997
• Nucleolin-like protein mcs94-1
• OK/SW-cl.23

Background

Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to pre-mRNA processing and transport, and also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II. hnRNP complexes are the major constituents of the spliceosome and in particular, the hnRNP A1 protein is one of the major premRNA/ mRNA binding proteins and also one of the most abundant proteins in the nucleus. hnRNP A1 and A2/B1 regulate the processing of pre-mRNA by directly antagonizing the association of various splicing factors and by influencing the splice site selection on pre-mRNA. The majority of hnRNP proteins components are localized to the nucleus; however some shuttle between the nucleus and the cytoplasm. Most hnRNP proteins, including hnRNP C1 and C2, contain one or more RNA binding domains and are implicated in the processing of pre-mRNA. hnRNPs F and H are largely related factors that preferentially associate with poly(rG) regions on RNA. Isoforms of these proteins are often generated by alternative processing of the premRNA and by posttranslational modifications such as phosphorylation on serines and threonines and methylation of arginines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HNRNPF