hnRNP F Antibody (OTI5F5)

Western Blot: hnRNP F Antibody (5F5) [NBP2-02221] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY hnRNP F (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-hnRNP F.

Immunocytochemistry/Immunofluorescence: hnRNP F Antibody (5F5) [NBP2-02221] Staining of COS7 cells transiently transfected by pCMV6-ENTRY hnRNP F.

Immunohistochemistry-Paraffin: hnRNP F Antibody (5F5) [NBP2-02221] - Staining of paraffin-embedded Human prostate tissue using anti-hnRNP F mouse monoclonal antibody.

Flow Cytometry: hnRNP F Antibody (5F5) [NBP2-02221] - Analysis of Hela cells, using anti-hnRNP F antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Immunocytochemistry/Immunofluorescence: hnRNP F Antibody (5F5) [NBP2-02221] - Immunofluorescent staining of HepG2 cells using anti-hnRNP F mouse monoclonal antibody.

Immunohistochemistry-Paraffin: hnRNP F Antibody (5F5) [NBP2-02221] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-hnRNP F mouse monoclonal antibody.

Immunohistochemistry-Paraffin: hnRNP F Antibody (5F5) [NBP2-02221] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-hnRNP F mouse monoclonal antibody.

Immunohistochemistry-Paraffin: hnRNP F Antibody (5F5) [NBP2-02221] - Staining of paraffin-embedded Human Kidney tissue using anti-hnRNP F mouse monoclonal antibody.

Immunohistochemistry-Paraffin: hnRNP F Antibody (5F5) [NBP2-02221] - Staining of paraffin-embedded Human liver tissue using anti-hnRNP F mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI5F5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

hnRNP F Antibody (OTI5F5) Summary

• Immunogen: Full length human recombinant protein of human HNRNPF (NP_004957) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: HNRNPF
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:2000
• Theoretical MW: 45.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for hnRNP F Antibody (OTI5F5)

• heterogeneous nuclear ribonucleoprotein F
• hnRNP F
• HNRPFHnRNP F protein
• mcs94-1
• MGC110997
• Nucleolin-like protein mcs94-1
• OK/SW-cl.23

Background

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HNRNPF
• Entrez:
  • 3185(Human)